Bioinformatics and expression characteristics analysis of BpTCP8 in Betula platyphylla Suk.

doi:10.3969/j.issn.1000-2006.201811066

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (5): 67-73.doi: 10.3969/j.issn.1000-2006.201811066

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Bioinformatics and expression characteristics analysis of BpTCP8 in Betula platyphylla Suk.

AN Linjun(), LUAN Jiayu, REN Li, LI Huiyu, XIA De’an^*()

School of Forestry, Northeast Forestry University, Harbin 150040, China

Received:2018-11-30 Revised:2019-05-14 Online:2019-10-08 Published:2019-10-08
Contact: XIA De’an E-mail:937175890@qq.com;xiadean@126.com

Abstract

Abstract:

【Objective】 To reveal the function of BpTCP8 in the growth and development ofBetula platyphylla Suk., we analyzed the sequence characteristics, expression pattern in different tissues and the BpTCP8 gene response to hormones and stress.【Method】 The sequence characteristics of BpTCP8 gene were analyzed using bioinformatic methods. Besides, the expression characteristics of the BpTCP8 gene in different tissues as well as its response to hormones and stress were analyzed by real-time fluorescent quantitative PCR.【Result】 Using bioinformatic tools, it was found that the predicted amino acid sequence for the BpTCP8 gene contained a highly conserved bHLH domain, similar to that of the TCP family of transcriptional regulators. Results from qRT-PCR analyses indicated that BpTCP8 was highly expressed during the early developmental stages and during leaf senescence; BpTCP8 was also found to be upregulated in leaves with deep-splitting, apical buds, the xylem and the phloem. Different expression patterns of BpTCP8 were exhibited under different hormone treatments (GA₃, ME-JA and ABA) and abiotic stresses (high PEG, NaCl, CdCl₂ and NaHCO₃concentrations).【Conclusion】 Therefore, the BpTCP8 gene may be involved in leaf maturation and leaf shape, and in the growth of apical buds, the xylem, and the phloem of B. platyphylla Suk.. The gene is positively regulated by GA₃ and JA. BpTCP8 expression may also affect plant resistance to drought, salt, alkali and heavy metals through the ABA signaling pathway.

Key words: Betula platyphylla Suk., BpTCP8 gene, expression characteristics, hormone response, stress response

CLC Number:

S722

AN Linjun, LUAN Jiayu, REN Li, LI Huiyu, XIA De’an. Bioinformatics and expression characteristics analysis of BpTCP8 in Betula platyphylla Suk.[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(5): 67-73.

Figures/Tables 6

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References 20

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引物 perimer	正向引物 forward perimer	反向引物 reverse perimer
BpTCP8	5'-ATGATATCAAATTCAAGAGAA-3'	5'-TTAAGAACCGGTGTTCTCCT-3'
定量PCR引物 qRT-PCR perimer	5'-GCTTGATGCCACTAAGCTTGATAT-3'	5'-CCATGGAATTGTTCAGCAAGG-3'
α-Tubulin	5'-GCACTGGCCTCCAAGGAT-3'	5'-TGGGTCGCTCAATGTCAAGG-3'

Bioinformatics and expression characteristics analysis of BpTCP8 in Betula platyphylla Suk.

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