Effects of bio-organic and microbial fertilizers on growth and soil nutrients of Vaccinium spp. seedlings

doi:10.3969/j.issn.1000-2006.201907049

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (6): 63-70.doi: 10.3969/j.issn.1000-2006.201907049

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Effects of bio-organic and microbial fertilizers on growth and soil nutrients of Vaccinium spp. seedlings

WANG Aibin¹(), SONG Huifang¹, ZHANG Liuyang², ZHANG Ming¹, YANG Shiwen¹, ZHANG Lingyun¹^,^*()

1. State Key Laboratory of Forest Cultivation and Protection of Ministry of Education, Beijing Forestry University, Beijing 100083, China
2. Hubei Provincial Forestry Investigation and Planning Institute, Wuhan 430079, China

Received:2019-07-31 Revised:2019-11-05 Online:2020-11-30 Published:2020-12-07
Contact: ZHANG Lingyun E-mail:996716070@qq.com;lyzhang@bjfu.edu.cn

Abstract

Abstract:

【Objective】This study aimed to investigate the effects of different bio-organic and microbial fertilizers on growth and soil nutrients of blueberry seedlings and screen out a suitable bio-organic fertilizer or microbial fertilizer for the growth and development of blueberry to provide a scientific basis for fertilizers and soil improvement in blueberry culture. 【Method】Considering two-year-old seedlings of blueberry variety ‘Brigita’ as test materials, we compared the effects of ferment residue (T1), EM compost (T2), Trichoderma spp. preparation (T3), Bacillus subtilis preparation (T4), and Avermectin organic fertilizer (T5) on the growth and different soil nutrients of blueberry from June to August during their growth period; there was no fertilization in the control treatment (CK). 【Result】The length and diameter growth of blueberry basal branches, dry weight of 100 leaves, leaf areas, number of basal branches, and crown width were improved remarkably under all the fertilization treatments. The content of N, P, K, soluble sugar, soluble protein, phenols, and chlorophyll in leaves also increased, barring the number of basal branches of T5 and soluble sugar content of T2 and T4 in leaves. The total N, total P, ammonium nitrogen, available K, and pH under the fertilizer treatment were lower than those under CK in the soil. However, the total K, available P, organic matters, and electrical conductivity (EC) values were higher than those under CK. The effects of Trichoderma preparation (T3) on length of blueberry basal branches, dry weight of 100 leaves, leaf areas, number of blueberry basal branches, crown width, leaf N content, leaf P content, soluble sugar content, phenol content, chlorophyll content of leaves, available K, organic matter in soil, and EC and pH of soil were the most evident. 【Conclusion】Altogether, the order of effects of different bio-organic and microbial fertilizers on the growth and soil nutrients of blueberry are as follows: T3 > T2 > T1 > T4 > T5> CK. These results also indicate that Trichoderma preparation can be used as an ideal fertilization to promote the growth of blueberry.

Key words: Vaccinium spp., bio-organic fertilizer, microbial fertilizer, principal component analysis(PCA), soil physicochemical properties

CLC Number:

S714

WANG Aibin, SONG Huifang, ZHANG Liuyang, ZHANG Ming, YANG Shiwen, ZHANG Lingyun. Effects of bio-organic and microbial fertilizers on growth and soil nutrients of Vaccinium spp. seedlings[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(6): 63-70.

Figures/Tables 7

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References 28

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种类 type	肥料名称 fertilizername	有益活性菌含量/CFU content of beneficial active bacteria	质量分数/% content							含水率/% moisture content
种类 type	肥料名称 fertilizername	有益活性菌含量/CFU content of beneficial active bacteria	有机质 organic matter	N		P		K		含水率/% moisture content
生物肥 bio-organic fertilizer	酵素菌肥(T1) ferment fertilizer	≥0.5	26		3.5		2.8		2.4	18
	EM菌堆肥(T2) EM bacteria compost	≥200.0	28		3.4		2.3		2.1	24
	阿维菌素有机肥(T5) Avermectin bio-organic fertilizer	≥0.2	45		≥5.0		≥5.0		≥5.0	—
菌肥 microbial fertilizer	木霉菌制剂(T3) Trichoderma spp.	5×10⁹	—		—		—		—	—
菌肥 microbial fertilizer	枯草芽孢杆菌制剂(T4) Bacillus subtilis	100.0	—		—		—		—	—

