The application of sitespecific DNA endonucleases in plant gene targeting

CHENG Qiang, XU Meng, HUANG Minren*

Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2012, Vol. 36 ›› Issue (06) : 130-134.

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Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2012, Vol. 36 ›› Issue (06) : 130-134. DOI: 10.3969/j.jssn.1000-2006.2012.06.026

The application of sitespecific DNA endonucleases in plant gene targeting

  • CHENG Qiang, XU Meng, HUANG Minren*
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Abstract

Gene targeting is a powerful tool for sitespecific insertion, deletion and replacement of DNA in genome. Gene targeting technology in plants is far from routine due to the low frequency of homologous recombination that limits the study of gene function and molecular breeding. Recently, breakthrough has been made in the engineered DNA binding domains combined with zinc finger protein and transcription activatorlike effector. Engineered DNA binding domain fusing endonucleases can specifically breaks the DNA doublestrand, then generate sitedirected mutagenesis and facilitate homologous recombination. In this review, we focus on the application of zinc finger nuclease and TAL effector nuclease in sitedirected mutagenesis and gene targeting of plant genome and analyze their existing problems.

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CHENG Qiang, XU Meng, HUANG Minren*. The application of sitespecific DNA endonucleases in plant gene targeting[J]. Journal of Nanjing Forestry University (Natural Sciences Edition). 2012, 36(06): 130-134 https://doi.org/10.3969/j.jssn.1000-2006.2012.06.026

