Isolation, antifungal effect and characteristics of non-protein substances
 produced by Bacillus pumilus JK-SX001

doi:10.3969/j.jssn.1000-2006.2012.03.004

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2012, Vol. 36 ›› Issue (03): 13-16.doi: 10.3969/j.jssn.1000-2006.2012.03.004

Previous Articles Next Articles

Isolation, antifungal effect and characteristics of non-protein substances produced by Bacillus pumilus JK-SX001

YAN Aiqin,Wu Xiaoqin,YE Jianren^*,FAN Bo

Jiangsu Key Laboratory for Prevention and Management of Invasive Species,Nanjing Forestry University,Nanjing 210037,China

Online:2012-05-30 Published:2012-05-30

Abstract

Abstract: In order to explore the effect of non-protein antifungal substances produced by Bacillus pumilus strain JK-SX001 and its mechanisms on poplar canker disease, three pathogenic fungi species Cytospora chrysosperma,Phomopsis macrospora and Fusicoccum aesculi were used. The extraction methods of the non-protein antifungal substances and its inhibitory mechanisms on mycelia and germinated-spores were studied, while we also measured some physical and chemical properties of the non-protein antifungal substances. The results showed that the non-protein antifungal substances had a strong inhibition to the mycelia growth and spore germination, and could dissolve the spore. Valid storage life had great influence on the substances. However, illumination and temperature had little effect on the activity of the antifungal substances.

CLC Number:

S763

YAN Aiqin,Wu Xiaoqin,YE Jianren,FAN Bo. Isolation, antifungal effect and characteristics of non-protein substances produced by Bacillus pumilus JK-SX001[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2012, 36(03): 13-16.

References

[1] 黄烈健,苏晓华.我国杨树溃疡病研究进展[J].世界林业科学研究,2003,16(4):49-53.
[2] 王勇,吴小芹.不同定殖处理对杨树溃疡病发生发展的影响[J].南京林业大学学报:自然科学版,2009,33(1):11-14.
[3] Mari,Guizzardi M,Pratella G C.Biological control of gray mold in pears by antagonistic bacteria[J].Biological Control,1996,7(1):30-37.
[4] 杨文博,费成,金星.拮抗微生物的开发与利用[J].世界农业,1998,21(8):28-31.
[5] 杨冬静,王建新,周明国.Bacillus subtilis NJ-18菌株对番茄早疫病菌的拮抗作用研究[J].微生物学通报,2009,36(8):1166-1169
[6] 于婷,尚玉珂,李艳芳,等.短小芽孢杆菌BSH-4抗菌物质的提取及其特性[J].植物保护学报,2009,36(1):66-69.
[7] 任嘉红.杨树溃疡病拮抗细菌的筛选及拮抗物质的研究[D].南京:南京林业大学,2009.
[8] 王勇,吴小芹.苏北杨树溃疡病类型及其病原的致病力[J].南京林业大学学报:自然科学版,2008,32(5):47-50.
[9] 何红,蔡学清,关雄,等.内生菌BS-2菌株的抗菌蛋白及其防病作用[J].植物病理学报,2003,33(4):373-378.
[10] 陈祥贵,裴炎.蜡状芽孢杆菌S-1菌株抗真菌物质的分离纯化[J].四川工业学院学报,2002,21(2):60-62.
[11] 童有仁,马志超,陈卫良,等.枯草芽孢杆菌B034拮抗蛋白的分离纯化及特性分析[J].微生物学报,1999,39(4):339-343.
[12] 陈志谊,高太东,严大富,等.枯草芽孢杆菌B-916防治水稻纹枯病的田间试验[J].中国生物防治,1997,13(2):75-78.
[13] 何亮.蜡状芽孢杆菌C332的鉴定、发酵及其抗菌物质的分离纯化与性质研究[D].杨凌:西北农林科技大学,2007.
[14] 姜俏,林琳,汪天虹.丝状真菌细胞凋亡研究进展[J].微生物学报,2008,48(4):551-555.
[15] 孔建,王文夕,赵白鸽,等.枯草芽孢杆菌B-903菌株的研究.Ⅲ:影响抗菌物质产生积累的主要因素[J].中国生物防治,2000,6(2):65-69.
[16] 沈娟,陆兆新,别小妹,等.Bacillus sp.fmbj224发酵产新型抗菌肽培养基的优化研究[J].生物工程学报,2005,21(4):609-614.
[17] 徐广军.枯草芽孢杆菌BS3-1对小麦赤枯病的生物防治研究[D].成都:四川农业大学,2003.
[18] 田黎,刘晓光,高克祥,等.拮抗枯草芽孢杆菌在生物防治中的应用与研究进展[J].中国生物防治,2002,18(4):180-184.
[19] Asaka O, Shoda M. Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subrilis RB-14[J].Applide and Environmental Microbiology,1996,62:4081-4085.
[20] PEI T, REN D,SHI J.Study on purification and properties of antibacterial substance from antagonistic bacteria P72 against wheat scab[J].Agricultural Science and Technology,2008,9(6):124-126.

