Key bioactive substances and their antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves collected from natural populations

doi:10.3969/j.issn.1000-2006.201901045

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (2): 10-16.doi: 10.3969/j.issn.1000-2006.201901045

Special Issue: 青钱柳叶有效成分专题

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Key bioactive substances and their antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves collected from natural populations

LIN Yuan(), CHEN Pei, ZHOU Mingming, SHANG Xulan, FANG Shengzuo^*()

Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Received:2019-01-30 Revised:2019-04-21 Online:2020-03-30 Published:2020-04-01
Contact: FANG Shengzuo E-mail:381209292@qq.com;fangsz@njfu.edu.cn

Abstract

Abstract:

【Objective】 Cyclocarya paliurus (Batal.) Iljinskaja is a multiple-use tree species with applications in timber, ornamental and medicinal industries. However, recently, the most common use ofC. paliurus (Batal.) Iljinskaja in the market is the preparation of nutraceutical tea with health benefits using its leaves. The objective of the present study was to select superior provenances or natural populations of C. paliurus (Batal.) Iljinskaja for medicinal purposes based on their content of key bioactive substances and their antioxidant activity. 【Method】 This study measured, using water extraction, the total flavonoid, total triterpenoid, and total polyphenol content and antioxidant activity in C. paliurus (Batal.) Iljinskaja leaves collected from 33 natural populations and analyzed the correlation between content and antioxidant activity. 【Result】The flavonoid, triterpenoid and polyphenol content exhibited significant variation in the extracts of C. paliurus (Batal.) Iljinskaja leaves collected from different natural populations. The total flavonoid content in the leaves was 6.79-20.91 mg/g, whereas the total triterpenoid and total polyphenol content was 15.67-44.63 and 10.53-33.04 mg/g, respectively. Hierarchical cluster analysis indicated that the 33 natural populations were classified into three, four and three distinct groups based on the total flavonoid, total triterpenoid and total polyphenol content in their leaves, respectively. Correlation analysis showed a significant positive correlation between total flavonoid, total triterpenoid and total polyphenol content; these key bioactive substances were also significantly correlated with DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical-scavenging activity, ABTS [2, 2'-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid)] free radical-scavenging activity, and total reducing power. 【Conclusion】Cyclocarya paliurus (Batal.) Iljinskaja natural populations from Anhui Jixi (S2), Guizhou Shijie (S15), Hubei Hefeng (S19) and Hubei Wufeng (S20) are excellent populations for preparing nutraceutical tea with a high content of key bioactive substances and strong antioxidant activity.

Key words: Cyclocarya paliurus (Batal.) Iljinskaja, flavonoid, triterpenoid, polyphenol, antioxidant activity, natural populations

CLC Number:

S718

LIN Yuan, CHEN Pei, ZHOU Mingming, SHANG Xulan, FANG Shengzuo. Key bioactive substances and their antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves collected from natural populations[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(2): 10-16.

Figures/Tables 4

Table 1

Coordinates and the key climatic factors of different Cyclocarya paliurus (Batal.) Iljinskaja natural populations "

居群 population	经度(E) longitude	纬度(N) latitude	年均气温/℃ average annual temperature	年均降雨量/mm average annual rainfall	海拔/m altitude	年日照时长/h annual sunshine hours	居群 population	经度(E) longitude	纬度(N) latitude	年均气温/℃ annual average temperature	年均降雨量/mm average annual rainfall	海拔/m altitude	年日照时长/h annual sunshine hours
S1	117°31'48″	30°01'12″	13.88	1 775	399.60	1 800	S18	115°33'00″	31°43'12″	12.20	1 368	928.75	1 763
S2	118°27'00″	30°13'48″	13.83	1 657	617.00	1 926	S19	110°25'12″	29°52'48″	11.27	1 499	1 130.90	1 342
S3	118°53'24″	30°09'00″	12.13	1 726	738.18	1 784	S20	110°54'00″	30°11'24″	12.60	1 377	969.17	1 533
S4	116°32'24″	31°01'12″	12.31	1 606	770.45	1 969	S21	112°01'48″	24°55'12″	17.40	1 502	854.18	1 758
S5	117°00'36″	26°35'24″	17.36	1 821	556.81	1 900	S22	110°19'48″	28°52'48″	14.76	1 492	670.27	1 266
S6	117°55'48″	25°25'48″	18.06	1 593	477.00	1 950	S23	114°31'48″	27°37'48″	15.29	1 691	558.14	1 737
S7	118°45'36″	27°55'48″	15.80	1 998	924.70	1 738	S24	114°06'00″	26°30'36″	13.23	1 816	554.40	1 511
S8	112°04'51″	24°31'49″	18.90	1 753	1 250.00	1 382	S25	114°53'24″	28°55'12″	13.48	1 655	825.20	1 600
S9	106°20'24″	24°27'36″	15.61	1 364	1 478.00	1 906	S26	105°52'12″	33°21'36″	12.96	714	1 218.22	1 526
S10	104°57'00″	24°36'36″	16.66	1 250	1 762.18	1 475	S27	103°46'48″	28°58'12″	15.86	1 168	1 190.50	965
S11	109°53'24″	25°37'12″	14.53	1 629	1 307.00	1 544	S28	104°51'36″	32°25'12″	14.08	795	1 607.83	900
S12	110°22'48″	25°55'12″	15.49	1 580	842.00	1 308	S29	119°38'24″	30°24'36″	13.43	1 472	576.71	1 849
S13	108°22'48″	26°22'12″	15.10	1 265	1 156.00	1 236	S30	121°13'12″	29°45'36″	14.05	1 527	724.25	1 850
S14	109°14'24″	26°20'24″	16.14	1 311	727.00	1 320	S31	119°11'24″	27°54'36″	11.78	2 119	1 176.57	1 846
S15	108°06'36″	27°21'00″	13.30	1 256	1 239.00	966	S32	121°48'24″	29°48'00″	15.72	1 425	167.50	1 861
S16	108°30'36″	27°44'24″	14.48	1 231	985.43	1 100	S33	119°47'23″	27°52'48″	13.89	1 913	927.40	1 698
S17	112°00'00″	33°28'48″	11.54	834	875.67	900

