Effects of two phosphate-solubilizing Penicillium strains on microbial community structure and their growth-promoting in masson pine rhizosphere

QIAO Huan, WU Xiaoqin, WEI Wei

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (06) : 109-116.

PDF(2187528 KB)
南京林业大学学报（自然科学版）
PDF(2187528 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2016, Vol. 40 ›› Issue (06) : 109-116. DOI: 10.3969/j.issn.1000-2006.2016.06.017

Effects of two phosphate-solubilizing Penicillium strains on microbial community structure and their growth-promoting in masson pine rhizosphere

  • QIAO Huan, WU Xiaoqin*, WEI Wei
Author information +
History +

Abstract

Two fungal strains Penicillium guanacastense JP-NJ2 and P. pinophilum JP-NJ4 which had strong capability of dissolving phosphate was isolated from Pinus massoniana rhizosphere early. The effects of the strains on microbial community structure and growth-promoting in masson pine rhizosphere were studied. Microbial community structure in masson pine rhizosphere and growth-promoting of the pines were measured with the Biolog method and then the effects of two phosphate-solubilizing fungi(PSF)were analyzed. The impact of two strains on growth of masson pine was observed. The applications of PSF P. guanacastense JP-NJ2 and P. pinophilum JP-NJ4 and their metabolite significantly changed the species dominance and richness index both in winter and summer. The two PSF raised the number of fungi in the soil and reached the largest biomass in summer(59.27×104 cfu/g). In summer, species richness indexes treated by the JP-NJ2 suspension and JP-NJ4 suspension were 2.835 0 and 3.120 0, respectively. Both of the species richness indexes of two fungal suspensions were lower than that of the control. This indicated that soil species richness reduced in summer. The application of JP-NJ2 and JP-NJ4 in masson pine rhizosphere significantly promoted pine growth after inoculation 600 days. The height of masson pine seedlings treated with fungal suspension increased 88.7% and root crown diameter incremented 53.5%.The applications of P. guanacastense JP-NJ2 and P. pinophilum JP-NJ4 were found greatly to alter the number of bacteria, fungi and actinomycetes as well as the total number of microorganisms. Both of two strains could promote masson pine growth well and the effect of P. guanacastense JP-NJ2 suspension was the best. The results of this research showed an important indicative reference to develop phosphate-solubilizing microbiological fertilizer.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
QIAO Huan, WU Xiaoqin, WEI Wei. Effects of two phosphate-solubilizing Penicillium strains on microbial community structure and their growth-promoting in masson pine rhizosphere[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2016, 40(06): 109-116 https://doi.org/10.3969/j.issn.1000-2006.2016.06.017

