Screening and analysis of differential secondary metabolites in Castanea mollissima with different levels of resistance to Oligonychus ununguis

doi:10.12302/j.issn.1000-2006.202209053

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2024, Vol. 48 ›› Issue (2): 234-240.doi: 10.12302/j.issn.1000-2006.202209053

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Screening and analysis of differential secondary metabolites in Castanea mollissima with different levels of resistance to Oligonychus ununguis

ZHANG Xinfang(), WANG Guangpeng^*(), ZHANG Shuhang, LI Ying, GUO Yan

Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, China

Received:2022-09-25 Revised:2023-03-24 Online:2024-03-30 Published:2024-04-08

Abstract

Abstract:

【Objective】 The differential secondary metabolites in chestnut (Castanea mollissima) leaves with different mite resistance levels were screened to provide a reference for the analysis of the mite(Oligonychus ununguis) resistance mechanism and the breeding of highly mite-resistant chestnut varieties.【Method】 The chestnut varieties ‘Yanxing’ and ‘Likang’ were used to identify and determine the mite resistance levels to O. ununguis with the field investigation method. Metabolomics detection was performed using ultra-high-performance liquid chromatography and tandem mass spectrometry, and the differential metabolites were screened using a combination of fold change and the variable importance in projection value. The R software (ComplexHeatmap and MetaboAnalystR package) was used to draw the clustering heat map and orthogonal partial least squares-discriminant analysis score map. Identified metabolites were annotated using the Kyoto encyclopedia of genes and genomes (KEGG) database, and the annotation results were classified by pathway types.【Result】 The resistance levels of ‘Likang’ and ‘Yanxing’ to O. ununguis were high resistance and susceptible, respectively. A total of 704 secondary metabolites were detected, including 165 differential metabolites. The content of 73 metabolites in ‘Likang’ was significantly higher than in ‘Yanxing’, and the content of 92 metabolites was lower in ‘Likang’ than in ‘Yanxing’. The types of differential metabolites included 56 phenolic acids, 60 flavonoids, 19 lignins and coumarins, four tannins, five alkaloids, 16 terpenoids and five other types. Flavonoids and phenolic acids accounted for 36% and 34%, respectively. Metabolites present only in ‘Likang’ included caffeoylcholine-4-O-glucoside, 3-hydroxy-5-methylphenol-1-O-(6'-galloyl)glucoside, tricin-7-O-glucuronide, 6'-trans-cinnamoyl-8-epikingisidic acid, lariciresinol-4'-O-glucoside, albanol B and sanguiin H11. There were 15 metabolites present only in ‘Yanxing’, including 4-methyl-5-thiazoleethanol, 2,4-dihydroxybenzoic acid, isofraxidin, salicin and roxburic acid. Thirty-three differential metabolites in ‘Yanxing’ and ‘Likang’ were annotated to 12 metabolic pathways.【Conclusion】 The secondary metabolic profiles between the mite-susceptible variety ‘Yanxing’ and the highly resistant variety ‘Likang’ differed. Secondary metabolites that may be related to chestnut mite resistance were prunetin, epigallocatechin, caffeic acid, ferulic acid, several lignans and 4-methyl-5-thiazoleethanol. The differential secondary metabolites were mainly annotated and enriched in the flavonoid biosynthesis pathway and the flavone and flavonol biosynthesis pathway.

Key words: Castanea mollissima, Oligonychus ununguis, mite resistance, secondary metabolites

CLC Number:

S718
S763.3

ZHANG Xinfang, WANG Guangpeng, ZHANG Shuhang, LI Ying, GUO Yan. Screening and analysis of differential secondary metabolites in Castanea mollissima with different levels of resistance to Oligonychus ununguis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 234-240.

