Salt tolerance of four half-sib families of Pyrus betulaefolia Bunge from coastal areas

doi:10.3969/j.issn.1000-2006.202003044

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (5): 157-166.doi: 10.3969/j.issn.1000-2006.202003044

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Salt tolerance of four half-sib families of Pyrus betulaefolia Bunge from coastal areas

MIAO Lifei(), YU Xiaojing, ZHANG Qiuyue, FENG Chaonian^*()

College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China

Received:2020-03-14 Revised:2020-05-13 Online:2020-10-30 Published:2020-10-30
Contact: FENG Chaonian E-mail:1296928897@qq.com;fcn@njfu.edu.cn

Abstract

Abstract:

【Objective】This research investigated the differences of salt tolerance among four half-sib families of Pyrus betulaefolia Bunge in order to provide a reference for the selection and promotion of salt tolerant varieties in coastal areas.【Method】The seedlings of 4 half-sib families collected from coastal areas were used as test materials[No. YC, QD20, QD14 and LYG], which were treated with different concentrations of NaCl (0, 0.2%, 0.4%, 0.6%, 0.8%) through indoor soil culture and pot culture experiments. The growth and physiology indicators were measured to analyze the salt tolerance ability among four half-sib families of Pyrus betulaefolia Bunge such as the survival rate, biomass, root/shoot ratio, cell membrane permeability, chlorophyll content and photosynthetic characteristics. The salt tolerance of four families was comprehensively evaluated by a factor analysis and membership function method.【Result】With the increase of salt stress concentration, the survival rate of seedlings of four Pyrus betulaefolia Bunge families decreased gradually, of which YC was the highest. The root/shoot ratio increased first and then decreased. YC and QD14 reached the maximum value at 0.6% salt concentration, while QD20 and LYG reached the maximum value at 0.2% and 0.4% salt concentration respectively. Compared with the control, the seedling height, ground diameter and the total biomass of each family were significantly inhibited at 0.2% and YC decreased the least by 37.00%, 18.69% and 24.98%, respectively. The seedling height increment and the total biomass of LYG decreased the most by 95.42% and 50.71%, respectively. With the increase of stress concentration, the change trends of net photosynthetic rate, stomatal conductance, transpiration rate and chlorophyll content of four Pyrus betulaefolia Bunge families were the same and they all decreased, among which there indexes of YC decreased the least and those of LYG decreased the most. The membrane permeability of four families increased gradually and it decreased significantly at the level of 0.6%, among which the increases of LYG and QD14 were larger, reaching 231.11% and 238.97% respectively but the increase of YC and QD20 was smaller, reaching 67.77% and 98.42% respectively. The changes of intercellular carbon dioxide concentration of leaves of four families were different with the increase of salt treatment level. LYG and QD20 showed a trend from a decline to a rise and YC presented the downward trend all the time, while QD14 was opposite to YC. Through the factor analysis and membership function analysis, the salt tolerance of the four families was in order of Yancheng > Qingdao14 > Qingdao20 > Lianyungang.【Conclusion】There were significant differences in salt tolerance among four half-sib families of Pyrus betulaefolia. The comprehensive salt tolerance of Yancheng family was the strongest followed by Qingdao 14 and Qingdao 20 and the Lianyungang family was the weakest.

Key words: Pyrus betulaefolia Bunge, half-sib families, salt tolerance, subordination function method

CLC Number:

MIAO Lifei, YU Xiaojing, ZHANG Qiuyue, FENG Chaonian. Salt tolerance of four half-sib families of Pyrus betulaefolia Bunge from coastal areas[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(5): 157-166.

