Influence of nitrogen levels on photosynthesis,nutrient elements and 
camptothecin content of Camptotheca acuminata

doi:10.3969/j.issn.1000-2006.2016.03.003

Influence of nitrogen levels on photosynthesis,nutrient elements and camptothecin content of Camptotheca acuminata

KUANG Heling, WANG Guibin,CAO Fuliang

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (03) : 15-20.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (03) : 15-20. DOI: 10.3969/j.issn.1000-2006.2016.03.003

Influence of nitrogen levels on photosynthesis,nutrient elements and camptothecin content of Camptotheca acuminata

KUANG Heling, WANG Guibin^*,CAO Fuliang

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A field experiment was carried out to investigate the effect of different nitrogen on growth of Camptotheca acuminata seedlings, photosynthesis, leaf nutrient elements and camptothecin content by determine the variation of photosynthetic rate, chlorophyll content, camptothecin content and so on. The results indicated that with the increase of nitrogen level, the photosynthesis, phosphorus and camptothecin content in C. acuminata leaves initially increased then decreased. Photosynthetic rate and phosphorus element content were the highest in the fertilizering amount of 30 g per plant, and camptothecin content was the highest in the fertilizering amount of 20 g per plant. Chlorophyll and nitrogen content, along with the increased nitrogen levels showed a gradual upward trend, and they were the highest in the fertilizering amount of 50 g per plant. Potassium content with the increasing nitrogen level gradually decreased, it was the lowest in the fertilizering amount of 50 g per plant. Camptothecin yield per plant in the fertilizering amount of 30 g treatment was the highest, the control(N0)was the lowest. Based on a comprehensive analysis of all indicators, we suggested that the right amount of nitrogen levels(30 g per plant)would improve the C. acuminata photosynthetic rate and promote the accumulation of nutrient elements in the leaf, thus contributing to the biosynthesis of camptothecin.

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KUANG Heling, WANG Guibin,CAO Fuliang. Influence of nitrogen levels on photosynthesis,nutrient elements and camptothecin content of Camptotheca acuminata[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2016, 40(03): 15-20 https://doi.org/10.3969/j.issn.1000-2006.2016.03.003

