Functional conservation of Neolamarckia cadamba NcIRX9 gene in xylan biosynthesis

doi:10.3969/j.issn.1000-2006.201906069

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2019, Vol. 43 ›› Issue (6): 129-136.doi: 10.3969/j.issn.1000-2006.201906069

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Functional conservation of Neolamarckia cadamba NcIRX9 gene in xylan biosynthesis

YIN Qi(), QIN Wenqi, LIU Tingting, KANG Lu, WU Aimin^*(), DENG Xiaomei^*()

College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China

Received:2019-06-20 Revised:2019-08-06 Online:2019-11-30 Published:2019-11-30
Contact: WU Aimin,DENG Xiaomei E-mail:yinqiyinqi216@163.com;wuaimin@scau.edu.cn;dxmei2006@scau.edu.cn

Abstract

Abstract:

【Objective】Xylan is the major component of secondary wall hemicellulose in dicot plants and plays an indispensable role in maintaining plant secondary wall integrity and plant growth. Xylan also greatly impacts wood properties and biomass energy. The study described here explores theIRX9 gene of Neolamarckia cadamba, a fast-growing tree species in southern China. Furthermore, the role of NcIRX9 in xylan biosynthesis is also explored. 【Method】 Bioinformatics was used to analyze NcIRX9 protein structure. A yellow fluorescent protein (YFP) fusion protein was constructed NcIRX9, and its subcellular localization was observed. Both NcIRX9 expression in different tissues were analyzed by qRT-PCR. In addition, by tissue sectioning and microscopic observation, chemical immunization, monosaccharide analysis, and H¹-NMR analysis, we verified the function of NcIRX9. NcIRX9 function was further validated in a mutant Arabidopsis model. 【Result】 NcIRX9 is evolutionarily conserved and contains a signal peptide at its N-terminus, closely related toArabidopsis AtIRX9, which is located in the Golgi. The NcIRX9 gene is expressed in the roots, stems, leaves, xylem and phloem, with its highest level of expression in the xylem. We determined that NcIRX9 can partially rescue the Arabidopsis mutant irx9 phenotype, as it is orthologous to AtIRX9. Moreover, this study also demonstrated that overexpression of NcIRX9 in Arabidopsis irx9 mutants increases xylose content, side chain signal enhancement, and reduces the end side chain signal enhancement. 【Conclusion】 The biological function of the NcIRX9 gene in Neolamarckia cadamba is similar to that of Arabidopsis AtIRX9 and is highly involved in xylan biosynthesis.

Key words: Neolamarckia cadamba, xylan, NcIRX9, gene function, biomass energy

CLC Number:

YIN Qi, QIN Wenqi, LIU Tingting, KANG Lu, WU Aimin, DENG Xiaomei. Functional conservation of Neolamarckia cadamba NcIRX9 gene in xylan biosynthesis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(6): 129-136.

Figures/Tables 7

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引物名称 primer name	序列(5'→3') sequence
NcIRX9-F	GGGATCCAGAGAGATGGGGT
NcIRX9-R	TCGGACTGTCATCAAGGGGA
cyclophilin-F	GACAGGAGGAGAATCTATCTATGG
cyclophilin-R	AACCTGCCCAAACACCACAT

Functional conservation of Neolamarckia cadamba NcIRX9 gene in xylan biosynthesis

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