Figures/Tables 11

Table 1

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地区 area	家系 family	经度(E)/ (°) longitude	纬度(N)/ (°) latitude	海拔/m elevation	土壤电导率/ (μS·cm^-1) soil electric conductivity
青岛	QD14	120.004 906	35.769 905	47	90.33
青岛	QD20	120.004 952	35.769 994	50	99.00
连云港	LYG	118.955 925	35.011 831	151	50.00
盐城	YC	120.102 586	33.754 961	2	533.33

家系 family	盐处理/% treatment	地上干质量/g dry weight of shoot	根系干质量/g dry weight of root	总干质量/g total dry weight	根冠比/% radio of root to shoot
LYG	CK	7.01±0.63 a	4.29±0.59 a	11.3±0.23 a	0.62±0.14 b
	0.2	2.51±0.60 b	3.06±0.78 b	5.57±1.20 b	1.25±0.31 a
	0.4	2.16±0.19 bc	2.62±0.20 b	4.78±0.17 b	1.22±0.19 a
	0.6	1.57±0.24 c	1.64±0.16 c	3.22±0.20 c	1.06±1.06 b
	0.8	1.68±0.34 bc	0.95±0.12 c	2.63±0.46 c	0.57±0.05 c
家系 family	盐处理/% treatment	地上干质量/g dry weight of shoot	根系干质量/g dry weight of root	总干质量/g total dry weight	根冠比/% radio of root to shoot
YC	CK	6.68±0.03 a	3.93±0.18 a	10.61±0.21 a	0.59±0.03 b
	0.2	4.18±0.06 b	3.83±0.96 a	7.96±0.97 b	0.93±0.23 a
	0.4	4.29±0.60 b	3.3±0.15 ab	7.59±0.75 b	0.78±0.07 ab
	0.6	2.50±0.34 c	2.49±0.52 b	4.99±0.82 c	0.99±0.11 a
	0.8	2.39±0.39 c	1.32±0.39 c	3.71±0.77 c	0.54±0.09 b
QD14	CK	4.73±0.64 a	2.38±0.38 a	7.11±1.01 a	0.50±0.02 a
	0.2	2.97±0.35 b	1.70±0.30 ab	4.67±0.48 b	0.58±0.12 a
	0.4	2.04±0.51 c	1.43±0.51 b	3.47±0.89 bc	0.71±0.24 a
	0.6	1.36±0.20 c	0.99±0.41 bc	2.35±0.44 c	0.74±0.32 a
	0.8	1.46±0.10 c	0.63±0.24 c	2.09±0.22 c	0.43±0.18 a
QD20	CK	5.95±0.74 a	3.56±0.75 a	9.50±1.49 a	0.59±0.05 b
	0.2	3.94±0.24 b	3.13±0.60 a	7.07±0.43 b	0.80±0.20 b
	0.4	2.58±0.33 c	2.85±0.56 a	5.44±0.86 b	1.10±0.13 a
	0.6	1.82±0.26 cd	1.55±0.43 b	3.37±0.66 c	0.85±0.17 ab
	0.8	1.35±0.54 d	0.90±0.21 b	2.25±0.75 c	0.70±0.13 b

家系 family	R₁	R₂	R₃	R₄	R₅	R₆	R₇	R₈	R₉	R₁₀	R₁₁
LYG	0.040	0.194	0.283	0.482	0.525	0.701	3.187	0.172	0.161	1.008	0.220
YC	0.279	0.439	0.498	0.696	0.900	0.913	1.474	0.547	0.424	0.870	0.501
QD14	0.185	0.395	0.414	0.499	0.600	0.736	1.857	0.501	0.385	0.940	0.572
QD20	0.166	0.009	0.408	0.593	0.700	0.669	3.690	0.220	0.344	1.219	0.401

指标 index	R₁	R₂	R₃	R₄	R₅	R₆	R₇	R₈	R₉	R₁₀	R₁₁
R₁	1
R₂	0.533	1
R₃	0.998^**	0.478	1
R₄	0.825	0.222	0.833	1
R₅	0.894	0.350	0.897	0.988^*	1
R₆	0.756	0.786	0.720	0.745	0.808	1
R₇	-0.691	-0.980^*	-0.643	-0.372	-0.500	-0.838	1
R₈	0.842	0.880	0.810	0.475	0.603	0.784	-0.954^*	1
R₉	0.961^*	0.461	0.966^*	0.672	0.754	0.571	-0.627	0.826	1
R₁₀	-0.380	-0.970^*	-0.319	-0.173	-0.282	-0.784	0.913	-0.750	-0.261	1
R₁₁	0.826	0.584	0.820	0.368	0.487	0.448	-0.706	0.877	0.929	-0.370	1

