Abstract: 【Objective】This study was undertaken to evaluate the growth and fruiting characters of three blueberry cultivars ‘O’Neal’, ‘Misty’ and ‘Legacy’ introduced into Nanjing. 【Method】 Plant height, crown width, maturity period, yield, fruit size, fruit weight, fruit hardness, fruit quality, and antioxidant system were investigated. 【Result】 All plants grew better under the rain-shelter cultivation condition than under the open cultivation. Among the three cultivars, ‘Legacy’ grew better than ‘O’Neal’, and ‘O’Neal’ grew better than ‘Misty’. The flowers of all three cultivars were bigger under the rain-shelter cultivation than under the open cultivation. The flower size between ‘Legacy’ and ‘Misty’ was significantly different, and the flower length between ‘O’Neal’ and ‘Misty’ was also significantly different. There was no significant difference about fruit size between three cultivars, however, fruit firmness increased under the rain-shelter cultivation. The fruit maturity period of ‘O’Neal’ was the earliest, and followed by ‘Misty’ and ‘Legacy’. The yield of ‘Legacy’ was the highest, followed by ‘Misty’ and ‘O’Neal’. ‘Legacy’ had higher soluble solids and anthocyanin content, lower acid content, with sweet flavor. ‘Misty’ had higher acid and polyphenol content with slight sour flavor. Polyphenol, soluble solids and anthocyanin content, and superoxide dismutase(SOD)activity in fruit were lower, whereas acid content and peroxydase(POD)activity in fruit were higher under rain-shelter cultivation than that under the open cultivation. ‘Misty’ had lower generation rate of O^<sup>-_·2 and malondialdehyde(MDA)content under both the rain-shelter and open cultivations indicating strong antioxidant capacity.【Conclusion】The three blueberry cultivars all grew well in Nanjing. The fruit maturity period of ‘O’Neal’ was the earliest, the fruit taste of ‘Legacy’ was the best, and the yield of ‘Misty’ was the highest. Under the rain-shelter cultivation, all plants grew better, the production of free radicals in fruit was decreased, and fruit firmness was improved.