Effects of salicylic acid on germination of Cyclobalanopsis glauca seeds under NaHCO3 stress in Karst area of northwest Guangxi

doi:10.12302/j.issn.1000-2006.202008030

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (4): 114-122.doi: 10.12302/j.issn.1000-2006.202008030

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Effects of salicylic acid on germination of Cyclobalanopsis glauca seeds under NaHCO₃ stress in Karst area of northwest Guangxi

DENG Ping¹^,²(), ZHAO Ying¹, WANG Xia¹, CHEN Qiuyou¹, WU Min¹^,^*()

1. Yulin Normal University, Key Laboratory Breeding Base for the Protection and Utilization of Rare Economic Species in Southeastern Guangxi, Yulin 537000, China
2. College of Forestry, Northwest A & F University, Yangling 712100, China

Received:2020-08-19 Accepted:2021-01-10 Online:2021-07-30 Published:2021-07-30
Contact: WU Min E-mail:yier253160@126.com;wumin63843@126.com

Abstract

Abstract:

【Objective】This study aimed to alleviate NaHCO₃ stress in Cyclobalanopsis glauca by investigating the effects of exogenous application of salicylic acid (SA) on the seed germination and physiological characteristics, under bicarbonate stress. 【Method】Changes in germination rates and indexes, vigor indexes, salt tolerance indexes, relative salt damage rates, malondialdehyde (MDA) content, antioxidant superoxide dismutase (SOD), peroxidase (POD) and cata-lase (CAT) activities as well as osmotic adjustment substances agents (proline, soluble sugar and soluble protein) content were assessed in C. glauca seeds under NaHCO₃ (0, 24, 48, 72, 96, 120 and 144 mmol/L) stress and exogenous application of salicylic acid (SA: 0 and 0.50 mmol/L) treatment. 【Result】①Germination parameters and salt tole-rance were lower, and salt damage rates were significantly higher under NaHCO₃ stress, without SA, in C. glauca seeds compared to those of the control. However, seeds had higher germination parameters, a higher salt tolerance index, and lower relative salt damage rates under NaHCO₃ stress with SA compared to those without SA. The seed germination parameters and a salt tolerance index were always higher under 0-72 mmol/L NaHCO₃ stress with SA treatment compared with the control group. The germination parameters and a salt tolerance index were maximal, and the relative salt damage rate was minimal under 72 mmol/L NaHCO₃ stress with 0.50 mmol/L of SA treatment. ②The POD and CAT activities of seeds without SA initially increased, then decreased with increasing NaHCO₃ concentrations, and SOD activity was significantly lower than that of the control group, whereas the MDA content rapidly increased. Antioxidant enzyme activities and MDA contents were significantly higher and lower, respectively, under NaHCO₃ stress with, than without SA. The activities of SOD and POD were maximal in 72 mmol/L of NaHCO₃ with 0.50 mmol/L of SA. ③The contents of osmotic adjustment agents were high under NaHCO₃ stress without SA, and increased under NaHCO₃ stress with SA compared with the control. The contents of osmotic adjustment agents were maximal under 72 mmol/L of NaHCO₃ with 0.50 mmol/L of SA.【Conclusion】 The effects of 72 mmol/L NaHCO₃ stress on C. glauca seeds sowed and bred in the Karst areas were alleviated after immersion in 0.50 mmol/L exogenous SA.

Key words: Cyclobalanopsis glauca, seed germination, salicylic acid, NaHCO₃, Karst area

CLC Number:

Q945.79
S718

DENG Ping, ZHAO Ying, WANG Xia, CHEN Qiuyou, WU Min. Effects of salicylic acid on germination of Cyclobalanopsis glauca seeds under NaHCO₃ stress in Karst area of northwest Guangxi[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(4): 114-122.

Figures/Tables 6

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Table 1

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NaHCO₃ 浓度/ (mmol·L^-1) NaHCO₃ concentration	耐盐指数 salt tolerance index		相对盐害率 relative salt damage rate
NaHCO₃ 浓度/ (mmol·L^-1) NaHCO₃ concentration	0 mmol/L SA	0.50 mmol/L SA	0 mmol/L SA	0.50 mmol/L SA
0	100± 0.00 a	113.22± 7.25 b^*	0 g	-8.97± 0.97 f^**
24	40.92± 5.25 d	111.83± 13.54 b^**	17.24± 1.36 f	3.44± 0.53 d^**
48	60.93± 3.98 c	116.74± 9.85 b^**	20.67± 2.81 e	0.69± 0.10 e^**
72	79.55± 4.25 b	129.34± 10.39 a^**	24.13± 3.45 d	-10.35± 1.89 g^**
96	52.26± 5.48 c	91.90± 7.55 c^**	27.58± 4.01 c	17.24± 1.63 c^**
120	39.45± 2.89 d	75.94± 14.20 e^**	31.03± 2.78 b	24.13± 1.89 b^*
144	29.02± 5.12 e	63.87± 8.65 d^**	37.93± 1.89 a	34.48± 3.33 a

Effects of salicylic acid on germination of Cyclobalanopsis glauca seeds under NaHCO₃ stress in Karst area of northwest Guangxi

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