JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2009, Vol. 33 ›› Issue (04): 8-12.doi: 10.3969/j.jssn.1000-2006.2009.04.002

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Differentially expressed gene analysis of populus induced by Tetra lobulifera(Keifer)

ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu   

  1. Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China
  • Online:2009-08-18 Published:2009-08-18

Abstract: A suppression subtractive hybridization(SSH) cDNA library was constructed with Populus deltoides induced by Tetra lobulifera(Keifer). Realtime PCR was performed to confirm the candidate genes. In this study, six differentially expressed genes were found to be upregulated. Subsequently, the fulllong cDNA sequence about 2 053 bp was cloned by the RACE(Rapid Amplification of cDNA Ends) and named PtWRKY, which encod 588 amino acids.

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