Cloning and expression characteristics of ClWRKY44 gene from Cunninghamia lanceolata

doi:10.12302/j.issn.1000-2006.202011005

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (1): 203-209.doi: 10.12302/j.issn.1000-2006.202011005

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Cloning and expression characteristics of ClWRKY44 gene from Cunninghamia lanceolata

LIN Lili¹^,²^,³(), HU Anqi¹, CHEN Gang¹^,³, ZHANG Jiyue¹, CAO Guangqiu¹^,³, CAO Shijiang¹^,³^,^*()

1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. Huachuan Technology Company Limited, Fuzhou 350008, China
3. Chinese Fir Engineering Technology Research Center of the National Forestry and Grassland Administration, Fuzhou 350002, China

Received:2020-11-03 Accepted:2021-04-05 Online:2022-01-30 Published:2022-02-09
Contact: CAO Shijiang E-mail:1062676579@qq.com;csjiang1123@126.com

Abstract

Abstract:

【Objective】The aim of this study is to clone the Cunninghamia lanceolata ClWRKY44 gene and analyze its expression characteristics, so as to provide a theoretical basis for revealing the molecular mechanism of stress resistance physiology of Cunninghamia lanceolata. 【Method】 We cloned the Chinese fir ClWRKY44 gene by using RT-PCR technology, conducted bioinformatics analysis and subcellular localization study, and used real-time fluorescent quantitative PCR technology to analyze its expression. 【Result】The open reading frame (ORF)of ClWRKY44 gene is 1 776 bp, which encodes 591 amino acids and has a WRKY domain. Phylogenetic tree analysis showed that ClWRKY44 gene was closely related to Arabidopsis thaliana ATWRKY44. It was expressed in different organs (leaves, roots, stems) of Cunninghamia lanceolata. After 6 h of low temperature stress treatment, the relative expression of ClWRKY44 gene was the highest, about 79.8 times that of the control group. After 24 h of drought stress treatment, the relative expression of ClWRKY44 gene was the highest under high concentration drought stress treatment, about 46.6 times that of the control group. 【Conclusion】The protein sequence encoded by Chinese fir ClWRKY44 gene has high homology with Arabidopsis ATWRKY44. It can play a role in regulating plant stress response, and provide a reference for the adaptability of Chinese fir seedling growth and development.

Key words: Cunninghamia lanceolate, gene cloning, gene expression analysis, protein subcellular localization, stresses

CLC Number:

S722
Q751

LIN Lili, HU Anqi, CHEN Gang, ZHANG Jiyue, CAO Guangqiu, CAO Shijiang. Cloning and expression characteristics of ClWRKY44 gene from Cunninghamia lanceolata[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(1): 203-209.

Figures/Tables 9

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引物名称 primer number	引物序列 primer sequence (5'-3')
WRKY44Q-Fw	GGACAGAAAGTGGTGAAGGG
WRKY44Q-Rv	TAGTGGCGCATCATGGTTGT
Actin-Fw	CAGCAACTGGGATGATATGG
Actin-Rv	ATTTCGCTTTCAGCAGTGGT

Cloning and expression characteristics of ClWRKY44 gene from Cunninghamia lanceolata

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