Laboratory decomposition research of Lophodermium on the litter needles from Pinus armandii

doi:10.3969/j.issn.1000-2006.2014.03.015

Laboratory decomposition research of Lophodermium on the litter needles from Pinus armandii

YANG Chunlin,XU Xiulan,TIAN Sha,WEN Zilan,LIU Yinggao,JIANG Xinhua,ZHANG Yan,LIU Huitao

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (03) : 77-82.

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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (03) : 77-82. DOI: 10.3969/j.issn.1000-2006.2014.03.015

Laboratory decomposition research of Lophodermium on the litter needles from Pinus armandii

YANG Chunlin,XU Xiulan,TIAN Sha,WEN Zilan,LIU Yinggao^*,JIANG Xinhua,ZHANG Yan,LIU Huitao

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Abstract

In order to study the saprophytic ability of Lophodermium spp. and mechanism of each cellulase produced in the process of decomposing litters, newly litter needles from Pinus armandii were used as the decomposition material to conduct a decomposition experiment in pure culture. Five species of Lophodermium(L. conigenum, L. pinastri, L. kumaunicum, L. sichuanense, L. erlangshanense)were cultivated, in which the needles were used as the sole carbon source. The weight loss rate of the needles and the cellulase enzyme activities(Filter paper activity(FPA), Endoglucanase(EG), Cellobiohydrolase(CBH), β-glucosidase(BGL))were determined in the test. The correlation analysis showed that there was a certain synergistic effect among EG, CBH and BGL(except L. pinastri and L. erlangshanense), especially among CBH and EG. Cellulose decomposition was restricted by each enzyme activity, the litter needles could be decomposed faster with higher synergistic effect and high enzyme activity. The mass loss rates caused by five Lophodermium spp. was between 8.5%-11.8%, and which declined in the following order: L. pinastri>L. sichuanense>L. kumaunicum>L. conigenum>L. erlangshanense. The five kinds of Lophodermium spp. can produce cellulase, and they have different levels of synergistic effect among EG, CBH and BGL. They all had certain ability to decompose the needles, and they are facultative parasites, but they have weak saprophytism.

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YANG Chunlin,XU Xiulan,TIAN Sha,WEN Zilan,LIU Yinggao,JIANG Xinhua,ZHANG Yan,LIU Huitao. Laboratory decomposition research of Lophodermium on the litter needles from Pinus armandii[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2014, 38(03): 77-82 https://doi.org/10.3969/j.issn.1000-2006.2014.03.015

