Study on ploidy and genome sizes of Osmanthus fragrans and its related species

doi:10.12302/j.issn.1000-2006.202104027

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (5): 47-52.doi: 10.12302/j.issn.1000-2006.202104027

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Study on ploidy and genome sizes of Osmanthus fragrans and its related species

DUAN Yifan¹^,²(), LI Lan¹, YANG Xinxin¹, WANG Xianrong¹^,², ZHANG Min¹^,², ZHANG Cheng¹, CHAI Zihan¹

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China
2. Cangnan Forestry Bureau of Zhejiang Province, Cangnan 325800,China

Received:2021-04-18 Accepted:2021-06-10 Online:2021-09-30 Published:2021-09-30

Abstract

Abstract:

【Objective】 This study aimed to provide new cytological evidence for the classification of Osmanthus fragrans and its related species (O. fortunei, O. heterophyllus and O. cooperi).【Method】 In this study, we used representative samples of Oryza sativa ‘Nipponbare’ to estimate the ploidy and genome sizes of 14 O. fragrans cultivars and three related species by using flow cytometry.【Result】 Average values of the O. fragrans Asiaticus Group, Luteus Group, Albus Group and Aurantiacus Group are 777.24, 760.85, 739.06 and 717.29 Mb, respectively. The largest genome size of the cultivar O. fragrans ‘Siji’ was 903.14 Mb. Genome sizes of O. cooperi and O. heterophyllus are 817.19 and 769.50 Mb, respectively; O. fortunei has the smallest genome size (729.11 Mb).【Conclusion】 Fourteen O. fragrans cultivars and three related species were diploid, but their genome sizes differed. The genome size of O. cooperi was close to that of O. fragrans ‘Xionghuang’, O. fragrans ‘Jinqiu’ and O. fragrans ‘Yuntian Cai.’ These results indicated a close relationship between O. cooperi and O. fragrans.

Key words: Osmanthus fragrans, close relationship, flow cytometry, ploidy identification, genome size

CLC Number:

S685
S718

DUAN Yifan, LI Lan, YANG Xinxin, WANG Xianrong, ZHANG Min, ZHANG Cheng, CHAI Zihan. Study on ploidy and genome sizes of Osmanthus fragrans and its related species[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 47-52.

Figures/Tables 4

Table 1

Fig.1

Fig.2

Table 2

References 30

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材料 materials	采集地 location	凭证标本编号 voucher specimen
‘四季’O. fragrans ‘Siji’	南京林业大学校内	SJG-01
‘小叶佛顶珠’O. fragrans ‘Xiaoye Fodingzhu’	常州市芳芝林现代农业产业园区	SJG-02
‘天香台阁’O. fragrans ‘Tianxiang Taige’	常州市芳芝林现代农业产业园区	SJG-03
‘雄黄’O. fragrans ‘Xionghuang’	南京林业大学校内	DG-01
‘蒲城丹’O. fragrans ‘Pucheng Dan’	常州市芳芝林现代农业产业园区	DG-02
‘齿丹’O. fragrans ‘Chi Dan’	常州市芳芝林现代农业产业园区	DG-03
‘金球’O. fragrans ‘Jinqiu’	南京林业大学校内	JG-01
‘波叶金’O. fragrans ‘Boye Jin’	南京林业大学校内	JG-02
‘速生金’O. fragrans ‘Susheng Jin’	常州市芳芝林现代农业产业园区	JG-03
‘早黄’O. fragrans ‘Zao Huang’	常州市芳芝林现代农业产业园区	YG-01
‘早银’O. fragrans ‘Zao Yin’	南京林业大学校内	YG-02
‘云田彩’O. fragrans ‘Yuntian Cai’	常州市芳芝林现代农业产业园区	CYG-01
‘虔南桂妃’O. fragrans ‘Qiannan Guifei’	常州市芳芝林现代农业产业园区	CYG-02
‘银碧双辉’O. fragrans ‘Yinbi Shuanghui’	常州市芳芝林现代农业产业园区	CYG-03
齿叶木犀O. × fortunei	常州市芳芝林现代农业产业园区	SPE-01
柊树O. heterophyllus	常州市芳芝林现代农业产业园区	SPE-02
华东木犀O. cooperi	常州市芳芝林现代农业产业园区	SPE-03

样品 samples	样品峰值(B) peak value of samples	水稻峰值(A) peak value of Oryza sativa	样品峰值/ 水稻峰值 ratio of B to A	样品估计基因组大小/Mb genome size	C值/pg C-value
‘四季’O. fragrans ‘Siji’	17 010	7 907	2.151	903.14 ± 3.87 m	0.92
‘小叶佛顶珠’O. fragrans ‘Xiaoye Fodingzhu’	25 136	15 097	1.665	697.55 ±6.11 c	0.71
‘天香台阁’O. fragrans ‘Tianxiang Taige’	19 882	11 438	1.738	731.04 ± 4.60 d	0.74
‘雄黄’O. fragrans ‘Xionghuang’	22 749	11 931	1.907	801.15 ± 2.12 i	0.82
‘蒲城丹’O. fragrans ‘Pucheng Dan’	16 607	10 586	1.569	657.97 ± 2.09 a	0.67
‘齿丹’O. fragrans ‘Chi Dan’	16 708	10 169	1.643	692.74 ± 2.53 bc	0.71
‘金球’O. fragrans ‘Jinqiu’	20 232	10 496	1.928	808.57 ± 4.45 j	0.83
‘波叶金’O. fragrans ‘Boye Jin’	22 276	11 891	1.873	787.10 ± 2.16 h	0.80
‘速生金’O. fragrans ‘Susheng Jin’	16 641	10 180	1.635	686.87 ± 1.10 b	0.70
‘早黄’O. fragrans ‘Zao Huang’	15 815	9 503	1.664	697.86 ± 2.92 c	0.71
‘早银’O. fragrans ‘Zao Yin’	19 820	10 655	1.860	780.25 ± 1.50 g	0.80
‘云田彩’O. fragrans ‘Yuntian Cai’	21 674	10 943	1.981	832.79 ± 2.26 l	0.85
‘虔南桂妃’O. fragrans ‘Qiannan Guifei’	18 655	10 247	1.821	765.31 ± 1.79 f	0.78
‘银碧双辉’O. fragrans ‘Yinbi Shuanghui’	16 061	9 032	1.778	747.19 ±1.30 e	0.76
齿叶木犀O. × fortunei	16 377	9 435	1.736	729.11 ± 2.73 d	0.74
柊树O. heterophyllus	20 118	10 977	1.832	769.50 ±3.12 f	0.79
华东木犀O. cooperi	19 058	9 788	1.947	817.19 ± 1.96 k	0.84

Study on ploidy and genome sizes of Osmanthus fragrans and its related species

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