Study on origin and development of somatic embryos of Liriodendron hybrids

doi:10.3969/j.jssn.1000-2006.2012.01.004

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2012, Vol. 36 ›› Issue (01): 16-20.doi: 10.3969/j.jssn.1000-2006.2012.01.004

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Study on origin and development of somatic embryos of Liriodendron hybrids

CHEN Jinhui，ZHANG Yanjuan，LI Tingting，WANG Pengkai，WANG Guangping，SHI Jisen*

Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China

Online:2012-01-30 Published:2012-01-30

Abstract

Abstract: This research was mainly to discover the origin and development of somatic embryos of Liriodendron hybrids with cellular observation. The results showed that embryogenic callus can be easily induced when used immature zygote embryo as explant. The embryogenic callus structure was tight and compacted and embryogenic cells were small, isodiametric and densely cytoplasmic, with a high nucleoplasmic ratio and small starch grains. Some singlecells from the surface of embryogenic callus can grow into singlecell proembryo with thick cell wall, which show they are independent at the beginning of their lives. The significant character of the somatic embryo was its polarity. Plantlet regenerations were developed from global embryo, heartshaped embryo, torpedoshaped embryo and cotyledon embryo, in which the Vshaped vessel vascular bundles, shoot tip meristem and root tip meristem were observed.

CLC Number:

S722
Q813

CHEN Jinhui，ZHANG Yanjuan，LI Tingting，WANG Pengkai，WANG Guangping，SHI Jisen*. Study on origin and development of somatic embryos of Liriodendron hybrids[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2012, 36(01): 16-20.

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Study on origin and development of somatic embryos of Liriodendron hybrids

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