Micromorphological characters of leaf epidermis in Magnolia sieboldii from different habitats

ZHANG Shuyuan, DU Fengguo, WANG Ruijian, WANG Jinling, SUN Guangren, GAO Hongbing

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2016, Vol. 40 ›› Issue (04) : 56-62.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2016, Vol. 40 ›› Issue (04) : 56-62. DOI: 10.3969/j.issn.1000-2006.2016.04.009

Micromorphological characters of leaf epidermis in Magnolia sieboldii from different habitats

  • ZHANG Shuyuan, DU Fengguo*, WANG Ruijian, WANG Jinling, SUN Guangren, GAO Hongbing
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Abstract

Magnolia sieboldii is an endangered, rare woody plant species, with a discontinuous natural distribution and high values in perfume, pharmaceutical, food and ornamental industries. To explore genetic variations within-species and relationships among different habitats, we used scanning electronic microscope, principal component analysis, and cluster analysis to quantify the micromorphological characters of leaf epidermis among M. sieboldii populations from seven habitats. The results showed that there were two types of epidermal cells(shallow wavy and deep wavy), two types of stomatal apparatuses(elliptically convex and concave), two types of leaf epidermis crystals(spherical and cylindrical), three types of dense epidermal hairs(unicellular, single-row bicellular and single-row multicellular), as well as variations in stomata size, density and epidermal hair length and density. The principal component analysis identified three principal components accounted for 87.394% of the total variation in micromorphological characters of leaf epidermis. The cluster analysis showed that M. sieboldii from seven habitats belonged to two groups. The first group included the M.sieboldii from Shihu in Jilin Province and Zushan in Hebei Province, and this group had shallow wavy leaf epidermal cells, lower stomatal density, convex stomatal apparatus, short stomatal axis(<10 μm)and round leaf crystals. The second group included the M.sieboldii from Qingliangfeng in Zhejiang Province, Huangshan in Anhui Province, Leigong Mountain and Majiang in Guizhou Province and Maoershan in Guangxi Province. This group generally had deep wavy leaf epidermal cells, higher stomatal density, concave stomata, long stomatal axis(>10 μm)and long cylindrical leaf crystals. These results suggested that the genetic relationships among different habitat in M. sieboldii were related to the geographical distribution of the species and that the micromorphological characters of leaf epidermis could be used to study genetic variations and relationships among different habitat in M. sieboldii.

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ZHANG Shuyuan, DU Fengguo, WANG Ruijian, WANG Jinling, SUN Guangren, GAO Hongbing. Micromorphological characters of leaf epidermis in Magnolia sieboldii from different habitats[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2016, 40(04): 56-62 https://doi.org/10.3969/j.issn.1000-2006.2016.04.009

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