Effects of different rootstocks on growth and photosynthetic characteristics of grafted seedlings of Catalpa bungei

doi:10.12302/j.issn.1000-2006.202201003

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (5): 97-106.doi: 10.12302/j.issn.1000-2006.202201003

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Effects of different rootstocks on growth and photosynthetic characteristics of grafted seedlings of Catalpa bungei

JIA Ruirui¹(), ZHU Yanyan¹, YANG Xiulian¹^,^*(), FU Yu¹, YUE Yuanzheng¹, WANG Lianggui¹^,²

1. Key Laboratory of Landscape Architecture of Jiangsu Province,College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037,China
2. Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China

Received:2022-01-05 Revised:2022-05-15 Online:2023-09-30 Published:2023-10-10

Abstract

Abstract:

【Objective】 This study mainly analyzed the differences in the growth indexes and photosynthetic characteristics of different Catalpa ovata and C. fargesii grafted seedlings in the first year, and studied the effect of different rootstocks on the growth of C. bungei grafted seedlings, to provide a basic reference for the preliminary screening of suitable rootstocks for C. bungei grafting and subsequent in-depth research.【Method】C. ovata and C. fargesii were used as rootstocks, and C. bungei ‘Nanlin 1’, C. bungei ‘Luoqiu’and C. bungei ‘Su 1’ were used as scions. There were six grafting combinations in total. The chlorophyll content, net photosynthetic rates and chlorophyll fluorescence parameters were measured at 128, 141, 155, 174 and 189 d after grafting. After 6 months, the survival rate of each grafting combination was counted. The height and diameter growth of scions in the current year and the ratio of upper to lower diameters of grafting interface after one year were measured.【Result】The survival rate of C. bungei ‘Nanlin 1’/C. fargesii was the highest among the six grafting combinations, reaching 76.91%. The survival rate of C. bungei ‘Su 1’/C. fargesii was the lowest, only 32.05%. The scion height and diameter growth of C. bungei ‘Nanlin 1’/ C. ovata were the largest, 165.51 and 2.89 cm, respectively. The scion height and diameter growth of C. bungei ‘Su 1’/C. fargesii were the smallest, 83.43 and 1.63 cm respectively. The ratio of upper to lower diameter of each grafting combination was less than 1, and there was no phenomenon of ‘big and small feet’ in the first year of grafting growth. However, the compatibility of C. bungei ‘Su 1’/C. fargesii was the worst. In the whole growth period, except for the grafting combination with C. bungei ‘Su 1’ as scion, the chlorophyll content, net photosynthetic rate (P_n), maximum photochemical efficiency of PS Ⅱ (F_v/F_m) and potential activity of PS Ⅱ (F_v/F_o) of the grafting combination with C. fargesii as rootstock were generally higher than those with C. ovata as rootstock. Different grafting combinations and growth periods had significant effects on P_n, the content of chlorophyll a, chlorophyll b and total chlorophyll the content of. 155 days after grafting, significant differences were among the combinations. P_n was significantly positively correlated with content of chlorophyll a, chlorophyll b and total chlorophyll (P < 0.05). C. bungei ‘Nanlin 1’ / C. fargesii and C. bungei ‘Luoqiu’ / C. fargesii had a high chlorophyll content, high energy conversion efficiency and strong photosynthetic ability. P_n was significantly positively correlated with F_m (maximum fluorescence) and F_v/F_o. P_n and F_v/F_o of C. bungei ‘Su 1’/C. fargesii and C. bungei ‘Su 1’/C. ovata combinations were lower and F_o (initial fluorescence) was higher, indicating that their ability to withstand strong light was weak. 【Conclusion】 To sum up, the preliminary judgment showed that when C. bungei ‘Nanlin 1’ and C. bungei ‘Luoqiu’ used C. fargesii as rootstock, the development potential was greater. While when C. bungei ‘Su 1’ used C. ovata as rootstock, the comprehensive performance was better. This study only analyzed the growth difference of each rootstock-scion combination in the first year of grafting. The growth condition in the later stage and whether there are ‘big and small feet’ need to be further explored.

