Abstract: The diseases and pests in ecological forest in Shanghai were studied. The characteristics of diseases and pests in ecological forest were analyzed. The result showed that vegetational varieties in ecological forest in Shanghai is relatively shortage, the plantation structure is too simple, and it is lack of the natural enemy. The ecological forest easily is affected by Tropical Island Effect and artificial factor. The major factors of the pests prevalence were studied as follows:the balance of urban ecosystem was broken by increasing plantation area, exotic species were introduced unsuitable, unreasonable stand structure and extensive management existed. Which resulted in the occurence of some foreign or secondary pests’ damage to the forest. On this base, some comprehensive pests management strategies to ecological forest in Shanghai were put forward.