Abstract: The natural cellulose now has become one of the research hotspots in the water treatment research field for its excellent performance. Natural cellulose was directly dissolved in environmental-friendly alkali/carbamide/water solvent system. Reversed-phase suspension technique was used to regenerate cellulose microspheres, they were further magnetically functionalized through in-situ synthesis method. The results showed that there was a decrease in the diameters of the cellulose microspheres with an increase of the oil/water ratio. The prepared cellulose microspheres had large specific surface area(>30 m²/g), good porosity(>90%), and high water content(>85%). Based on the results of FTIR, XRD and SEM, Fe₃O₄ nanoparticles were successfully formed and loaded in the cellulose microspheres. The cellulose composites preserved good orbicular shape, porosity and superparamagnetism.This type of cellulose microspheres material possessed potential application in the water treatment field.