Key words: Catalpa bungei, rootstock and scion combination, photosynthetic characteristics, chlorophyll fluorescence, growth state

CLC Number:

S688
S718

JIA Ruirui, ZHU Yanyan, YANG Xiulian, FU Yu, YUE Yuanzheng, WANG Lianggui. Effects of different rootstocks on growth and photosynthetic characteristics of grafted seedlings of Catalpa bungei[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(5): 97-106.

Figures/Tables 9

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References 39

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编号 No.	接穗 scion	砧木 rootstock	简称 abbreviation
1	‘南林1号’	梓树	NZ
2	‘洛楸’	梓树	LZ
3	‘苏楸1号’	梓树	SZ
4	‘南林1号’	滇楸	ND
5	‘洛楸’	滇楸	LD
6	‘苏楸1号’	滇楸	SD

嫁接组合 grafting combination	成活率/% survival rate	新梢高生长量/cm high scion growth	新梢粗生长量/cm scion diameter growth	嫁接口上下径粗比 ratio of scion diameter to stock diameter
NZ	59.08±1.46 c	165.51±4.85 a	2.89±0.92 a	0.933±0.124 a
ND	76.91±1.57 a	122.60±6.37 c	2.47±0.65 b	0.840±0.245 cd
LZ	72.35±2.25 b	159.06±11.05 a	2.34±1.09 b	0.885±0.071 bc
LD	73.22±3.76 b	139.25±14.32 b	2.27±1.69 b	0.891±0.065 b
SZ	60.95±2.33 c	107.51±12.25 d	1.94±1.30 c	0.822±0.029 d
SD	32.05±1.42 d	83.43±5.06 e	1.63±2.09 d	0.721±0.087 e

指标 index	嫁接组合 grafting combination		嫁接时间 grafting time		嫁接组合 × 嫁接时间 grafting combination× grafting time
指标 index	F	P	F	P	F	P
叶绿素a含量 Chl a content	92.231	<0.01	60.259	<0.01	7.265	<0.01
叶绿素b含量 Chl b content	83.321	<0.01	42.334	<0.01	5.126	<0.01
总叶绿素含量 Chl (a+b) content	90.587	<0.01	50.947	<0.01	3.888	<0.01
净光合速率 net photosynthetic rate	19.564	<0.01	204.873	<0.01	4.935	<0.01

嫁接时间/d grafting time	叶绿素a含量/(mg·g^-1) chlorophyll a content
嫁接时间/d grafting time	NZ	ND	LZ	LD	SZ	SD
128	1.826±0.065 Ab	1.875±0.059 Aa	1.825±0.025 Ab	1.807±0.032 Bb	1.654±0.067 Cc	1.624±0.041 BCc
141	1.853±0.042 Aa	1.876±0.041 Aa	1.695±0.033 Cb	1.859±0.030 Aa	1.709±0.046 Bb	1.657±0.042 Bc
155	1.862±0.053 Aa	1.852±0.037 Aa	1.751±0.041 Bc	1.851±0.031 Aa	1.786±0.024 Ab	1.736±0.036 Ac
174	1.805±0.014 Bb	1.844±0.036 ABa	1.689±0.032 Cc	1.791±0.036 Bb	1.714±0.025 Bc	1.592±0.040 Cd
189	1.788±0.025 Bb	1.835±0.029 Ba	1.586±0.025 Dd	1.642±0.040 Cc	1.382±0.034 Df	1.506±0.028 De