Table 1

Fig.1

Table 2

Table 3

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居群 population	IC₅₀/(mg·mL^-1)		总还原力 (吸光度值) total reducing power	居群 population	IC₅₀/(mg·mL^-1)		总还原力 (吸光度值) total reducing power
居群 population	DPPH	ABTS	总还原力 (吸光度值) total reducing power	居群 population	DPPH	ABTS	总还原力 (吸光度值) total reducing power
S1	2.16±0.04 k	3.94±0.09 m	0.40±0.00 l	S18	3.64±0.05 q	5.54±0.06 q	0.31±0.00 qr
S2	1.26±0.04 c	1.70±0.05 b	0.51±0.01 ef	S19	0.96±0.02 b	1.78±0.06 bc	0.56±0.01 c
S3	1.71±0.03 i	3.26±0.06 k	0.42±0.01 l	S20	0.90±0.02 ab	1.29±0.06 a	0.47±0.00 h
S4	1.75±0.04 i	3.27±0.06 k	0.51±0.00 e	S21	2.78±0.02 o	4.32±0.05 n	0.35±0.00 n
S5	2.78±0.05 o	3.63±0.08 l	0.54±0.01 d	S22	2.45±0.05 m	2.27±0.07 e	0.45±0.00 j
S6	1.47±0.05 ef	2.60±0.07 gh	0.51±0.00 f	S23	2.47±0.03 mn	3.62±0.09 l	0.34±0.00 o
S7	1.54±0.02 g	2.64±0.05 h	0.46±0.00 i	S24	3.63±0.02 q	6.28±0.08 s	0.30±0.00 rs
S8	1.64±0.02 h	3.04±0.05 j	0.50±0.01 g	S25	3.35±0.05 p	8.75±0.10 t	0.32±0.00 o
S9	1.42±0.03 e	1.82±0.05 c	0.55±0.00 d	S26	1.47±0.02 ef	2.05±0.05 d	0.65±0.01 a
S10	4.44±0.05 s	4.73±0.10 o	0.32±0.01 p	S27	1.35±0.02 d	1.69±0.06 b	0.56±0.00 bc
S11	1.47±0.03 ef	2.49±0.05 fg	0.54±0.00 d	S28	3.93±0.02 r	3.97±0.09 m	0.31±0.01 qr
S12	2.03±0.05 j	3.58±0.13 l	0.44±0.00 k	S29	1.55±0.03 g	2.86±0.05 i	0.42±0.01 k
S13	1.53±0.04 fg	2.59±0.05 gh	0.56±0.00 c	S30	2.25±0.03 l	1.24±0.05 a	0.37±0.00 m
S14	3.64±0.06 q	5.01±0.10 p	0.31±0.00 pq	S31	2.75±0.05 o	5.67±0.10 r	0.37±0.00 n
S15	0.88±0.02 a	1.20±0.05 a	0.54±0.01 d	S32	3.79±0.07 q	6.25±0.08 s	0.28±0.00 s
S16	1.33±0.03 d	2.43±0.05 f	0.57±0.01 b	S33	1.99±0.04 j	3.63±0.07 l	0.44±0.01 jk
S17	2.52±0.03 n	3.99±0.09 m	0.40±0.00 m

指标 index	总三萜含量 total triterpenoids content	总多酚含量 total polyphenols content	IC₅₀ (DPPH)	IC₅₀ (ABTS)	总还原力 total reducing power
总黄酮含量 total flavonoids content	0.589^**	0.814^**	-0.775^**	-0.557^**	0.610^**
总三萜含量 total tierpenoids content		0.694^**	-0.791^**	-0.578^**	0.596^**
总多酚含量 total polyphenols content			-0.877^**	-0.755^**	0.775^**
IC₅₀ (DPPH)				0.793^**	-0.835^**
IC₅₀(ABTS)					-0.734^**

Key bioactive substances and their antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves collected from natural populations

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