Abstract: MS medium was used as a basic medium. Callus induced from inflorescence axis of Euphorbia pulcherrirna was used as explants. It is compared that effects of combinations which contain different concentrations of 6-BA+ NAA and 6-BA+ IBA on characters of differentiated shoots from callus of E. pulcherrima. The results showed that, in combination of 6-BA+NAA, the best medium combination is MS+2.0 mg/L 6-BA+0.1 mg/L NAA, the subordinate is MS+1. 6 mg/L 6-BA+0.1 mg/L NAA and MS+0.8 mg/L 6-BA+0.2 mg/L NAA. While in combination of 6-BA+IBA, the best medium combination is MS+2.0 mg/L 6-BA+0.2 mg/L IBA and MS+2.0 mg/L 6-BA+0.4 mg/L IBA, the subordinate is MS+ 2.0 mg/L 6-BA+0.1 mg/L IBA.