Figures/Tables 5

Table 1

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References 25

[1]	温素卿. 燕山地区板栗针叶小爪螨的重发原因分析及综合防治[J]. 河北果树, 2021(2):50-52.
	WEN S Q. Cause analysis and comprehensive control of the recurrence of Tetranychus viennensis in chestnut in Yanshan area[J]. Hebei Fruits, 2021(2):50-52.DOI: 10.19440/j.cnki.1006-9402.2021.02.030.
[2]	张文辉, 刘光杰. 植物抗虫性次生物质的研究概况[J]. 植物学通报, 2003, 20(5):522-530.
	ZHANG W H, LIU G J. A review on plant secondary substances in plant resistance to insect pests[J]. Chin Bull Bot, 2003, 20(5):522-530.
[3]	NICHOLSON J K, LINDON J C. Metabonomics[J]. Nature, 2008, 455(7216):1054-1056.DOI: 10.1038/4551054a.
[4]	袁平丽, 何楠, 赵胜杰, 等. 籽瓜、黏籽和普通西瓜的果实代谢组比较[J]. 中国农业科学, 2021, 54(19):4179-4195.
	YUAN P L, HE N, ZHAO S J, et al. Metabolomics comparative study on fruits of edible seed watermelon,egusi and common watermelon[J]. Sci Agric Sin, 2021, 54(19):4179-4195.DOI: 10.3864/j.issn.0578-1752.2021.19.013.
[5]	CHEN W, GONG L, GUO Z L, et al. A novel integrated method for large-scale detection,identification,and quantification of widely targeted metabolites:application in the study of rice metabolomics[J]. Mol Plant, 2013, 6(6):1769-1780.DOI: 10.1093/mp/sst080.
[6]	TRIPODI P, CARDI T, BIANCHI G, et al. Genetic and environmental factors underlying variation in yield performance and bioactive compound content of hot pepper varieties (Capsicum annuum) cultivated in two contrasting Italian locations[J]. Eur Food Res Technol, 2018, 244(9):1555-1567.DOI: 10.1007/s00217-018-3069-5.
[7]	王景顺, 吴秋芳, 路志芳. 植物次生代谢物与林木抗虫性研究进展[J]. 江苏农业科学, 2015, 43(8):4-7.
	WANG J S, WU Q F, LU Z F. Research progress on plant secondary metabolites and insect resistance of forest trees[J]. J Jiangsu Agric Sci, 2015, 43(8):4-7.DOI: 10.15889/j.issn.1002-1302.2015.08.002.
[8]	轩静渊, 王辅. 植物抗虫性概论[M]. 成都: 四川科学技术出版社,1991.
	XUAN J Y, WANG F. Introduction to plant insect resis-tance[M]. Chengdu: Sichuan Scientific & Technical Publishers,1991.
[9]	李菁, 骆有庆, 石娟, 等. 利用植物源引诱剂监测与控制舞毒蛾[J]. 北京林业大学学报, 2011, 33(4):85-90.
	LI J, LUO Y Q, SHI J, et al. Use of phyto-attractant in monitoring and controlling gypsy moth[J]. J Beijing For Univ, 2011, 33(4):85-90.DOI: 10.13332/j.1000-1522.2011.04.010.
[10]	左彤彤, 迟德富, 王牧原, 等. 不同品系杨树酚酸类物质对青杨脊虎天牛的驱避作用[J]. 植物保护学报, 2008, 35(2):160-164.
	ZUO T T, CHI D F, WANG M Y, et al. Repellent effects of phenolic acids in different strain poplars to Xylotrechus rusticus[J]. J Plant Prot, 2008, 35(2):160-164.DOI: 10.3321/j.issn:0577-7518.2008.02.013.
[11]	杨振德, 赵博光. 苦豆子生物碱对柳蓝叶甲产卵行为的影响[J]. 林业科学, 2013, 49(1):152-160.
	YANG Z D, ZHAO B G. Effects of alkaloids from Sophora alopecuroides on oviposition behavior of Plagiodera versicolora[J]. Sci Silvae Sin, 2013, 49(1):152-160.DOI: 10.11707/j.1001-7488.20130122.
[12]	栾风福, 刘玉刚, 鲁刚, 等. 板栗新品种丽抗的选育[J]. 中国果树, 2003(4):1-2.
	LUAN F F, LIU Y G, LU G, et al. Breeding of a new chestnut variety ‘Likang’[J]. China Fruits, 2003(4):1-2.DOI: 10.16626/j.cnki.issn1000-8047.2003.04.001.
[13]	张继亮, 马玉敏, 孙海伟, 等. 栗红蜘蛛对板栗不同品种的危害调查[J]. 落叶果树, 2004, 36(5):50-51.
	HANG J L, MA Y M, SUN H W, et al. Investigation on damage of chestnut red mite to different chestnut varieties[J]. Deciduous Fruits, 2004, 36(5):50-51.DOI: 10.13855/j.cnki.lygs.2004.05.027.
[14]	雷恒久, 苏淑钗, 张海成. 板栗对板栗红蜘蛛和桃蛀螟的抗性机制初探[J]. 河北林果研究, 2009, 24(1):73-76.
