Abstract: In order to discuss the effects of land use change along the road in mountains region on the ecosystem service value, we took provincial road 309 in Majiang county in Guizhou Province as an example, and analyzed the change of ecosystem service value along the road resulting from land use change based on land use data 1992, 2002, 2012 and 2032 and ecosystem service value coefficient per unit area in China. The results showed that: ① farmland and grassland were consistently decreasing, construction land would keep increasing, while forest land, water body and unused land underwent a positive or negative change during the study period. ② Ecosystem services value for scenario A and C increased except for scenario B. The variation trend of each ecosystem services value was in accordance with the change trend of each land use type. Each category of ecosystem services value continued to increase for scenario A and C except for scenario B. The change rate of ecosystem service value in 0-500 m buffer was bigger than that in the other buffers. In addition, there were differences between different periods for the change of ecosystem service value in four buffers. ③ Rapid economic development, grain for green policy and terrain were the main factors influencing the change of ecosystem service value along the road in mountains region.