JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2012, Vol. 36 ›› Issue (02): 47-51.doi: 10.3969/j.jssn.1000-2006.2012.02.010

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Characteristic analysis of microsatellites of Camellia spp.

SHI Jie, YIN Tongming, GUAN Hongwei, DAI Xiaogang, CHEN Jinhui, SHI Jisen*   

  1. Key Lab of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing 210037,China
  • Online:2012-04-10 Published:2012-04-10

Abstract: There were 11 344 microsatellites(SSRs)ranged from 1 to 6 bp detected by analyzing the DNA sequences covering about 10% of genome Camellia. Among these SSRs, dinucleotide repeats were most abundant(27.1%). For the composition of microsatellites, A/T repeats were the most frequent motifs in mononucleotide repeats, and AT/TA, AG/TC repeats were the most in dinucleotide repeats, while(AAN)n,(AAAN)n and(AAAAN)n repeats were dominant in tri-, tetra- and pentanucleotide repeats, respectively. All the dominant repeat motifs for different type of SSRs were abundant in nucleotides A and T. In Camellia genome, microsatellites longer than 20bp accounted for about 11.7% of the detected SSRs. Repeats, repeat motif lengths were found negatively correlated with the variation rate except mononucleotide repeats, thus, the shorter repeats would have faster substitution rate.

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