Application of ITS sequence and SSR markers to study the relationship between Juglans regia and Juglans sigillata

WANG Hua1, YAN Yabo2, ZHANG Junpei1, PEI Dong1*

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2009, Vol. 33 ›› Issue (06) : 35.

南京林业大学学报（自然科学版）
Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2009, Vol. 33 ›› Issue (06) : 35. DOI: 10.3969/j.jssn.1000-2006.2009.06.008

Application of ITS sequence and SSR markers to study the relationship between Juglans regia and Juglans sigillata

  • WANG Hua1, YAN Yabo2, ZHANG Junpei1, PEI Dong1*
Author information +
History +

Abstract

Juglans regia L. and J.sigillata Dode. are two mainlycultivated walnut species in China, and the later is a unique species distributed in the southwest China. Furthermore, the argument about relationship of the two species remains undetermined. Five populations of J.regia and J.sigillata were chosen to investigate their relationship with ITS and SSR markers. Result of ITS sequence only detected 1 nucleotide difference between the two species. Based on 65 alleles generated with SSR, genetic distance between populations was calculated. The closest genetic distance was 0.119 found between SJC and QZ population, and the furthest distance was 0.552 found between MN and HS population. The genetic distance between intraspecies was similar to that between interspecies, and the difference between two species was quite small and was not significant. Those results suggested that the two species might be different ecotypes, which provided a scientific reference for revealing the evolutional and genetic relationship and genetic characteristics of walnut plants.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
WANG Hua1, YAN Yabo2, ZHANG Junpei1, PEI Dong1*. Application of ITS sequence and SSR markers to study the relationship between Juglans regia and Juglans sigillata[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2009, 33(06): 35 https://doi.org/10.3969/j.jssn.1000-2006.2009.06.008

References

[1]郗荣庭,张毅萍. 中国果树志·核桃卷[M]. 北京:中国林业出版社,1996.
[2]王红霞,张志华,玄立春. 我国核桃种质资源及育种研究进展[J]. 河北林果研究,2007,22(4):387-392.
[3]曲泽洲,孙云蔚. 果树种类论[M]. 北京:农业出版社,1990.
[4]杨自湘,奚声珂. 胡桃属十种植物的过氧化物同工酶分析[J]. 植物分类学报,1989,27 (1):53-57.
[5]匡可任,路安民. 中国植物志:第21 卷[M]. 北京:科学出版社,1979.
[6]吴燕民,裴东,奚声珂,等. 运用RAPD对核桃属种间亲缘关系的研究[J]. 园艺学报,2000,27(1):17-22.
[7]郗荣庭,张毅萍. 中国核桃[M].北京:中国林业出版社,1992.
[8]邹喻萍,葛颂,王晓东. 系统与进化植物学中的分子标记[M]. 北京:科学出版社,2001.
[9]田欣,李德铢. DNA序列在植物系统学研究中的应用[J]. 云南植物研究,2002,24(2):170-184.
[10]Stanford M A, Rachel H, Clifford R P. Phylogeny and biogeography of Juglans (Juglandaceae) based on mATK and ITS sequence data[J]. American Journal of Botany, 2000, 87(6): 872-882.
[11]Mallikarjuna K, Aradhya D P, Gao F Y, et al. Molecular phylogeny of Juglans (Juglandaceae): a biogeographic perspective[J]. Tree Genetics & Genomes, 2007, 3: 363-378.
[12]Lefort F, Douglas G C. An efficient micro–method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus[J]. Annals of Forest Science, 1999, 56: 259-263.
[13]Woeste K, Burns R, Rhodes O E, et al. Thirty polymorphic nuclear microsatellite loci from black walnut[J]. Journal of Heredity, 2002, 93: 58-60.
[14]Foroni I, Rao R, Woeste K, et al. Characterisation of Juglans regia L. with SSR markers and evaluation of genetic relationships among cultivars and the ’Sorrento’ landrace[J]. J Hort Sci & Biotechnol, 2005, 80(1): 49-53.
[15]Victory E R, Glaubitz J C, Rhodes Jr O E, et al. Genetic homogeneity in Juglans nigra (Juglandaceae) at nuclear microsatellites[J]. American Journal of Botany, 2006, 93(1): 118-126.
[16]Wang Y H, Luo J X, Xue X M. Diversity of microsatellite markers in the populations of Picea asperata originating from the mountains of China[J]. Plant Science, 2005, 168: 707-714.
[17]Panaud O, Chen X, McCouch S D. Development of microsatellite markers and characterization of sample sequence lengthen polymorphism (SSLP) in rice (Oryzastiva L.)[J]. Mol Gen Genet, 1996, 252: 597-607.
[18]Yeh F C, Yang R C, Boyle T. POPGENE version 1.32 ed. Software Microsoft Window–Based Freeware for Population Genetic Analysis[M]. Edmonton, Alta: University of Alberta Press, 1997.
[19]Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals[J]. Genetics, 1978, 89: 583-590.
[20]WANG H, PEI D, GU R S,et al. Genetic diversity and structure of walnut populations in central and southwestern China revealed by microsatellite markers[J]. Journal of America Society Horticulture Science, 2008, 133(2): 197-203.
[21]路安民,张志耘. 胡桃目的分化、进化和系统关系[J]. 植物分类学报,1990,28(2):96-102.
[22]Peng Y H, Lu Z, Chen K. Population genetic survey of Populus cathayana originating from southeastern Qinghai–Tibetan plateau of China based on SSR markers[J]. Silvae Genetica, 2005, 54(3): 116-122.
[23]范志远,方文亮,杨振邦,等. 南北核桃种间杂交F1代主要性状遗传分析[J]. 云南林业科技,2001(4):31-37.

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