JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2012, Vol. 36 ›› Issue (04): 84-88.doi: 10.3969/j.jssn.1000-2006.2012.04.017

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Codon optimization of cellobiohydrolase gene of Aspregillus niger and its expression in Pichia pastoris

LI Guoqing, FAN Song, PEI Jianjun, ZHAO Linguo*, GAO Shufeng   

  1. College of Chemical Engineering, Nanjing Forestry University, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing 210037, China
  • Online:2012-07-07 Published:2012-07-07

Abstract: NCBI analysis revealed that there were sixteen active sites in cellobiohydrolase gene(cbh1) from Aspregillus niger, and were found rare codons around six active sites. To improve expression efficiency of recombinant cellobiohydrolase in Pichia pastoris KM71H, those rare codons around active site of cbh1 were replaced with preferable codons used in P. pastoris by long-distance inverse PCR. The recombinant plasmid pPICZαA-cbh1m which contained the modified cbh1 sequence was transformed in P. pastoris KM71H. After methanol induction, the expression level of cbh1m was 17% higher than that of cbh1.

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