Abstract: Axillary buds were induced when the explant sources were the aseptic seedlings without roots of P.elliottii.6-benzylaminopurine(6-BA) and kinetin(KT) were both effective at inducing buds formation,but 6-BA was more potent than KT.6-BA in combination with NAA in modified Gresshoff and Doy(GD) medium increased bud production compared with the medium with cytokinin only.The concentration of 6-BA and NAA and the length of exposure to the cytokinin significantly influenced bud induction.The highest inducing rate of axillary buds was up to 95% on the modified GD medium supplemented with 5.0 mg/L6-BA and 0.1 mg/L NAA for 4～5 weeks.The buds were propagated most rapidly when subcultured on the medium of modified GD containing 3.5 mg/L 6-BA and 0.05 mg/L NAA.The addition of NAA(0.05～0.1 mg/L) or activated charcoal(0.5 g/L) to basal medium could greatly promote slash pine shoots elongation and growth.Reduced concentration of minerals failed to enhance shoot elongation.Roots were formed when excised shoots were planted on modified GD 1/2 medium supplemented with 0.05 mg/L NAA for 4 weeks.The rooting rate was 53.3%.The surviving rate was 85% when the rooting shoots were moved to the greenhouse.