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Anti-inflammatory activity of polyphenol extracts from blueberry fruit YAO Bei, ZHAO Huifang, WU Wenlong, LI Weilin JOURNAL OF NANJING FORESTRY UNIVERSITY    2019, 43 (03): 152-156.   DOI: 10.3969/j.issn.1000-2006.201805013 Abstract （1630）      PDF（pc） （1513668KB）（1241）       Knowledge map    Save 【Objective】 This study was performed to reveal the anti-inflammatory activity and mechanism of polyphenol extracts from blueberry fruit. 【Method】 The macrophage model was used to investigate the effects of polyphenols extracted from blueberry fruit on the inducible nitric oxide synthase(iNOS)gene and the cyclooxygenase-2(COX-2)gene expression and the NO secretion. 【Result】Blueberry polyphenol extracts significantly decreased NO secretion of lipopolysaccharide(LPS)-induced RAW264.7 macrophages, and higher concentrations of the polyphenol extract resulted in lower NO secretion. Blueberry polyphenol extracts lowered the expression of iNOS and COX-2 genes. The anti-inflammatory activity of polyphenol extracts from five blueberry cultivars was different in this test.【Conclusion】Blueberry polyphenol extract has anti-inflammatory activity by reducing NO secretion and inhibiting the expression of iNOS and COX-2 genes, thus alleviating the occurrence and development of the inflammation of LPS-induced RAW264.7 macrophages. Reference | Related Articles | Metrics | Comments（0）

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An observation on three highbush blueberry cultivars cultivated in Nanjing YANG Haiyan, DONG Shanshan, Lü Lianfei, HUANG Zhengjin, WU Wenlong, LI Weilin JOURNAL OF NANJING FORESTRY UNIVERSITY    2019, 43 (03): 157-162.   DOI: 10.3969/j.issn.1000-2006.201805005 Abstract （1934）      PDF（pc） （1390452KB）（1341）       Knowledge map    Save 【Objective】This study was undertaken to evaluate the growth and fruiting characters of three blueberry cultivars ‘O’Neal’, ‘Misty’ and ‘Legacy’ introduced into Nanjing. 【Method】 Plant height, crown width, maturity period, yield, fruit size, fruit weight, fruit hardness, fruit quality, and antioxidant system were investigated. 【Result】 All plants grew better under the rain-shelter cultivation condition than under the open cultivation. Among the three cultivars, ‘Legacy’ grew better than ‘O’Neal’, and ‘O’Neal’ grew better than ‘Misty’. The flowers of all three cultivars were bigger under the rain-shelter cultivation than under the open cultivation. The flower size between ‘Legacy’ and ‘Misty’ was significantly different, and the flower length between ‘O’Neal’ and ‘Misty’ was also significantly different. There was no significant difference about fruit size between three cultivars, however, fruit firmness increased under the rain-shelter cultivation. The fruit maturity period of ‘O’Neal’ was the earliest, and followed by ‘Misty’ and ‘Legacy’. The yield of ‘Legacy’ was the highest, followed by ‘Misty’ and ‘O’Neal’. ‘Legacy’ had higher soluble solids and anthocyanin content, lower acid content, with sweet flavor. ‘Misty’ had higher acid and polyphenol content with slight sour flavor. Polyphenol, soluble solids and anthocyanin content, and superoxide dismutase(SOD)activity in fruit were lower, whereas acid content and peroxydase(POD)activity in fruit were higher under rain-shelter cultivation than that under the open cultivation. ‘Misty’ had lower generation rate of O<sup>-·2 and malondialdehyde(MDA)content under both the rain-shelter and open cultivations indicating strong antioxidant capacity.【Conclusion】The three blueberry cultivars all grew well in Nanjing. The fruit maturity period of ‘O’Neal’ was the earliest, the fruit taste of ‘Legacy’ was the best, and the yield of ‘Misty’ was the highest. Under the rain-shelter cultivation, all plants grew better, the production of free radicals in fruit was decreased, and fruit firmness was improved. Reference | Related Articles | Metrics | Comments（0）