	LEI H J, SU S C, ZHANG H C. A preliminary study on the resistance mechanism of chestnut to Oligonychus ununguis(Jacobi) and Dichocrocis punctiferalis Guenee[J]. Hebei J For Orchard Res, 2009, 24(1):73-76.DOI: 10.3969/j.issn.1007-4961.2009.01.020.
[15]	刘庆忠, 孙山, 张力思, 等. 板栗种质资源描述规范和数据标准[M]. 北京: 中国农业出版社, 2006:67-68.
	LIU Q Z, SUN S, ZHANG L S, et al. Descriptors and data standard for chestnut (Castanea mollissima Blume)[M]. Beijing: China Agriculture Press, 2006:67-68.
[16]	焦蕊, 许长新, 于丽辰, 等. 针叶小爪螨的研究进展[J]. 河北农业科学, 2013, 17(1):61-64.
	JIAO R, XU C X, YU L C, et al. Research progress of Oligonychus ununguis Jacobi[J]. J Hebei Agric Sci, 2013, 17(1):61-64.DOI: 10.16318/j.cnki.hbnykx.2013.01.008.
[17]	ZHANG P P, DU H Y, WANG J, et al. Multiplex CRISPR/Cas9-mediated metabolic engineering increases soyabean isoflavone content and resistance to soya bean mosaic virus[J]. Plant Biotechnol J, 2020, 18(6):1384-1395.DOI: 10.1111/pbi.13302.
[18]	MURATA K, KITANO T, YOSHIMOTO R, et al. Natural variation in the expression and catalytic activity of a naringenin 7-O-methy-ltransferase influences antifungal defenses in diverse rice cultivars[J]. Plant J, 2020, 101(5):1103-1117.DOI: 10.1111/tpj.14577.
[19]	SEYBOLD H, DEMETROWITSCH T J, HASSANI M A, et al. A fungal pathogen induces systemic susceptibility and systemic shifts in wheat metabolome and microbiome composition[J]. Nat Commun, 2020, 11(1):1910.DOI: 10.1038/s41467-020-15633-x.
[20]	DAI Z Y, TAN J, ZHOU C, et al. The OsmiR396-OsGRF8-OsF3H-flavonoid pathway mediates resistance to the brown planthopper in rice (Oryza sativa)[J]. Plant Biotechnol J, 2019, 17(8):1657-1669.DOI: 10.1111/pbi.13091.
[21]	RANJAN A, WESTRICK N M, JAIN S, et al. Resistance against Sclerotinia sclerotiorum in soybean involves a reprogramming of the phenylpropanoid pathway and up-regulation of antifungal activity targeting ergosterol biosynthesis[J]. Plant Biotechnol J, 2019, 17(8):1567-1581.DOI: 10.1111/pbi.13082.
[22]	李树安. 肉香型香料4-甲基-5-噻唑乙醇的合成[J]. 精细化工, 2005, 22(7):521-523.
	LI S A. Synthesis of meat flavor perfume 4-methyl-5-thiazoleethanol[J]. Fine Chem, 2005, 22(7):521-523.DOI: 10.3321/j.issn:1003-5214.2005.07.011.
[23]	刘玲玲, 武彦文, 袁亚荣, 等. 鸡味脂肪香精的制备、香气成分分析和抗氧化性研究[J]. 食品与发酵工业, 2010, 36(10):107-111.
	LIU L L, WU Y W, YUAN Y R, et al. The preparation,aromatic components analysis and antioxidation activity of chicken fat flavor[J]. Food Ferment Ind, 2010, 36(10):107-111.DOI: 10.13995/j.cnki.11-1802/ts.2010.10.025.
[24]	FANG C Y, FERNIE A R, LUO J. Exploring the diversity of plant metabolism[J]. Trends Plant Sci, 2019, 24(1):83-98.DOI: 10.1016/j.tplants.2018.09.006.
[25]	WANG S C, ALSEEKH S, FERNIE A R, et al. The structure and function of major plant metabolite modifications[J]. Mol Plant, 2019, 12(7):899-919.DOI: 10.1016/j.molp.2019.06.001.

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等级level	螨害情况mite condition
0	叶片不失绿或几乎不失绿leaf blade is almost green
1	叶片失绿面积占叶片总面积的25%以下leaf chlorosis area accounts for less than 25% of total leaf area
2	叶片失绿面积占叶片总面积的26%~50% leaf chlorosis area accounts for 26%-50% of total leaf area
3	叶片失绿面积占叶片总面积的51%~75% leaf chlorosis area accounts for 51%-75% of total leaf area
4	叶片失绿面积占叶片总面积的76%~100% leaf chlorosis area accounts for 76%-100% of total leaf area

Screening and analysis of differential secondary metabolites in Castanea mollissima with different levels of resistance to Oligonychus ununguis

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