References

[1] 刘勇, 陈燕, 张志毅, 等. 不同施肥处理对三倍体毛白杨苗木生长及抗寒性的影响[J]. 北京林业大学学报, 2000,22(1):38-44. Doi:10.13332/j.1000-1522.2000.01.010. Liu Y, Chen Y, Zhang Z Y, et al. Effect of fertilizer treatments on seedling growth and cold resistance of triploid Populus tomentosa[J]. Journal of Beijing Forest University, 2000,22(1):38-44.
[2] Albaugh T J, Allen H L, Dogherty P M, et al. Long term growth responses of loblolly pine to optimal nutrient and water resource availability[J]. Forest Ecology and Management, 2004,192:3-19. Doi:10.1016/j.foreco.2004.01.002.
[3] Jeyanny V, Abrasip A G, Rasidah K, et al. Effects of macronutrient deficiencies on the growth and vigour of Khaya ivorensis seedlings[J]. Journal of Tropical Forest Science, 2009,21(2):73-80.
[4] 梁宏温, 黄承标, 梁杏清, 等. 不同施肥处理马尾松幼林生长及其营养元素含量的研究[J]. 广西农业大学学报, 1996,15(4):309-314. Liang H W, Huang C B, Liang X Q, et al.Different fertilizer treatments Pinus massoniana Lamb. young forest growth and nutrient elements content of research[J]. Journal of Guangxi Agricultural University, 1996,15(4):309-314.
[5] 孙曦. 植物营养原理[M]. 北京: 中国农业出版社, 1997.
[6] Bondada B R, Osterhuis D M, Norman R J. Canopy photosynthesis, growth, yield and boll¹⁵N accumulation under nitrogen stress in cotton[J]. Crop Science, 1996,36:127-133. Doi:10.2135/cropsci1996.0011183X003600010023x
[7] 张旺锋,勾玲, 王振林, 等. 氮肥对新疆高产棉花叶片叶绿素荧光动力学参数的影响[J]. 中国农业科学, 2003,36(8):893-898. Doi:10.3321/j.issn:0578-1752.2003.08.006. Zhang W, Gou L, Wang Z L, et al. Effect of nitrogenon chlorophyll fluorescence of leaves of high yielding cotton in Xinjiang[J]. Scientia Agricultura Sinica, 2003,36(8):893-898.
[8] Cechin I, Terezinha F F. Effect of nitrogen supply on growth and photosynthesis of sunflower plants grown in the greenhouse[J]. Plant Science, 2004,166(5):1379-1385. Doi:10.1016/j.plantsci.2004.01.020.
[9] Misra N, Gupta A K. Effect of salinity and different nitrogen sources on the activity of antioxidant enzymes and indole alkaloid content in Catharanthus roseus seedlings[J]. J Plant Physiol, 2006,163(1):11. Doi:10.1016/j.jplph.2005.02.011.
[10] 杜永成, 王玉波, 范文婷, 等. 不同氮素水平对甜菜硝酸还原酶和亚硝酸还原酶活性的影响[J]. 植物营养与肥料学报, 2012,18(3):717-723. Doi:10.11674/zwyf.2012.11331. Du Y C, Wang Y B, Fan W T, et al. Effect of nitrogen fertilization on nitrate reductase and nitrite reductase activities of sugar beet[J]. Journal of Plant Nutrition and Fertilizer, 2012, 18(3):717-723.
[11] Rothstein D E, Zak D R, Pregitzer K S, et al. Kinetics of nitrogen uptake by Populus tremuloides in relation to atmospheric CO₂ and soil nitrogen availability[J]. Tree Physiology, 2000,20(4):265-270. Doi:10.1093/treephys/20.4.265.
[12] 王庆成, 程云环. 土壤养分空间异质性与植物根系的觅食反应[J]. 应用生态学报, 2004,15(6):1063-1068. Doi:10.3321/j.issn:1001-9332.2004.06.030. Wang Q C, Cheng Y H. Response of fine roots to soil nutrient spatial heterogeneity[J]. Chinese Journal of Applied Ecology, 2004,15(6):1063-1068.
[13] 谷尘平, 姜高明. 水杉苗期对氮素营养需求的研究[J]. 湖北林业科技, 2004(2):19-22. Doi:10.3969/j.issn.1004-3020.2004.02.005. Gu C P, Jiang G M.Study on requirement for nitrogen nutrition by seedling of Metasequoia glyptostroboides[J]. Hubei Forestry Science and technology, 2004(2):19-22.
[14] 范志强, 王政权, 吴楚, 等. 不同供氮水平对水曲柳苗木生物量、氮分配及其季节变化的影响[J]. 应用生态学报, 2004,15(9):1497-1501. Doi:10.3321/j.issn:1001-9332.2004.09.002. Fan Z Q, Wang Z Q, Wu C, et al.Effect of different nitrogen supply on Fraxinus mandshurica seedlings biomass, N partitioning and their seasonal variation[J]. Chinese Journal of Applied Ecology, 2004,15(9):1497-1501.
[15] 白尚斌, 王懿祥. 北美红杉幼苗对不同供N水平的生长反应[J]. 应用生态学报, 2005,18(5):561-566. Doi:10.13275/j.cnki.lykxyj.2005.05.011. Bai S B, Wang Y X.Growth response of Sequoia sempervirens seedlings to N nutrients[J]. Chinese Journal of Applied Ecology, 2005,18(5):561-566.
[16] 《四川森林》编辑委员会. 四川森林[M]. 北京: 中国林业出版社, 1992.
[17] 郑晓龙. 抗肿瘤药物喜树碱的研究进展[J]. 中国现代药物应用, 2014(19):245-246. Zheng X L.Research progress of antineoplastic agents camptothecin[J]. Chinese Journal of Modern Drug Application, 2014(19):245-246.
[18] 冯建灿, 张玉洁, 谭运德, 等. 喜树与喜树碱开发利用进展[J]. 林业科学, 2000,36(5):100-108. Doi:10.3321/j.issn:1001-7488.2000.05.017. Feng J C, Zhang Y J, Tan Y D, et al. The development in research on Camptotheca acuminata and utilization of camptothecin[J]. Scientia Silvae Sinicae, 2000,36(5):100-108.