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The growth and fruiting characteristics of ‘Zhaixuan’ blueberry strains in Nanjing ZHAO Huifang, Lü Lianfei, YAO Bei, WU Wenlong, LI Weilin JOURNAL OF NANJING FORESTRY UNIVERSITY    2019, 43 (03): 163-168.   DOI: 10.3969/j.issn.1000-2006.201805012 Abstract （1722）      PDF（pc） （1478487KB）（1164）       Knowledge map    Save 【Objective】Evaluate the growth characteristics and fruit quality of ‘Zhaixuan’ blueberry strains No. 3, No. 4, No. 5, No. 7, No. 8, No. 9 at Nanjing in order to screen superior breeding materials. 【Method】 The growth and fruiting characteristics including growth amount, yield, flowering and fruiting phenophase, and fruit quality of six ‘Zhaixuan’ blueberry strains were observed, and evaluated in according to blueberry breeding goals. 【Result】All the six ‘Zhaixuan’ blueberry strains were early mature, high-yielding, with excellent adaptability in Nanjing area of China. The fruit yields of the five-year-old No. 4, No. 7, No. 3 and No. 9 strains exceeded 3.40 kg per plant, the fruit matured in late May or early June, fruit hardness was around 2.00 kg/cm2, and the polyphenol and anthocyanin content of the fruits was very high. The No. 7 strain produced the earliest maturing fruit, which had a good flavor, the highest anthocyanin and polyphenol content and fresh fruit sensory score. The No. 4 strain had relatively high growth, anthocyanin and polyphenol content, the highest fruit hardness and yield per plant, and the second-highest fresh fruit sensory score. 【Conclusion】The strains ‘Zhaixuan’ No. 7 and No. 4 were comprehensively evaluated as potential candidates for excellent blueberry cultivar. Reference | Related Articles | Metrics | Comments（0）

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Physiological response of blueberry roots to Mn2+ stress in soil DONG Shanshan, LI Ningran, YANG Haiyan, WU Wenlong, Lü Lianfei, LI Weilin JOURNAL OF NANJING FORESTRY UNIVERSITY    2019, 43 (03): 169-174.   DOI: 10.3969/j.issn.1000-2006.201805010 Abstract （1628）      PDF（pc） （1765997KB）（1311）       Knowledge map    Save 【Objective】 This test was carried out to understand the tolerance of blueberry to Mn stress in soil in order to provide basic references for blueberry cultivation.【Method】 Potted blueberry cultivars ‘Gardenblue’ and ‘Tifblue’ were irrigated with 0, 1.0, 2.5, 5.0, 10.0 mmol/L Mn2+, and six physiological indicators including the generation rate of O<sup>-·2, content of hydrogen peroxide(H2O2), malondialdehyde(MDA), soluble protein(SP), ascorbic acid(AsA)and glutathione(GSH)in root were determined, the physiological response and tolerance to Mn2+ stress were evaluated.【Result】Less than 2.5 mmol/L Mn2+ added in soil was innocuous for the growth of blueberry, over 2.5 mmol/L Mn2+ added in soil had negative effect on the growth of blueberry. With the increase of Mn2+ concentration, significant changes of O<sup>-·2 generation rate, H2O2 and MDA content, as well as SP, AsA and GSH content in roots appeared at 2.5 mmol/L and more concentrations, of which, these changes of ‘Gardenblue’ were slighter than ‘Tifblue’. It is inferred blueberry has strong tolerance to Mn stress in soil, and the tolerance is based on the alterations of antioxidants such as SP, AsA and GSH. 【Conclusion】Blueberry has a regulating mechanism for Mn stress, which lead to the strong tolerance to Mn with a threshold value of 2.5 mmol/L Mn2+ concentration in soil. Reference | Related Articles | Metrics | Comments（0）