References

[1]Sherry A Kempin, Sarah J Liljegren, Laura M Block, et al. Targeted disruption in Arabidopsis[J]. Nature,1997,389: 802-803.
[2]Jerzy Paszkowski, Markus Baur, Augustyn Bogucki, et al. Gene targeting in plants[J]. The EMBO Journal, 1988, 7(13): 4021-4026.
[3]Alonso M, Anna N Stepanova, Thomas J Leisse, et al. Genomewide insertional mutagenesis of Arabidopsis thaliana[J]. Science, 2003, 301: 653-657.
[4]Matthew H Porteus. Plant biotechnology: zinc fingers on target[J]. Nature, 2009, 459: 337-338.
[5]Tzvi Tzfira1, Dan Weinthal, Ira Marton, et al. Genome modifications in plant cells by custommade restriction enzymes[J]. Plant Biotechnology Journal, 2012, 10(4): 373-389.
[5]Fengli Fu, Jeffry D Sander, Morgan Maeder, et al. Zinc finger database (ZiFDB): a repository for information on C2H2 zinc fingers and engineered zincfinger arrays[J]. Nucleic Acids Research, 2009,37(1): 279-283.
[7]David A Wright, Stacey ThibodeauBeganny, Jeffry D Sander, et al. Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly[J]. Nature Protocols, 2006, 1(3): 1637-1652.
[8]Ankit Gupta, Ryan G Christensen, Amy L Rayla, et al. An optimized twofinger archive for ZFNmediated gene targeting[J]. Nature Methods, 2012, 9: 588-590.
[9]Morgan L Maeder, Stacey ThibodeauBeganny, Jeffry D Sander, et al. Oligomerized pool engineering (OPEN): an ‘opensource’ protocol for making customized zincfinger arrays[J]. Nature Protocols, 2009, 4(10): 1471-1501.
[10]Fyodor D Urnov, Edward J Rebar, Michael C Holmes, et al. Genome editing with engineered zinc finger nucleases[J]. Nature Reviews Genetics, 2010, 11(9): 636-646.
[11]Alan Lloyd, Christopher L Plaisier, Dana Carroll, et al. Targeted mutagenesis using zincfinger nucleases in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(6): 2232-2237.
[12]David A Wright, Jeffrey A Townsend, Ronnie Joe Winfrey Jr, et al. Highfrequency homologous recombination in plants mediated by zincfinger nucleases[J]. The Plant journal, 2005, 44(4): 693-705.
[13]Vipula K Shukla, Yannick Doyon, Jeffrey C Miller, et al. Precise genome modification in the crop species Zea mays using zincfinger nucleases[J]. Nature, 2009, 459: 437-441.
[14]Jeffrey A Townsend, David A Wright, Ronnie J Winfrey, et al. Highfrequency modification of plant genes using engineered zincfinger nucleases[J]. Nature, 2009,459: 442-445.
[15]Vikram Pattanayak, Cherie L Ramirez, J Keith Joung, et al. Revealing offtarget cleavage specificities of zincfinger nucleases by in vitro selection[J]. Nature Methods, 2011, 8(9): 765-770.
[16]Feng Zhang, Morgan L Maeder, Erica UngerWallace, et al. High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases[J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(26): 12028-12033.
[17]Keishi Osakabe, Yuriko Osakabe, Seiichi Toki. Sitedirected mutagenesis in Arabidopsis using customdesigned zinc finger nucleases[J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(26): 12034-12039.
[18]Shaun J Curtin, Feng Zhang, Jeffry D Sander, et al. Targeted mutagenesis of duplicated genes in soybean with zincfinger nucleases[J]. Plant Physiology, 2011,156(2): 466-473.
[19]Jens Boch, Ulla Bonas. Xanthomonas AvrBs3 familytype III effectors: discovery and function[J]. Annual Review of Phytopathology, 2010, 48: 419-436.
[20]Jens Boch, Heidi Scholze, Sebastian Schornack, et al. Breaking the code of DNA binding specificity of TALtype III effectors[J]. Science, 2009, 326(5959): 1509-1512.
[21]Adam J Bogdanove, Daniel F Voytas. TAL effectors: customizable proteins for DNA targeting[J]. Science, 2011, 333(6051): 1843-1846.
[22]Heidi Scholze, Jens Boch. TAL effectors are remote controls for gene activation[J]. Current Oopinion in Microbiology, 2011, 14(1): 47-53.
[23]Neville E Sanjana, Le Cong, Yang Zhou, et al. A transcription activatorlike effector toolbox for genome engineering[J]. Nature Protocols, 2012, 7(1): 171-192.
[24]Andrew J Wood, TeWen Lo, Bryan Zeitler, et al. Targeted genome editing across species using ZFNs and TALENs[J]. Science, 2011, 333(6040): 307.
[25]Jeffrey C Miller, Siyuan Tan, Guijuan Qiao, et al. A TALE nuclease architecture for efficient genome editing[J]. Nature Biotechnology, 2011, 29(2): 143-148.
[26]Ting Li, Sheng Huang, Xuefeng Zhao, et al. Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes[J]. Nucleic Acids Research, 2011, 39(14): 6315-6325.
[27]Peng Huang, An Xiao, Mingguo Zhou, et al. Heritable gene targeting in zebrafish using customized TALENs[J]. Nature Biotechnology, 2011,29(8): 699-700.
[28]Mahfouz M M, Li L X , Shamimuzzaman M, et al. De novoengineered transcription activatorlike effector (TALE) hybrid nuclease with novel DNA binding specificity creates doublestrand breaks[J]. Proceedings of the National Academy of Sciences of the United States of America,2011, 108(6): 2623-2628.
[29]Tomas Cermak, Erin L Doyle, Michelle Christian, et al. Efficient design and assembly of custom TALEN and other TAL effectorbased constructs for DNA targeting[J]. Nucleic Acids Research, 2011, 39(12): 1-11.
[30]Ting Li, Bo Liu, Martin H Spalding, et al. Highefficiency TALENbased gene editing produces diseaseresistant rice[J]. Nature Biotechnology, 2012, 30(5): 390-392.
[31]Magdy M Mahfouz, Lixin Li, Marek Piatek, et al. Targeted transcriptional repression using a chimeric TALESRDX repressor protein[J]. Plant Molecular Biology, 2012, 78(3): 311-321.
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