Abstract
Several factors influencing αglucosidase production with the recombinant Escherichia coli haroring the JM109/pBV220GLZ were investigated. The results showed that the best inducement conditions were as follows: induction temperature 40 ℃, induction time 14 h, and at OD600 of 2.0. To determine the optimum conditions of immobilization, the concentration of sodium alginate， CaCl2， and glutaraldehyde, and solidifying time were studied. The results showed that the optimum operations were: 20 g／L sodium alginate， 20 g／L CaCl2， 1.0 ％ glutaraldehyde， solidifying time 2 h. Under the optimum condition， the recovery rate reached 81 ％. After 15 times repeated operations， the enzyme activity remained 75 ％， showing good operational stability. Thus， the superior stability of the recombinant enzyme laid the base for largescale application.
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PEI Jianjun， L Xiangxiang， XIE Jingcong， ZHAO Linguo*.
Inducement condition and immobilization of thermostable αglucosidase produced by Escherichia coli[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2012, 36(06): 90-94 https://doi.org/10.3969/j.jssn.1000-2006.2012.06.018
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