处理 treatment	养分含量 nutrient content								pH	土壤电导率/ (mS·cm^-1) EC
处理 treatment	全氮/ (g·kg^-1) TN	全磷/ (g·kg^-1) TP	全钾/ (g·kg^-1) TK	铵态氮/ (mg·kg^-1) ammonium-N	硝态氮/ (mg·kg^-1) nitrate-N	有效磷/ (mg·kg^-1) available-P	速效钾/ (mg·kg^-1) available-K	有机质/ (g·kg^-1) organic matter	pH	土壤电导率/ (mS·cm^-1) EC
CK	3.73±0.32 a	19.21±1.01 a	5.38±0.41 c	12.57±1.02 a	151.15±7.32 a	276.71±10.33 b	369.87±9.36 a	123.47±4.42 d	6.54±0.52 a	82.23±5.62 c
T1	2.95±0.27 b	5.43±0.41 d	5.83±0.40 b	9.67±0.85 b	135.72±6.23 c	334.16±12.99 a	334.31±16.33 bc	150.88±6.39 c	5.82±0.51 c	116.97±7.68 b
T2	2.82±0.12 b	4.59±0.33 d	5.41±0.48 c	8.29±0.74 c	134.04±6.22 c	317.67±10.36 a	320.36±14.42 c	169.33±5.55 b	5.75±0.44 c	120.47±4.36 b
T3	2.58±0.20 b	9.11±0.86 b	5.94±0.60 a	8.04±0.53 c	124.36±5.89 c	335.57±11.33 a	307.56±14.96 d	192.79±6.87 a	5.66±0.31 c	150.72±3.87 a
T4	2.64±0.13 b	8.80±0.62 c	5.56±0.33 bc	8.23±0.66 c	141.50±7.66 b	316.40±10.36 a	334.40±19.36 bc	132.00±6.56 d	6.03±0.72 b	109.18±6.88 b
T5	3.40±0.25 a	10.24±0.92 b	5.38±0.51 c	9.02±0.87 b	149.75±6.98 a	320.65±13.69 a	349.82±10.52 b	124.38±5.99 d	6.13±0.49 b	94.33±8.97 c

处理 treatment	基生枝条数 number of basal branches	冠幅/cm crown width	百叶干质量/g dry weight of 100 leaves	叶面积/ cm² leaf area
CK	3.00±0.63 c	7.46±1.59 c	1.05±0.03 b	8.10±0.54 c
T1	5.83±0.60 b	11.04±0.50 bc	1.59±0.24 b	23.69±0.10 b
T2	7.17±0.70 a	11.62±0.27 b	1.63±0.21 b	35.33±1.95 ab
T3	6.83±1.22 a	19.22±1.36 a	2.64±0.38 a	50.15±6.59 a
T4	4.17±1.11 bc	8.21±1.16 bc	1.41±0.14 b	39.12±4.20 ab
T5	1.83±0.31 c	9.51±1.61 bc	1.35±0.41 b	35.47±10.00 ab

处理 treatment	第1主成分(F1) primary principal component		第2主成分(F2) secondary principal component		综合(F) composite
处理 treatment	得分 score	排名 rank	得分 score	排名 rank	得分 score	排名 rank
CK	-1.676	6	0.989	2	-0.867	6
T1	-0.004	3	-0.206	3	-0.065	3
T2	0.575	1	-0.815	5	0.155	2
T3	-1.202	5	1.484	1	1.383	1
T4	0.014	2	-0.901	6	-0.263	4
T5	-0.251	4	-0.552	4	-0.342	5

Effects of bio-organic and microbial fertilizers on growth and soil nutrients of Vaccinium spp. seedlings

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