Nitrogen form and ratio affect the carbon-nitrogen metabolism of Ginkgo biloba

doi:10.3969/j.issn.1000-2006.201807043

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (02): 197-202.doi: 10.3969/j.issn.1000-2006.201807043

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Nitrogen form and ratio affect the carbon-nitrogen metabolism of Ginkgo biloba

HUANG Dongjing, CAO Fuliang, WANG Guibin^*

(Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry,Nanjing Forestry University, Nanjing 210037, China)

Online:2019-03-30 Published:2019-03-30

Abstract

Abstract: 【Objective】The effects of nitrogen form and ratio on carbon-nitrogen metabolism and related enzyme activities in the leaves of Ginkgo biloba seedlings were investigated to provide a theoretical basis for N fertilizer management of Ginkgo biloba. 【Method】Using 1-year-old seedlings of Ginkgo biloba as test materials, a greenhouse pot experiment was performed with 5 ratios of m(NH₄⁺-N):m(NO₃-N), namely T₁(100:0), T₂(75:25), T₃(50:50), T₄(25:75), and T₅(0:100). We analyzed the content of total nitrogen, soluble protein, free amino acid, total carbon, soluble sugar, starch and sucrose, and the activity of amylase(AMS), sucrose synthetase(SS), sucrose phosphate synthase(SPS), nitrate reductase(NR), glutamine synthetase(GS), and glutamine synthetase(GOGAT)in the Ginkgo biloba seedlings under different treatments. 【Result】The carbon-nitrogen metabolites(except soluble protein content)and related enzyme activities in the leaves of Ginkgo biloba increased initially and then decreased, with an increase in the NO₃^--N ratio. In terms of carbon-nitrogen metabolism, the content of sucrose and free amino acids were the highest in the T₃ group, and the content of total nitrogen, total carbon, soluble sugar, soluble protein, starch, and sucrose were the highest in the T₄ group. The content of carbon-nitrogen metabolism indicators, except for soluble protein content, were the lowest in the T₁ group. In terms of enzyme activity, SS and SPS activities were the highest in the T₃ group, and AMS, NR, GS and GOGAT activities were the highest in the T₄ group. AMS, SS, and NR activities were the lowest in the T₁ group, and SPS, GS and GOGAT activities were the lowest in the T₅ group. Overall, the contents of carbon-nitrogen metabolites and related enzyme activities in T₃ and T₄ groups were significantly higher than that in other groups, but no significant differences were observed between the T₃ and T₄ groups. 【Conclusion】The effects of NH₄⁺-N and NO₃^--N mixed supply on the carbon-nitrogen metabolism of Ginkgo biloba were significantly higher than those of the single supply. The effect of 25:75 m(NH₄⁺-N):m(NO₃^--N)was the best, and appropriate adjustments should be performed on the basis of the conditions.

CLC Number:

S792.95

HUANG Dongjing, CAO Fuliang, WANG Guibin. Nitrogen form and ratio affect the carbon-nitrogen metabolism of Ginkgo biloba [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(02): 197-202.

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Nitrogen form and ratio affect the carbon-nitrogen metabolism of Ginkgo biloba

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