Abstract: The differences of landscape of bird roost trees has a positive role in creating habitat conditions and attracting birds artificially. One hundred eighty nine roost trees for 12 kinds of birds were found in Kunming city, Yunan province. Principal component analysis with 28 landscape factors of roost trees was conducted. The results of two principle component analyses showed that Pycnonotus xanthorrhous preferred to roost in trees with tall buildings in the West. Carduelis ambigua and Passer montanus preferred to roost in trees with shorter sunshine hours and good canopy density. Roost trees for Carduelis ambigua are always noiseful while roost trees for Passer montanus are noiseless. As common birds in cities, they show high degree on adaptive ability to city noise and light in choosing roost trees. The other 9 bird species prefer roost trees without tall buildings in the West, poor canopy density but long light hours. The longer light hours are conducive to maintaining relatively constant temperature. Warming at night may be the key factor for birds to choose roost trees in Kunming.