The alleviating effects of selenium on cadmium-induced toxicity in tea leaves

doi:10.3969/j.issn.1000-2006.201902006

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (1): 200-204.doi: 10.3969/j.issn.1000-2006.201902006

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The alleviating effects of selenium on cadmium-induced toxicity in tea leaves

ZHANG Qing¹^,³(), WEI Shuhe²^,^*(), DAI Huiping¹^,^*(), JIA Genliang³

1. Shaanxi Provincial Key Laboratory of Bio-resources, College of Biological Science & Engineering, Shaanxi University of Technology, Hanzhong 723001, China
2. Key Laboratory of Pollution Ecology and Environment Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
3. College of Science, Northwest A&F University, Yangling 712100, China

Received:2019-02-13 Revised:2019-08-08 Online:2020-02-08 Published:2020-02-02
Contact: WEI Shuhe,DAI Huiping E-mail:578809967@qq.com;shuhewei@iae.ac.cn;daihp72@aliyun.com

Abstract

Abstract:

【Objective】 We examined the effects of selenium (Se) on the growth and physiological characteristics of tea plants subjected to cadmium (Cd) based on a culture solution experiment, the findings of which will provide a theoretical basis for ensuring the safety of tea drinking and the comprehensive development and utilization of tea. 【Method】 As experimental material, we used ‘Longjing 43’tea trees leaves planted in the Hanzhong area of Shaanxi Province, China, which were exposed to the following seven combinations of Cd and Se in 1/2 Hoagland solution: 10 mg/L Cd, 0.5 mg/L Se, 1.5 mg/L Se, 3.0 mg/L Se, 0.5 mg/L Se + 10.0 mg/L Cd, 1.5 mg/L Se + 10.0 mg/L Cd, and 3.0 mg/L Se + 10.0 mg/L Cd. Following treatment, we determined the physiological responses associated with the alleviation of Cd toxicity in tea leaves treated with different concentrations of Se (0.5-3.0 mg/L), and measured the biomass of tea leaves, and the concentrations of Se and Cd in these leaves. 【Result】 Compared with the control treatment without either Cd or Se addition, the treatment with Cd alone promoted a significant decrease in the concentration of photosynthetic pigments, antioxidant enzymes [catalase (CAT), ascorbate peroxidase(APX) and glutathione peroxidase (GPX)], biomass, and the concentrations of soluble sugars and soluble protein in tea leaves, whereas the contents of malondialdehyde (MDA), hydrogen peroxide (H₂O₂), and proline were significantly increased. In samples treated with Se alone, the above indices (e.g. biomass, and the concentrations of soluble sugars, soluble protein, and photosynthetic pigments, antioxidant enzymes activities in tea leaves) were significantly improved to differing extents compared with the Cd only treatment, particularly in response to treatment with 1.5 mg/L. In the combined Cd and Se treatments, we noted that the indices were improved to varying degrees in response to increases in Se concentration compared with the Cd only treatment, with the most pronounced improvement being obtained in response to the combined treatment of 1.5 mg/L Se + 10.0 mg/L Cd. The application of selenium reduced the accumulation of cadmium in tea leaves.【Conclusion】 To differing extents, the addition of an appropriate amount of Se can increase the activities of CAT, APX and GPX induced by Cd in tea leaves, reduce the accumulation of MDA and H₂O₂, and alleviate Cd toxicity.

Key words: tea, cadmium stress, selenium, tea leaf quality, physiological response, biomass, Hanzhong area of Shannxi Province

CLC Number:

Q945.78
S718

ZHANG Qing, WEI Shuhe, DAI Huiping, JIA Genliang. The alleviating effects of selenium on cadmium-induced toxicity in tea leaves[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(1): 200-204.

Figures/Tables 5

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References 21

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处理 treatment	叶绿素a含量 chlorophyll a content	叶绿素b含量 chlorophyll b content	类胡萝卜素含量 carotenoid content
CK	1.60±0.05 c	2.06±0.12 c	1.37±0.07 c
T1	0.96±0.02 e	1.01±0.07 e	0.72±0.03 e
T2	1.76±0.13 b	2.23±0.16 b	1.51±0.06 c
T3	1.91±0.21 a	2.73±0.19 a	1.92±0.12 a
T4	1.81±0.11 a	2.43±0.15 b	1.72±0.10 b
T5	1.16±0.07 d	1.73±0.11 d	0.93±0.05 d
T6	1.46±0.09 c	1.99±0.07 c	1.23±0.08 c
T7	1.26±0.04 d	1.83±0.10 c	1.03±0.04 d

处理 treatment	过氧化氢酶活性 CAT activity	抗坏血栓过氧化物酶活性 APX activity	谷胱曲肽过氧化物酶活性 GPX activity
CK	30.6±0.67 b	11.4±0.89 c	26.1±1.23 b
T1	10.2±0.51 e	4.62±0.15 e	9.36±0.63 f
T2	32.2±1.20 b	13.8±0.19 b	27.4±1.33 b
T3	36.4±1.62 a	18.7±0.13 a	30.8±0.66 b
T4	35.9±2.17 a	15.4±0.12 b	28.3±1.18 a
T5	13.5±1.16 d	7.72±0.55 d	12.4±0.82 e
T6	21.0±1.25 c	9.99±0.61 c	17.3±0.43 c
T7	16.3±1.01 d	8.83±0.72 c	14.7±0.56 d

处理 treatment	丙二醛含量 MDA content	过氧化氢含量 H₂O₂ content
CK	0.42±0.01 d	0.66±0.02 d
T1	1.02±0.06 a	1.26±0.08 a
T2	0.32±0.01 e	0.46±0.01 e
T3	0.26±0.01 f	0.36±0.01 f
T4	0.35±0.01 e	0.53±0.03 e
T5	0.91±0.05 b	1.01±0.07 b
T6	0.53±0.04 d	0.72±0.05 d
T7	0.76±0.04 c	0.88±0.05 c

处理 treatment	脯氨酸含量 proline content	可溶性糖含量 soluble sugar content	可溶性蛋白质含量 soluble protein content
CK	0.63±0.01 cd	0.81±0.03 d	0.71±0.05 c
T1	1.53±0.06 a	0.65±0.02 e	0.53±0.01 d
T2	0.55±0.02 d	0.82±0.04 d	0.73±0.02 c
T3	0.50±0.01 d	0.93±0.06 c	0.83±0.02 b
T4	0.40±0.01 d	1.06±0.09 b	0.90±0.04 a
T5	1.03±0.05 b	0.95±0.05 c	0.72±0.02 c
T6	0.78±0.03 c	1.25±0.08 a	0.93±0.05 a
T7	0.99±0.03 b	0.88±0.04 cd	0.76±0.03 c

处理 treatment	Cd含量/ (mg·kg^-1) Cd content	Se含量/ (mg·kg^-1) Se content	叶片生物量/ (g·株^-1) leaf biomasse
CK	0.56±0.20 e	0.46±0.19 g	10.3±0.13 b
T1	6.43±0.09 a	0.09±0.12 g	7.80±0.05 d
T2	0.41±0.59 e	3.33±0.07 c	10.61±0.08 b
T3	0.21±0.58 e	4.43±0.67 b	11.98±0.16 a
T4	0.11±0.53 e	5.73±0.07 a	10.4±0.17 b
T5	3.51±0.67 b	1.43±0.16 f	8.6±0.04 d
T6	0.99±0.46 d	1.83±0.09 e	10.7±0.09 b
T7	2.26±0.70 c	2.39±0.26 d	9.7±0.03 c

The alleviating effects of selenium on cadmium-induced toxicity in tea leaves

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