References

[1] 林英任. 中国真菌志·第四十卷·斑痣盘菌目[M]. 北京: 科学出版社, 2012.
[2] 宋玉双, 何秉章, 王福生. 我国松落针病研究的新进展[J]. 森林病虫通讯, 1994(2): 42-46.Song Y S, He B Z, Wang F S. New progress in study of needle cast of conifers in our country[J]. Forest Pest and Disease, 1994(2): 42-46.
[3] 林英任. 松落针病的主要病原—松针散斑壳的研究[J]. 安徽农学院学报, 1987(4): 21-28.Lin Y R. Study on the needle cast fungus of pine, Lophodermium pinastri[J]. Journal of Anhui Agricultural College, 1987(4): 21-28.
[4] Asai E I, Hata K, Futai K. Effect of simulated acid rain on the occurrence of Lophodermium on Japanese black pine needles[J]. Mycological Research, 1998, 102(11): 1316-1318.
[5] 高国平, 王丽丽, 祁金玉, 等. 赤松针叶上4种散斑壳真菌致病性接种试验[J]. 辽宁林业科技, 2009(2): 13-15.Gao G P, Wang L L, Qi J Y, et al. Inoculation test on pathogenicity of 4 Lophodermium spp. on needle of Pinus densiflora[J]. Liaoning Forestry Science and Technology, 2009(2): 13-15.
[6] 黄荣雁, 王国娟, 陈钦华, 等. 油松常见2种散斑壳属真菌致病性的研究[J]. 防护林科技, 2010(4): 39-41.Huang R Y, Wang G J, Chen Q H, et al. Pathogenicity of 2 common Lophodermium spp. of Pinus tabulaeformis[J]. Protection Forest Science and Technology, 2010(4): 39-41.
[7] 骆军, 刘应高, 黄晓丽, 等. 四川松针上散斑壳菌致病毒素的确定及其致病性差异研究[J]. 林业科学研究, 2010, 23(5): 685-689.Luo J, Liu Y G, Huang X L, et al. Pathotox in determination of Lophodermium on pines in Sichuan province and their differences in pathogenicity[J]. Forest Research, 2010, 23(5): 685-689.
[8] 刘应高, 邱德勋. 云南松针上散斑壳的培养类型[J]. 菌物系统, 1997, 16(3): 224-229.Liu Y G, Qiu D X. Cultural types of Lophodermium of Pinus yunnanensis needles[J]. Mycosystema, 1997, 16(3): 224-229.
[9] Deckert R J, Melville L H, Peterson R L. Structural features of a Lophodermium endophyte during the cryptic life-cycle phase in the foliage of Pinus strobes[J]. Mycological Research, 2001, 105(8): 991-997.
[10] 高国平, 王月. 油松落针病的病原菌生物学特性及其侵染循环[J]. 东北林业大学学报, 2004, 32(6): 87-88.Gao G P, Wang Y. Biological characteristic of the pathogen of needle cast on Chinese pine and its infection cycle[J]. Journal of Northeast Forestry University, 2004, 32(6): 87-88.
[11] 潘欣, 刘应高, 邹立扣, 等. 散斑壳属Lophodermium spp. ITS区的序列分析[J]. 生物技术, 2006, 16(4): 5-7.Pan X, Liu Y G, Zou L K, et al. Cloning and sequenceing of the ITS regions of Lophodermium spp.[J]. Biotechnology, 2006, 16(4): 5-7.
[12] Stenstrom E, Ihrmark K. Identification of Lophodermium seditiosum and L. pinastri in Swedish forest nurseries using species-specific PCR primers from the ribosomal ITS region[J]. Forest Pathology, 2005, 35(3): 163-172.
[13] Deckert R J, Hsiang T, Peterson R L. Genetic relationships of endophytic Lophodermium nitens isolates from needles of Pinus strobes[J]. Mycological Research, 2002, 106(3): 305-313.
[14] 王士娟, 林英任, 侯成林, 等. 基于rDNA-ITS序列的散斑壳属系统发育学初步研究[J]. 菌物学报, 2010, 29(2): 199-205.Wang S J, Lin Y R, Hou C L, et al. A preliminary study on phylogeny of Lophodermium based on rDNA-ITS sequences[J]. Mycosystema, 2010, 29(2): 199-205.
[15] Feio S, Fonseca N, Gigante B, et al. Combating the parasite Lophodermium seditiosum in nurseries using the compound methyl cis-7-oxo-deisopropyl-dehydroabietate[J]. Silva Lusitana, 1995, 3(1): 23-36.
[16] 黄华艳, 吴耀军, 蓝肖. 银杉落针病的初步研究[J]. 中国森林病虫, 2008, 27(6): 1-3.Huang H Y, Wu Y J, Lan X. Lophodermium sp. on Cathaya argyrophylla[J]. Forest Pest and Disease, 2008, 27(6): 1-3.
[17] 师光开, 罗建堂, 侯成林. 华山松上的斑痣盘菌[J]. 菌物学报, 2010, 29(2): 159-163.Shi G K, Luo J T, Hou C L. Species of the Rhytismataceae on Pinus armandii[J]. Mycosystema, 2010, 29(2): 159-163.
[18] Koide K, Osono T, Takeda H. Colonization and lignin decomposition of Camellia japonica leaf litter by endophytic fungi[J]. Mycoscience, 2005, 46(5): 280-286.
