Mitosis and chromosome number in poplar pathogenic fungus Marssonina brunnea

doi:10.3969/j.jssn.1000-2006.2012.01.005

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2012, Vol. 36 ›› Issue (01): 21-27.doi: 10.3969/j.jssn.1000-2006.2012.01.005

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Mitosis and chromosome number in poplar pathogenic fungus Marssonina brunnea

TAN Biyue¹, CAO Youzhi¹, XU Li’an¹,SU Xiaohua², ZHANG Shougong²,HUANG Minren^1*

1. College of Forest Resources and Environment, Nanjing Forestry University， Nanjing 210037, China; 2. Chinese Academy of Forestry, Beijing 100091, China

Online:2012-01-30 Published:2012-01-30

Abstract

Abstract: Mitotic division of Marssonina brunnea was analyzed by light microscopy and the germ tube burst method (GTBM). The mitosis of M. brunnea has four phases. The chromosomes gradually shortened and thickened during prophase, and distinctly visible at metaphase. In anaphase, the chromatids separated and moved to opposite poles, at last, daughter nuclei were shaped in telophase. Three kinds of dyes were utilized to stain the chromosomes of M. brunnea, they were: DAPI, DAPI/PI and Giemsa. We found that Giemsa staining performed best that chromosome number was easily counted at the metaphase stage. Chromosomes were morphologically characterized by absolute and relative sizes.

CLC Number:

Q939
S432.4

TAN Biyue, CAO Youzhi, XU Li’an,SU Xiaohua, ZHANG Shougong,HUANG Minren. Mitosis and chromosome number in poplar pathogenic fungus Marssonina brunnea[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2012, 36(01): 21-27.

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Mitosis and chromosome number in poplar pathogenic fungus Marssonina brunnea

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