Effects of temperature on the growth and camptothecin contents
 of Camptotheca acuminata

doi:10.3969/j.issn.1000-2006.2016.03.004

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (03): 21-25.doi: 10.3969/j.issn.1000-2006.2016.03.004

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Effects of temperature on the growth and camptothecin contents of Camptotheca acuminata

WANG Guibin¹, ZHOU Xin¹, LIU Lin¹, CAO Fuliang¹, ZU Yuangang²

1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. Key Laboratory of Forest Plant Ecology Ministry of Education, College of Forestry, Northeast Forestry University, Harbin 150040, China

Online:2016-06-18 Published:2016-06-18

Abstract

Abstract: Camptothecin(CPT)is an important secondary metabolite in Camptotheca acuminata, and it can be used as an anticancer drug. The biosynthesis and accumulation of CPT in C. acuminata are affected by heredity and environment. In this paper, the effect of temperature on the growth and CPT biosynthesis of one year old C. acuminata was studied in phytotron. Three temperature day/night(day time 6:00-18:00,night time18:00-6:00), 30 ℃/25 ℃(T1), 35 ℃/30 ℃(T2)and 40 ℃/30 ℃(T3)were designed. We found that C. acuminata presented the highest biomass at T3, and T2 was beneficial to the height and caliper of seedlings, and T3 was favorable to the accumulation of stem and root biomass. T2 treatment presented the highest soluble sugar content, but the lowest soluble protein content and the activity of tryptophan decarboxylas(TDC). At T2 condition, the highest CPT content and CPT yield per plant in the leaves were observed. The changing regulation of CPT content was not in accordance with the activity of TDC. So appropriate temperature T2 is very important to the biosynthesis and accumulation of CPT, and the biosynthesis and accumulation of CPT is a comprehensive result of enzymes and environmental factors.

CLC Number:

S718
S792.99

WANG Guibin, ZHOU Xin, LIU Lin, CAO Fuliang, ZU Yuangang. Effects of temperature on the growth and camptothecin contents of Camptotheca acuminata[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2016, 40(03): 21-25.

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Effects of temperature on the growth and camptothecin contents of Camptotheca acuminata

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