Genetic variation and improved parents selection of open pollination families of Picea crassifolia Kom. basing one BLUP method

doi:10.3969/j.issn.1000-2006.201812002

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (6): 53-59.doi: 10.3969/j.issn.1000-2006.201812002

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Genetic variation and improved parents selection of open pollination families of Picea crassifolia Kom. basing one BLUP method

OUYANG Fangqun¹(), QI Shengxiu², FAN Guoxia², CAI Qishan², CHEN Haiqing², GAO Wanli², HU Changshou², WANG Junhui¹^,^*()

1. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy Forestry,Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration, CAF,Beijing 100091,China
2. Dongxia Forestry Centre of Qinghai Province,Datong 810100, China

Received:2018-12-04 Revised:2019-09-02 Online:2019-11-30 Published:2019-11-30
Contact: WANG Junhui E-mail:fangqun163@163.com;wangjh808@sina.com

Abstract

Abstract:

【Objective】 The best linear unbiased prediction (BLUP) method forecasts a breeding value using a mixed linear model equation, which reflects true genetic effects with high accuracy. 【Method】 This study used two open pollination progeny tests of Picea crassifolia Kom. at 13 and 14 years old obtained from a primary clonal seed orchardto evaluate genetic variation in height. A breeding value was predicted using the BLUP method. 【Result】Our results showed that family and interaction effects between family and blocks significantly affected height. The family hereditability for height of the trees from the two open pollination progeny were 0.17 and 0.36, respectively, while the individual hereditability were both 0.07. The Pearson correlation between the original value and the breeding value of the height at family level, or at individual level, were both high, and the Pearson correlation coefficients were 0.99 and 0.86, respectively. According to the breeding value of the parents of the families, 20 elite families were chosen and the reality genetic gain reached 36% and 26%, respectively. According to the individual breeding value estimated using the BLUP method, 20 plus trees were chosen, and the expected genetic gains were 7.6% and 7.9%, respectively. 【Conclusion】By comparing the genetic gain of backward selection and forward selection, it was determined that backward selection is the better choice in high-generation breeding ofPicea crassifolia.

Key words: Picea crassifolia Kom., improved generation of parents, best linenr unbiased prediction (BLUP), combining selection, breeding value

CLC Number:

S718.46

OUYANG Fangqun, QI Shengxiu, FAN Guoxia, CAI Qishan, CHEN Haiqing, GAO Wanli, HU Changshou, WANG Junhui. Genetic variation and improved parents selection of open pollination families of Picea crassifolia Kom. basing one BLUP method[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(6): 53-59.

Figures/Tables 5

Table 1

Table 2

Mean value, variance component and genetic parameters of height of Picea crassifolia "

性状 traits	树高均值 ±SD /cm height±SD	方差分量/% percentage			遗传力 heritability		遗传增益/% genetic gain
性状 traits	树高均值 ±SD /cm height±SD	家系 $σ f 2$ family	家系与区组 $σ f × b 2$ interaction between family and block	误差 $σ e 2$ residual	家系 broad- sense H²	单株 $h i 2$ narrow individual	优良家系现实G realize of elite familie	优良单株预期ΔG expected of plus trees
试验林Ⅰ13 a树高 tree height of 13 a in testⅠ	91.1±25.9	12.36** (1.87)	142.81** (21.63)	505.06 (76.50)	0.175	0.070	36.92	7.6
试验林Ⅱ14 a树高 tree height of 14 a in testⅡ	100.1±31.6	18.41** (1.85)	195.38** (19.69)	778.73 (78.46)	0.360	0.070	26.99	7.9

Table 2

Fig.1

Table 3

Fig.2

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变异来源 sources	试验林Ⅰ13 a树高 tree of 13 a in testⅠ				试验林Ⅱ14 a树高 tree of 13 a in testⅡ
变异来源 sources	自由度	均方	F	P_r > F	自由度	均方	F	P_r > F
区组block	3	243 672		<0.000 1	9	8 769		<0.000 1
家系family	51	41 206	1.21	<0.000 1	60	3 515	1.57	<0.000 1
区组×家系block×family	149	34 007		<0.000 1	497	2 234		<0.000 1
误差error	763	10 140			3 493	766

子代测定林号 progeny tests code	区组 block	家系号 family No.	重复号 repetion No.	树高/ cm height	预测树高/ cm predicted height	育种值 breeding value	子代测定林号 progeny tests code	区组 block	家系号 family No.	重复号 repetion No.	树高/ cm height	预测树高/ cm predicted height	育种值 breeding value
Ⅰ	2	81155	1	193	120	29.42	Ⅱ	3	28	2	222	125	25.02
	3	81155	5	185	117	26.57		6	53	6	208	122	22.83
	2	81167	4	169	115	25.16		2	53	7	198	121	21.01
	3	81143	5	172	114	24.10		3	28	8	183	120	20.18
	1	81165	2	186	113	22.53		6	87	4	206	119	19.82
	2	81137	3	157	112	21.32		3	53	2	188	119	19.55
	1	81020	1	160	110	20.10		3	41	3	188	119	19.40
	4	81164	2	141	110	19.43		1	41	1	179	118	18.56
	4	81143	5	135	109	18.99		8	53	4	169	118	18.47
	3	81167	3	144	109	18.69		6	28	6	160	118	18.12
	2	81137	2	144	109	18.55		1	41	2	175	118	18.06
	4	81164	1	135	109	18.51		9	53	4	163	118	17.96
	4	83004	5	141	108	17.49		2	28	2	161	117	17.66
	3	81164	4	142	108	17.27		7	28	2	156	117	17.65
	3	83012	1	154	107	17.18		6	41	6	167	117	17.59
	3	83012	3	154	107	17.18		2	41	7	170	117	17.38
	1	81143	2	137	107	17.16		5	21	4	170	117	17.24
	2	81143	5	133	107	16.94		1	21	6	173	117	17.15
	3	81170	4	153	107	16.72		2	58	2	188	117	17.14
	2	81164	3	134	107	16.71		7	40	7	203	117	17.12

Genetic variation and improved parents selection of open pollination families of Picea crassifolia Kom. basing one BLUP method

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