[19] 张志良, 翟伟箐. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2003:35-43. Zhang Z L, Zhang W Q.Plant physiology experimental guidance[M]. Beijing: Higher Education Press, 2003:35-43.
[20] 贝美容,罗雪华,杨红竹.AA3型连续流动分析仪(CFA)同时测定橡胶叶全氮、全磷、全钾的方法研究[J].热带作物学报,2011,32(7):1258-1264.Doi:10.3969/j.issn.1000-2561.2011.07.015.
[21] 梁冬丽,梁宏合.微波消解-火焰光度法测定植物中全钾的研究[J].广西热带农业,2010,129(4):24-26.
[22] 孙世芹, 阎秀峰. 氮素水平对喜树幼苗喜树碱含量的影响[J]. 中国中药杂志, 2003,8(2):356-359. Doi:10.3321/j.issn:1001-5302.2008.04.003. Sun S Q, Yan X F.Effect of nitrogen on camptothecin content in Camptotheca acuminata seedlings[J]. China Journal of Chinese Materia Medica, 2003,8(2):356-359.
[23] 贾瑞丰, 尹光天, 杨锦昌, 等. 不同氮素水平对红厚壳幼苗生长及光合特性的影响[J]. 林业科学研究, 2012,25(1):23-29. Doi:10.13275/j.cnki.lykxyj.2012.01.013. Jia F R, Yin G T, Yang J C, et al.Effects of nitrogen application on the growth and photosynthetic characteristics of Calophyllum inophyllum seedlings[J]. Forest Research, 2012,25(1):23-29.
[24] 陈静, 刘连涛, 孙红春, 等. 氮素水平对棉花幼苗生长和光合特性的影响[J]. 棉花学报, 2013,25(5): 403-409. Doi:10.3969/j.issn.1002-7807.2013.05.005. Chen J, Liu L T, Sun H C, et al.Effects of nitrogen concentrations on cotton seedling growth and photosynthetic characteristics[J]. Cotton Science, 2013,25(5): 403-409.
[25] Nakaji T, Takenaga S, Kuroha M, et al. Photosynthetic response of Pinus densiflora seedlings to high nitrogen load[J]. Environmental Sciences, 2002,9(4):269-282.
[26] 蔡永萍, 陶汉之, 程备久. 对生玉米叶片蒸腾、光合若干特性的研究[J]. 安徽农业大学学报, 1996, 23(4):474-477. Doi:10.13610/j.cnki.1672-352x.1996.04.006. Cai Y P, Tao H Z, Cheng B J. Opposite maize leaf transpiration and photosynthetic research of several characteristics[J]. Journal of Anhui Agricultural University, 1996, 23(4):474-477.
[27] 武志海, 杨美英, 吴春胜, 等. 玉米群体冠层内蒸腾速率与气孔导度的变化特性[J]. 吉林农业大学学报, 2001,23(4):18-20. Doi:10.13327/j.jjlau.2001.04.005. Wu Z H, Yang M Y, Wu C S, et al.Varying characteristics of transpirationrate and stomata conductance in maize canopies[J]. Journal of Jilin Agricultural University, 2001,23(4):18-20.
[28] 邹振华, 党宁, 王惠群, 等. 不同氮素水平对营养生长期南荻植株光合特性的影响[J]. 作物研究, 2012,26(3):255-258. Doi:10.3969/j.issn.1001-5280.2012.03.12. Zou Z H, Dang N, Wang H Q, et al.Effects of different nitrogen rates on photosynthetic characteristics in vegetative period of Triarrhena lutarioriparia L. Liu[J]. Crop Research, 2012,26(3):255-258.
[29] 肖丽, 高瑞凤, 隋方功. 氯胁迫对大白菜幼苗叶绿素含量及光合作用的影响[J]. 中国土壤与肥料, 2008(2):44-47. Xiao L, Gao R F, Sui F G.Effects of chloride stress on the photosynethesis and chlorophyll content of chinese cabbage seedlings[J]. Soils and Fertilizer Sciences in China, 2008(2):44-47.
[30] Attipalli R R, Kolluru V C, Munusamy V. Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants[J]. Plant Physiology, 2004,161:1189-1202. Doi:10.1016/j.jplph.2004.01.013.
[31] Allakhverdiev S I, Sakamoto A, Nishiyama Y, et al. Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.[J]. Plant Physiology, 2000,123:1047-1056.
[32] 张永江. 植物叶绿素荧光被动遥感探测及应用研究[D]. 杭州:浙江大学, 2006:87-90. Zhang Y J. Studies on passive sensing of plant chorophyll fluorescence and application of stress detection[D]. Hangzhou: Zhejiang University, 2006:87-90.
[33] 申玉香, 栾文婷. 氮素水平对小麦幼苗性状和营养元素含量的影响[J]. 中国农学通报, 2009,25(24): 196-199. Shen Y X, Luan W T.Effect of different nitrogen levels on wheat seedling trait and nutrient content[J]. Chinese Agricultural Science Bulletin, 2009,25(24): 196-199.
[34] Liu Z, Adams J C, Viator H P, et al. Influence of soil fertilization, plant spacing, and coppicing on growth, stomatal conductance, abscisic acid, and camptotheca in levels in Camptotheca acuminata seedlings[J]. Physiol Plantarum,1999,105:402. Doi:10.1034/j.1399-3054.1999.105303.x.
[35] 邢军会. 氮素营养对喜树幼苗生长及其叶片喜树碱含量的影响[D]. 哈尔滨:东北林业大学, 2003:42-43. Xing J H.Effects of nitrogen nutrition on growth and camptothecin concentration in leaves of Camptotheca acuminate seedlings[D]. Harbin: Northeast Forestry University, 2003:42-43.
[36] 冯立新, 苏付保, 黎健杏, 等. 密度与施肥对半年生喜树苗喜树碱含量的影响[J]. 福建林业科技, 2014, 41(3):40-43. Doi:10.13428/j.cnki.fjlk.2014.03.009. Feng L X, Su F B, Li J X, et al.Density and fertilization influence on the leaves camptothecin content of C.acuminata half year old seedling[J]. Jour of Fujian Forestry Sci and Tech, 2014, 41(3):40-43.

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