因子 factor	R₁	R₂	R₃	R₄	R₅	R₆	R₇	R₈	R₉	R₁₀	R₁₁	贡献率P contribution rate
1	0.282	0.950	0.220	0.073	0.181	0.719	-0.874	0.691	0.170	-0.994	0.306	0.358
2	0.675	0.091	0.686	0.974	0.932	0.682	-0.221	0.290	0.488	-0.091	0.147	0.329
3	0.681	0.298	0.694	0.216	0.314	0.136	-0.432	0.662	0.856	-0.056	0.941	0.313

Salt tolerance of four half-sib families of Pyrus betulaefolia Bunge from coastal areas

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家系 family	X₁	X₂	X₃	U(1)	U(2)	U(3)	D
LYG	0.140	-0.598	-1.369	0.703	0.170	0.000	0.307
YC	0.809	1.260	0.080	1.000	1.000	0.605	0.876
QD14	0.494	-0.977	1.025	0.860	0.000	1.000	0.621
QD20	-1.443	0.315	0.263	0.000	0.577	0.682	0.403
权重 index weight				0.358	0.329	0.313

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[2]	JIA Qingbin, LIU Geng, ZHAO Jiali, LI Kuiyou, SUN Wensheng. Variation analyses of growth traits in half-sib families of Korean pine and superior families selection [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(4): 109-116.
[3]	CHEN Jia, GOU Jingyi, ZHAO Qi, HAN Qingqing, LI Huiping, YAO Dan, ZHANG Jinlin. Induced growth and salt tolerance of alfalfa by rhizobium strains from the rhizosphere of Haloxylon ammodendron [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(6): 99-110.
[4]	ZHANG Qun, JI Xiaoyu, HE Zihang, WANG Zhibo, TIAN Zengzhi, WANG Chao. Cloning and salt tolerance analysis of BpGRAS1 gene in Betula platyphylla [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 38-46.
[5]	JIANG Lei, LI Huanyong, ZHANG Qin, ZHANG Huilong, QIAO Yanhui, ZHANG Huaxin, YANG Xiuyan. Effects of arbuscular mycorrhiza fungi on the growth and physiological metabolism of Pyrus betulaefolia Bunge seedlings under saline-alkaline stress [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(6): 152-160.
[6]	PENG Yang, LU Yuetang, ZHAO Yang, XIAO Feng. A half-sib progeny test and juvenile selection of Trachycarpus fortunei [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(5): 78-84.
[7]	ZHOU Jie, HUANG Jing,XING Wei, WANG Baosong, HE Xudong, JIAO Zhongyi. Cloning and functional characterization of SlSIP from shrub Salix [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(01): 39-44.
[8]	CHEN Ying, LUO Yongya, QIU Nafei, WANG Ruiqi, SHENG Lili, CAO Fuliang. Effects of NaCl treatment on the growth, salt tolerance and flavonoids accumulation in Ginkgo biloba suspension cells [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(06): 45-50.
[9]	ZHANG Zihan, WANG Jiayuan, YU Fangyuan. Effects of NaCl stress on germination characteristics of Melia azedarach L. seeds from two provenances [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 38(05): 107-112.
[10]	LI Yuanyuan，YANG Guang，WEI Rui，SUN Yanshuang，GUO Yuhua, ZHANG Ruiping，LIU Guifeng*. TabZIP transferred Betula platyphylla generation and salt tolerance analysis [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2013, 37(05): 6-12.
[11]	WANG Chen,，YU Mukui*，WANG Zongxing ，ZHANG Cui，WU Tonggui，CHENG Xiangrong， TAO Jing. Difference of response to salt stress of nine clones of Catalpa bungei [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2011, 35(02): 20-24.
[12]	JIANG Zeping, XIAO Xiaojun,, HE Kaiyue*. Effects of NaCl stress on physiological characteristic and cell ultrastructure in leaves of two Broussonetia papyrifera varieties [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2010, 34(03): 77-82.