Cryopreservation of the pine wood nematode（Bursaphelenchus xylophilus）

doi:10.3969/j.jssn.1000-2006.2011.06.013

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2011, Vol. 35 ›› Issue (06): 63-66.doi: 10.3969/j.jssn.1000-2006.2011.06.013

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Cryopreservation of the pine wood nematode（Bursaphelenchus xylophilus）

ZHU Lihua, JI Jinyi, ZHANG Wenli, YE Jianren*

Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China

Online:2011-11-28 Published:2011-11-28

Abstract

Abstract: The cryopreservation of Bursaphelenchus xylophilus was investigated. The effects of different cryoprotectants (1 %-5 % DMSO, 5 %-25 % glycerol) on cryopreservation of nematodes and the fertility and infectivity of cryonematodes were evaluated. The results indicated that the survival rate of B. xylophilus was up to (39.4±6.6) % or (375±21.8) % after storage for 1 day at －80 ℃ by using 15 % glycerin or 4 % DMSO respectively as cryoprotectant. And it didnt decline significantly after storage for 10 days or a month. When cryopreserved nematodes were plated onto Botrytis cinerea, they reproduced rapidly. Cryopreserved nematodes were as pathogenic as untreated nematodes to Japanese black pines.

CLC Number:

S763

ZHU Lihua, JI Jinyi, ZHANG Wenli, YE Jianren*. Cryopreservation of the pine wood nematode（Bursaphelenchus xylophilus）[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2011, 35(06): 63-66.

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Cryopreservation of the pine wood nematode（Bursaphelenchus xylophilus）

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