Cloning and function analysis of PnsGA20ox1 gene in Populus simonii ×P. nigra

doi:10.3969/j.issn.1000-2006.2013.06.003

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2013, Vol. 37 ›› Issue (06): 11-16.doi: 10.3969/j.issn.1000-2006.2013.06.003

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Cloning and function analysis of PnsGA20ox1 gene in Populus simonii ×P. nigra

ZHANG Lijie,ZHANG Kaixuan,WEI Zhigang^*

State Key Laboratory of Tree Genetics and Breeding, School of Forestry of Northeast Forestry University,Harbin 150040,China

Online:2013-12-18 Published:2013-12-18

Abstract

Abstract: The gene fragment of GA20ox was cloned from Populus simonii&#215;P. nigra by using degenerate primers based on the conservative of GA20ox gene homologous genes. The full-length of cDNA was isolated by using RACE.The cDNA was 1 678 bp and encoded a protein of 385 amino acids with Mr= 44. 04 ku and pI=5.97.The nucleotides shared 99% identity with the GA20ox1 gene of Populus trichocarpa.It was named PnsGA20ox1. Quantitative real-time PCR analysis showed the PnsGA20ox1 gene expressed in all organs of Populus simonii&#215;P.nigra, and the expression level of PnsGA20ox1 was the highest in mature xylem but the least in internode organizations.The transgenic tobacco showed that the holocellulose content and fiber length in overexpression of the PnsGA20ox1 gene of tobacco improved comparised with the control,while there was no significant difference in lignin content comparised with the control.

CLC Number:

Q786

ZHANG Lijie,ZHANG Kaixuan,WEI Zhigang. Cloning and function analysis of PnsGA20ox1 gene in Populus simonii ×P. nigra[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2013, 37(06): 11-16.

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Cloning and function analysis of PnsGA20ox1 gene in Populus simonii ×P. nigra

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