Abstract: In order to understand the the of fine-root in forest ecosystems, fine-root biomass and related nutrients in a subtropical forest were sludied in Xiaokeng forested watershed of Southern Anhui. The results showed that the fine-root biomass was greater in the surface soil layer thau that in the low ones in all sampling stands. The contents of N, P and K in fine-root gradoally decreased from surface to low soil layer, and so were the storages of N, P and K in fine-root too. Along a successional gradient nf the secondary evergreen broad-leaved forest, the fine-root biomass increased with an inerease nf staud age. There existed significant difference in fine-root biomass between different aged stands. The content of P in flue-root was higher iu the young stand than that in the old stand. The contents of N, P and K in fine-root were signifieamly different between differcnt aged stands of subtropical evergreen broad-leaved tbrest.