References

[1] 刘文干. 三株红壤高效溶磷菌的分离、鉴定、溶磷特性及对花生促生效应的研究[D]. 南京: 南京农业大学, 2012. Liu W G. Study on screening, identification, characteristic of phosphate solubilizing and promotion effect of three phosphate solubilizingmicroorganism from red soil[D]. Nanjing: Nanjing Agricultural University, 2012.
[2] 吴才武, 赵兰坡. 土壤微生物多样性的研究方法[J]. 中国农学通报, 2011, 27(11): 231-235. Wu C W, Zhao L P. Technologies on soil microbiology diversity[J]. Chinese Agricultural Science Bulletin, 2011, 27(11): 231-235.
[3] 冯月红, 姚拓, 龙瑞军. 土壤解磷菌研究进展[J]. 草原与草坪, 2003, 23(1): 3-7.Doi:10.3969/j.issn.1009-5500.2003.01.001. Feng Y H, Yao T, Long R J.Research progress of phosphate-dissolving microorganisms in plant rhizosphere[J]. Grassland and Turf, 2003, 23(1): 3-7.
[4] 金术超, 杜春梅, 平文祥, 等. 解磷微生物的研究进展[J]. 微生物学杂志, 2006, 26(2): 73-78. Jin S C, Du C M, Ping W X, et al. Advance in phosphorus-dissolving microbes[J]. Journal of Microbiology, 2006, 26(2): 73-78.
[5] 骆宗诗, 温佐吾. 马尾松次生林研究现状和展望[J]. 贵州林业科技, 2004, 32(3): 47-55. Luo Z S, Wen Z W.The status and prospects in research of secondary masson pine forest[J]. Guizhou Forestry Science and Technology, 2004, 32(3): 47-55.
[6] 黄鹏飞,刘君昂,靳爱仙, 等. 马尾松根际土壤溶磷菌分离筛选、鉴定及其溶磷效果研究[J]. 中国农学通报, 2012, 28(19): 12-16.Doi:10.3969/j.issn.1000-6850.2012.19.003. Huang P F, Liu J A, Jin A X, et al. Isolation and Screening, Identification of phosphorus-solubilizing bacteria in rhizosphere soil of Pinus massoniana and its phosphate-degradation capacity[J]. Chinese Agricultural Science Bulletin, 2012, 28(19): 12-16.
[7] 吴庆梅, 黄宝灵, 吕成群, 等. 不同松林根际促生菌的数量研究[J]. 广西农业科学, 2009, 40(5): 521-526.Doi:10.3969/j.issn.2095-119/2009.05.018. Wu Q M, Huang B L, Lv C Q, et al. Population of growth-promoting rhizobacteria(GPR)in different pine forests[J]. Guangxi Agricultural Sciences, 2009, 40(5): 521-526.
[8] Sylvia D M, Fuhmann J J. Princiles and applications of soil microbiology[J]. New Age International, 2005,31(2):11-68.
[9] Peix A, Rivas-Boyero A A, Mateos P F, et al. Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions[J]. Soil Biology and Biochemistry, 2001, 33(1): 103-110.Doi:10.1016/S0038-0717(00)00120-6.
[10] Zaidi A, Khan M S, Amil M D. Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea(Cicer arietinum L.)[J]. European Journal of Agronomy, 2003, 19(1): 15-21.Doi:10.1016/S1161-0301(02)0015-1.
[11] 刘志明, 余玉冰, 秦碧霞, 等. 根结线虫幼虫致病细菌的筛选[J]. 中国生物防治, 2000, 16(3): 142.Doi:10.3321/j.issn.005-9261.2000.03.011 Liu Z M, Yu Y B, Qin B X, et al. Screening of the pathogenic bacteria of root knot nematode larva[J]. Chinese Journal of Biological Control, 2000, 16(3): 142.
[12] 郜春花, 王岗, 董云中, 等. 解磷菌剂盆栽及大田施用效果[J]. 山西农业科学, 2003, 31(3): 40-43.Doi:10.3969/j.issn.1002-2481.2003.03.010. Hao C H, Wang G, Dong Y Z, et al. Effect of phosphorus bacteria in the pot and the field[J]. Journal of Shanxi Agricultural Sciences, 2003, 31(3): 40-43.
[13] 吴鹏飞, 张冬明, 郝丽虹, 等. 解磷微生物研究现状及展望[J]. 中国农业科技导报, 2008, 10(3): 40-46.Doi:10.3969/j.issn.1008-0864.2008.03.007. Wu P F, Zhang D M, Hao L H, et al. Status quo and prospects of phosphate-soluble microorganisms[J]. Journal of Agricultural Science and Technology, 2008, 10(3): 40-46.
[14] 魏伟. 马尾松根际高效解磷真菌的筛选鉴定及其促生效应[D]. 南京: 南京林业大学, 2012. Wei W.Screening and identification of masson pine rhizosphere phosphate-solubilizing fungi and its application[D]. Nanjing: Nanjing Forestry University, 2012.
[15] 魏伟, 吴小芹, 乔欢. 马尾松根际高效解磷真菌的筛选鉴定及其促生效应[J]. 林业科学, 2014, 50(9): 82-88.Doi:10.11707/j.1001-7488.20140911. Wei W, Wu X Q, Qiao H. Screening and identification of phosphate-solubilizing fungi of Pinus massoniana rhizosphere and its application[J]. Scientia Silvae Sinicae, 2014, 50(9): 82-88.
[16] 乔欢, 吴小芹, 王早. 一株嗜松青霉JP-NJ4的解磷特性[J]. 微生物学通报, 2014, 41(9): 1741-1748.Doi:10.13344/j.microbiol.china.130828. Qiao H, Wu X Q, Wang Z. Phosphate-solubilizing characteristic of a Penicillium pinophilum strain JP-NJ4[J]. Microbiology China, 2014, 41(9): 1741-1748.
[17] 林先贵. 土壤微生物研究原理与方法[M]. 北京: 高等教育出版社, 2010. Lin X G. Principles and methods of soil microbiology research[M]. Beijing: Higher Education Press, 2010.
[18] 周娟. 山东省典型农产品基地土壤微生物多样性与地球化学元素关系研究[D]. 济南: 山东大学, 2008. Zhou J.Relationships between soil microbial diversity and geochemical elements in typical agricultural products bases in Shandong Province, China[D]. Ji'nan: Shandong University, 2008.
[19] 刘子雄, 朱天辉, 张健. 林木根系分泌物与根际微生物研究进展[J]. 世界林业研究, 2005, 18(6): 25-31.Doi:10.3969/j.issn.1001-424/2005.06.004. Liu Z X, Zhu T H, Zhang J. Research advances in root exudates and rhizosphere microorganisms of forest trees[J].World Forestry Research, 2005, 18(6): 25-31.
[20] Garland J L. Analysis and interpretation of community-level physiological profiles in microbial ecology[J]. FEMS Microbiology Ecology, 1997, 24(4): 289-300.Doi:10.1016/S0168-6496(97)00061-5.
[21] 高悦, 吴小芹, 孙民琴. 马尾松不同菌根苗对氮磷钾的吸收利用[J]. 南京林业大学学报(自然科学版), 2009, 33(4): 77-80.Doi:10.3969/j.issn.1000-2006.2009.04.016. Gao Y, Wu X Q, Sun M Q. Effects of ectomycorrhizal seedlings of masson pine on absorption and utilization of N, P and K[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2009, 33(4): 77-80.
[22] 刘俊杰. 磷浓度对大豆根际微生物群落结构及功能的影响[D]. 哈尔滨: 东北农业大学, 2009. Liu J J. Effects of different phosphorous concentration on microbial community structure and function in soybean rhizosphere[D]. Harbing: Northeast Agricultural University, 2009.
[23] Oberson A, Fardeau J C, Besson J M, et al. Soil phosphorus dynamics in cropping systems managed according to conventional and biological agriculturl methods[J]. Biology and Fertility of Solis, 1993, 16(2): 111-117.Doi:10.1007/bf00369411.
[24] 钟传青, 黄为一. 磷细菌 P17 对不同来源磷矿粉的溶磷作用及机制[J]. 土壤学报, 2004, 41(6): 931-937.Doi:10.3321/j.issn:0564-3929.2004.06.014. Zhong C Q, Huang W Y. Effects and mechanism of P-solubilizing Bacillus sp. 17 strain on phosphorus solubilization of different phosphate rocks[J]. Acta Pedologica Sinica, 2004, 41(6): 931-937.
[25] Cakmakci R, Donmez F, Aydin A, et al. Growth promotion of plants by plant growth-promoting rhizobacteria under greenhouse and two different field soil conditions[J]. Soil Biology and Biochemistry, 2006, 38(6): 1482-1487.Doi:10.1016/j.soilbia.2005.09.019.
PDF(2187528 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