Abstract: Several factors influencing αglucosidase production with the recombinant Escherichia coli haroring the JM109/pBV220GLZ were investigated. The results showed that the best inducement conditions were as follows: induction temperature 40 ℃, induction time 14 h, and at OD600 of 2.0. To determine the optimum conditions of immobilization, the concentration of sodium alginate， CaCl2， and glutaraldehyde, and solidifying time were studied. The results showed that the optimum operations were: 20 g／L sodium alginate， 20 g／L CaCl2， 1.0 ％ glutaraldehyde， solidifying time 2 h. Under the optimum condition， the recovery rate reached 81 ％. After 15 times repeated operations， the enzyme activity remained 75 ％， showing good operational stability. Thus， the superior stability of the recombinant enzyme laid the base for largescale application.