Related Articles 15

[1]	WANG Lichao, CHEN Fengmao, DONG Xiaoyan, SHENG Ruocheng, LI Huan, WANG Mengyao. Identification of a newly recorded species of Aphelenchoides paradanlianensis in China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2025, 49(2): 169-174.
[2]	LI Zichun, HAO Dejun, LI Hui, LI Changyan, XU Danwenyi, YANG Hualei, ZHAO Peiyuan. Cloning of glutathione S-transferases gene from Monochamus alternatus and its expression characteristics under heat stress [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2025, 49(1): 28-36.
[3]	CHEN Youmei, XIA Xinrui, YE Jianren, ZHU Lihua. An in vitro evaluation of the resistance traits to pine wood nematode (Bursaphelenchus xylophilus) in Pinus massoniana embryogenic callus [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2025, 49(1): 37-45.
[4]	WANG Junwei, HU Longjiao, WU Xiaoqin. Comparison of pathogenicity and reproduction of Bursaphelenchus xylophilus in pine families with different disease resistance [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2025, 49(1): 21-27.
[5]	YAN Xiongfei, YANG Liuji, LIU Yonghua, LI Gang, FENG Jiangshuai. Analyzing ultrastructure of Pcythropus yasumatsui (Coleoptera: Curculionida) antennal sensilla by the scanning electron microscope [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(6): 245-251.
[6]	XUE Mingyu, HAO Dejun, ZHAO Xudong, GENG Yishu, HU Tianyi, XIE Chunxia. Effects of extreme climate on the distribution and potential habitat of Hyphantria cunea in China [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(5): 197-203.
[7]	XIE Jiaming, CAO Chuanwang, SUN Lili, LI Mingjun, ZHANG Ruiqiong. Structural prediction of glutathione S-transferase (GST) in Lymantria dispar and its molecular docking analysis with poplar secondary metabolites [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(5): 211-220.
[8]	PENG Mengmeng, WU Hongqu, ZHANG Jiawen, YAN Liqiong, CAO Chuanwang, SUN Lili. HcAnk1 and HcAnk2 genes function and HcNPV susceptibility of Hyphantria cunea based on RNAi [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 181-190.
[9]	FANG Jing, ZHANG Shuman, YAN Shanchun, WU Shuai, ZHAO Jiaqi, MENG Zhaojun. Effects of the compound inoculation of two arbuscular mycorrhizal(AM) fungi on the resistance of Populus pseudo-cathayana × P. deltoides leaves to Hyphantria cunea [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 144-154.
[10]	ZHU Yanyan, JIA Ruirui, FU Yu, CHANG Lin, YUE Yuanzheng, YANG Xiulian, WANG Lianggui. Differences in resistance of Catalpa bungei cultivars to stem rot [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 155-165.
[11]	ZHANG Xinfang, WANG Guangpeng, ZHANG Shuhang, LI Ying, GUO Yan. Screening and analysis of differential secondary metabolites in Castanea mollissima with different levels of resistance to Oligonychus ununguis [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 234-240.
[12]	ZHAO Ya’nan, SUN Tianhua, WANG Lifeng, XU Qiang, LIU Junxia, GAO Baojia, ZHOU Guona. Plant hormones and metabolites response to feeding stimulation by pine caterpillar (Dendrolimus tabulaeformis) and leaf clipping control in Chinese pine (Pinus tabuliformis) [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 219-226.
[13]	ZHANG Chenghui, ZU Guohao, WANG Haiyang, XUE Hao. A new record species of Exoristobia from China (Hymenoptera: Encyrtidae) [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 214-218.
[14]	SUN Kaili, HE Chunling, HU Junjie, FAN Quanbo, LUAN Ke, REN Yingfeng, XIAO Zhishu. Study on parasitic behavior of Pseudoxenos iwatai in the abdomen of Rhynchium quinquecinctum [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 243-250.
[15]	CHENG Fang, SUN Tingyu, YE Jianren. Induction of embryogenic callus from immature zygotic embryos of Pinus elliottii resistant to brown spot needle blight (pathogen: Lecanosticta acicola) [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(6): 175-182.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

Isolation, antifungal effect and characteristics of non-protein substances produced by Bacillus pumilus JK-SX001

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer