Abstract: The nuclear polyhedrosis virus of Actias selene ningpoana (ASNPV) is an insect baculovirus, which had been isolated only in china. Its genome was extracted from the virus particles of NPV by water-saturated phenol-DS. This genome was bouble-tranded DNA with 86- of Tm and 41% of G + C Content. The DNA appears as a single band in the electrophoresis of 0.7% of agarose gel. The infection experiment shows that the viral nucleic acid is biologically active.