Abstract: Effects of zinc application on seedling growth and zinc distribution in different organs of Carya illinoensis(Wangenh.)K. Koch were investigated using the one year-old seedling of C. illinoensis as the research object. Results showed that the seedling growth was promoted by spraying ZnSO₄ with a low concentration. In contrary, the seedling growth was inhibited by ZnSO₄ with a high concentration. The similar trend was also observed under nut-presoaking treatment. Leaf chlorophy Ⅱ content presented a trend “declining-increasing-declining rapidly” with ZnSO₄ rate increasingly. The ratio of zinc in pecan seedlings was significantly increased after leaf zinc spraying. It was found that the highest amount of zinc was detected in pecan leaves, whereas roots gathered the lowest. However, zinc content was the highest in shoot, the lowest in root when used nut-presoaking method. Summarily, 0.4% of foliar applied ZnSO₄ is recommended in Zn fertilization of pecan.