嫁接时间/d grafting time	叶绿素b含量/(mg·g^-1) chlorophyll b content
嫁接时间/d grafting time	NZ	ND	LZ	LD	SZ	SD
128	0.552±0.009 Bb	0.678±0.017 Ba	0.530±0.015 Ab	0.541±0.026 ABb	0.398±0.013 Bc	0.406±0.015 Bc
141	0.592±0.021 Bb	0.723±0.033 Aa	0.413±0.014 Bc	0.602±0.049 Ab	0.438±0.025 ABc	0.435±0.042 Ac
155	0.709±0.010 Aa	0.568±0.069 Cb	0.443±0.020 Bc	0.584±0.027 Ab	0.484±0.013 Ac	0.443±0.023 Ac
174	0.594±0.011 Ba	0.528±0.018 Cb	0.410±0.013 Bd	0.493±0.008 Bc	0.409±0.016 Bd	0.347±0.035 Ce
189	0.485±0.015 Cb	0.524±0.021 Ca	0.341±0.034 Cc	0.359±0.021 Cc	0.367±0.037 Cc	0.301±0.011 Cd

Effects of different rootstocks on growth and photosynthetic characteristics of grafted seedlings of Catalpa bungei

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嫁接时间/d grafting time	总叶绿素含量/(mg·g^-1) total chlorophyll content
嫁接时间/d grafting time	NZ	ND	LZ	LD	SZ	SD
128	2.395±0.093 Bb	2.553±0.086 Aa	2.355±0.056 Ab	2.348±0.049 ABb	2.051±0.050 Cc	2.030±0.112 Bc
141	2.445±0.086 ABb	2.598±0.085 Aa	2.109±0.098 Bc	2.461±0.092 Ab	2.147±0.109 Bc	2.092±0.082 Bc
155	2.571±0.060 Aa	2.419±0.056 Bb	2.196±0.047 Bd	2.436±0.059 Ab	2.269±0.039 Ac	2.180±0.085 Ad
174	2.425±0.055 Ba	2.372±0.100 Bab	2.101±0.096 Bc	2.285±0.079 Bb	2.123±0.096 BCc	1.939±0.071 Cd
189	2.273±0.064 Cb	2.359±0.052 Ba	1.926±0.044 Bd	2.006±0.105 Cc	1.749±0.054 Df	1.814±0.060 De

嫁接时间/d grafting time	净光合速率/(μmol·m^-2·s^-1) net photosynthetic rate
嫁接时间/d grafting time	NZ	ND	LZ	LD	SZ	SD
128	11.35±0.50 Bc	13.25±0.63 Ba	12.27±0.72 Bb	12.88±0.64 Cab	11.23±0.41 Cc	9.77±0.45 Bd
141	15.93±0.71 Aa	14.23±0.60 Bb	13.07±1.67 Bab	14.65±1.23 Bab	13.67±1.58 Bb	14.05±2.39 Aab
155	15.77±0.69 Acd	18.47±0.45 Aa	16.83±2.51 Abc	17.26±1.10 Aab	15.17±0.51 Ad	13.62±0.43 Ae
174	10.78±1.38 BCbc	11.40±1.10 Cb	10.76±1.02 Cbc	13.73±2.19 BCa	10.68±1.32 Cbc	9.48±0.80 Bc
189	9.83±0.49 Cab	10.45±0.85 Cab	9.32±0.33 Cbc	9.53±0.93 Dbc	8.88±0.73 Dc	6.77±0.55 Cd

指标index	Chla	Chlb	Chl(a+b)	F_o	F_m	F_v/F_m	F_v/F_o	P_n
Chla	1
Chlb	0.855^*	1
Chl(a+b)	0.966^*	0.959^*	1
F_o	-0.054	-0.148	-0.104	1
F_m	0.599^*	0.495^*	0.578^*	0.096	1
F_v/F_m	0.428^*	0.416^*	0.436^*	0.078	0.486^*	1
F_v/F_o	0.433^*	0.409^*	0.430^*	0.269	0.358	0.859^*	1
P_n	0.587^*	0.551^*	0.584^*	0.084	0.424^*	0.315	0.406^*	1

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