[19] Osono T, Hirose D. Colonization and lignin decomposition of pine needle litter by Lophodermium pinastri[J]. Forest Pathology, 2011, 41(2): 156-162.
[20] 严海元, 辜夕容, 申鸿. 森林凋落物的微生物分解[J]. 生态学杂志, 2010, 29(9): 1827-1835.Yan H Y, Gu X R, Shen H. Microbial decomposition of forest litter[J]. Chinese Journal of Ecology, 2010, 29(9): 1827-1835.
[21] 刘应高, 潘欣, 庄启国, 等. 四川松树6种散斑壳菌记述[J]. 中国森林病虫, 2004, 23(4): 9-12.Liu Y G, Pan X, Zhuang Q G, et al. Six species of Lophodermium chev. on pines in Sichuan province[J]. Forest Pest and Disease, 2004, 23(4): 9-12.
[22] 刘应高, 潘欣, 杨静, 等. 华山松上散斑壳属一新种[J]. 菌物学报, 2009, 28(4): 473-475.Liu Y G, Pan X, Yang J, et al. A new species of the genus Lophodermium on Pinus armandii[J]. Mycosystema, 2009, 28(4): 473-475.
[23] Rowland A P, Roberts J D. Lignin and cellulose fractionation in decomposition studies using acid-detergent fiber methods[J]. Communications in Soil Sciences and Plant Analysis, 1994, 25(3-4):269-277.
[24] 中国林业科学研究院林业研究所.LY/T 1271—1999.森林植物与森林枯枝落叶层全氮、磷、钾、纳、钙、镁的测定[S].北京:中国标准出版社,1999.
[25] 中国林业科学研究院林业研究所.LY/T 1269—1999.森林植物与森林枯枝落叶层全氮的测定[S].北京:中国标准出版社,1999.
[26] 中国林业科学研究院林业研究所.LY/T 1270—1999.森林植物与森林枯枝落叶层全硅、铝、钙、镁、钾、钠、磷、碳、锰、铜、锌的测定[S].北京:中国标准出版社,1999.
[27] 许玉林, 郑月霞, 叶冰莹, 等. 一株纤维素降解真菌的筛选及鉴定[J]. 微生物学通报, 2013, 40(2): 220-227.Xu Y L, Zheng Y X, Ye B Y, et al. Isolation and identification of a cellulose degrading fungi[J]. Microbiology, 2013, 40(2): 220-227.
[28] 全国食品发酵标准化中心.QB2583—2003. 纤维素酶制剂[S]. 北京: 中国标准出版社, 2003.
[29] Reddy N, Yang Y. Biofibers from agricultural by products for industrial applications[J]. Trends Biotechnol, 2005, 23(1):22-27.
[30] Himmel M E, Ding S Y, Johnson D K, et al. Biomass recalcitrance: engineering plants and enzymes for biofuels production[J]. Science, 2007, 315: 804-807.
[31] Van Wyk, J P. Biotechnology and the utilization of biowaste as a resource forbioproduct development[J]. Trends Biotechnol, 2001, 19(5): 172-177.
[32] 韩业君, 陈洪章. 植物细胞壁蛋白与木质纤维素酶解[J]. 化学进展, 2007, 19(7-8): 1153-1158.Han Y J, Chen H Z. Plant cell wall proteins & enzymatic hydrolysis of lignocellulose[J]. Progress in Chemistry, 2007, 19(7-8): 1153-1158.
[33] Wang L S, Zhang Y Z, Gao P J, et al. Changes in the structural properties and rate of hydrolysis of cotton fibers during extended enzymatic hydrolysis[J]. Biotechnol Bioeng, 2006, 93(3): 443-456.
[34] 芦光新, 陈秀蓉, 杨成德, 等. 真菌分泌纤维素酶与2种草坪草凋落物有机质降解相互关系的研究[J]. 草地学报, 2011, 19(6): 954-959.Lu G X, Chen X R, Yang C D, et al. Relationship between cellulose by Microdochium bolleyi and total organic matter degradation of two kinds of lawn grass litter[J]. Acta Agrestia Sinica, 2011, 19(6): 954-959.
[35] Osono T. Effects of prior decomposition of beech needle litter by phyllosphere fungi on substrate utilization by fungal decomposers[J]. Mycoscience, 2003, 44(1): 41-45.
[36] Watson D, Wilson D, Walker L. Synergism in binary mixtures of Thermobifida fusca cellulases Cel6B, Cel9A, and Cel5A on BMCC and Avicel[J]. Appl Biochem Biotechnol, 2002, 101(2): 97-111.
[37] 陈荣珠, 张明亮, 蔡少丽, 等. 浅谈纤维素酶及其应用[J]. 农产品加工(学刊), 2013(4): 55-58.Chen R Z, Zhang M L, Cai S L, et al. Brief review on cellulase and the application of cellulose[J]. Academic Periodical of Farm Products Processing, 2013(4): 55-58.
[38] 张小梅, 李单单, 王禄山, 等. 纤维素酶家族及其催化结构域分子改造的新进展[J]. 生物工程学报, 2013, 29(4): 422-433.Zhang X M, Li D D, Wang L S, et al. Molecular engineering of cellulase catalytic domain based on glycoside hydrolase family[J]. Chinese Journal of Biotechnology, 2013, 29(4): 422-433.

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