Effects of NaN3 on Sapium sebiferum seed germination and seedling growth

doi:10.3969/j.issn.1000-2006.202002044

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (4): 47-54.doi: 10.3969/j.issn.1000-2006.202002044

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Effects of NaN₃ on Sapium sebiferum seed germination and seedling growth

CHEN Li¹(), ZHU Chao¹, ZHU Qingxiang¹, WANG Cuiming¹, BAO Jiashu¹, MO Chen¹, SHI Tingting², WAN Zhibing¹()

^1.College of Life and Environment Sciences，Huangshan University，Huangshan 245041，China
^2.College of Landscape Architecture, Nanjing Forestry University，Nanjing 210037，China

Received:2020-02-20 Revised:2020-04-02 Online:2020-07-22 Published:2020-08-13
Contact: WAN Zhibing E-mail:Hsxycl@hsu.edu.cn;wanzb626@hsu.edu.cn

Abstract

Abstract: Objective

We investigated the effects of NaN₃ on Sapium sebiferum seed germination and seedling growth, so as to provide a theoretical basis for establishing a technical system for screening mutants of early quality of Sapium sebiferum.

Method

Eight contents of NaN₃ (0, 5, 10, 15, 20, 25, 30, 35 mmol/L) were tested to study the effects of NaN₃ solution concentration on the seed germination percentage and seedling growth. We treated the seeds of Sapium sebiferum by mutagenesis, measured the seedling rate, seedling growth index, biomass, basic photosynthetic parameters of leaves, SPAD value and ACI value of leaves, and compared the changes of their growth and photosynthetic capacity.

Result

The seed germination, seedling growth and the effects of photosynthetic capacity of Sapium sebiferum under different concentrations of NaN₃have significant differences. NaN₃ treatment had an inhibiting effect on the germination rate of Sapium sebiferum, with the increase of concentrations, the inhibitory effect was enhanced. The germination rate was the lowest at 35 mmol/L, and the inhibitory index reached 74.31%. Seedling height, biomass, total root length, specific surface area and root tip number of seedlings treated with 20 mmol/L were significantly higher than those treated with other concentrations, and the response trend to NaN₃ concentration was basically the same, showing a phenomenon of “low promoting and high inhibiting”. The net photosynthetic rate (P_n), stomatal conductance (G_s)， transpiration rate (T_r) and water utilization rate (E_WUE) of seedling leaves under NaN₃20 mmol/L treatment were the highest, significantly higher than the other treatments, and the intercellular carbon dioxide concentration (C_i) was the lowest. The effect of NaN₃ on the SPAD value and ACI value of the leaves increased first and then decreased with the increase of the concentration, and the effect on the SPAD value of the leaves was significantly greater than that of ACI value, but the 20 mmol/L treatment was the best and significantly greater than the other treatments.

Conclusion

With the increase of concentration of NaN₃, Sapium sebiferum seed germination rate is lower and lower, closing to the half lethal concentration which is 20—30 mmol/L,seedling growth and P_n, G_s, T_r, E_WUE, SPAD and ACI values show a trend of increasing initially and decreasing later, both C_i and root-cap ratio decrease initially and increase later, and have different responses to NaN₃. Through the comprehensive analysis, the suitable condition for NaN₃ mutation treatment of Sapium sebiferum seeds is 20 mmol/L for 6 h. These results provideu technical support for the research on NaN₃ mutagenesis breeding of Sapium sebiferum, and can also be used as a theoretical reference for the breeding of new varieties of Sapium sebiferum, the promotion of superior varieties and the production practice.

Key words: Sapium sebiferum, NaN₃ mutagenesis, seeding growth, photosynthetic characteristics, mutation breeding

CLC Number:

S722.1

CHEN Li, ZHU Chao, ZHU Qingxiang, WANG Cuiming, BAO Jiashu, MO Chen, SHI Tingting, WAN Zhibing. Effects of NaN₃ on Sapium sebiferum seed germination and seedling growth[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(4): 47-54.

Figures/Tables 5

Table 1

Fig. 1

Fig. 2

Table 2

Fig. 3

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处理 treatment	萌发率/% germination rate	苗高/cm seedling height	根生物量/g root biomass	茎叶生物量/g stem and leaf biomass	根冠比 root?shoot ratio
CK	61.00±11.37 a	107.37±2.69 b	5.47±0.41 b	14.74±1.75 de	0.38±0.05 ab
C1	54.00± 7.02 b	105.60± 7.15 b	5.74±0.25 b	20.18±0.22 c	0.28±0.01 bc
C2	44.67± 5.04 b	107.73± 3.90 b	5.50±0.60 b	18.08± 1.89 cd	0.32±0.06 abc
C3	37.00± 6.25 b	108.73± 1.01 b	7.77±0.74 a	29.25±1.31 b	0.27±0.04 bc
C4	33.67± 5.78 bc	124.63± 4.26 a	8.50±0.49 a	36.10±2.13 a	0.24±0.02 c
C5	34.00± 5.20 bc	123.00± 5.90 a	8.00±0.79 a	34.01±1.23 a	0.23±0.03 c
C6	31.00 ±1.53 b	98.90 ±2.29 bc	3.67±0.35 c	10.58±0.64 e	0.34±0.03 abc
C7	15.67± 1.20 c	88.63± 2.14 c	3.22±0.24 c	7.76± 0.98 e	0.43±0.07 a

处理 treatment	SPAD			ACI
	均值	抑制指数/%	总值	均值	抑制指数/%	总值
	mean value	inhibitory index	gross value	mean value	inhibitory index	gross value
CK	31.31±1.69 a	—	139.31±4.85	5.97±0.46 ab	—	23.86± 0.48
C1	32.47±1.43 b	-3.70	147.55±5.89	6.25±0.33 bc	-4.69	25.01± 0.28
C2	35.42±1.34 c	-13.13	154.64±3.80	6.28±0.34 c	-5.19	25.13± 0.10
C3	40.08±2.14 e	-28.01	161.97±1.29	6.60±0.30 d	-10.55	26.39±0.25
C4	43.21±2.81 f	-38.01	170.17±2.04	7.28±0.32 e	-21.94	29.11±0.27
C5	39.01±1.59 e	-24.59	164.02±1.71	6.59±0.21 d	-10.39	26.36±0.02
C6	36.78±1.35 d	-17.47	161.40±4.32	6.26±0.31 c	-4.86	25.04±0.30
C7	33.10±1.70 c	-5.72	146.08±4.39	5.85±0.15 a	2.01	23.39±0.12

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Effects of NaN₃ on Sapium sebiferum seed germination and seedling growth

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