Effects of AMF on photosynthetic characteristics of Phoebe zhennan under salt stress

doi:10.12302/j.issn.1000-2006.202005007

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (1): 101-106.doi: 10.12302/j.issn.1000-2006.202005007

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Effects of AMF on photosynthetic characteristics of Phoebe zhennan under salt stress

CUI Lingjun(), LIU Yuxia, LIN Jian, SHI Kaiming^*()

Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei Minzu University, Enshi 445000, China

Received:2020-05-06 Accepted:2020-11-09 Online:2021-01-30 Published:2021-02-01
Contact: SHI Kaiming E-mail:lingjuncui@163.com;skm331@163.com

Abstract

Abstract:

【Objective】 The effects of arbuscular mycorrhizal fungi (AMF) and salt stress on the photosynthetic pigment content, gas exchange parameters and chlorophyll fluorescence parameters of Phoebe zhennan were studied to provide a reference for afforestation using P. zhennan in saline-alkali land. 【Method】 The contents of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids (Car) were determined by spectrophotometry, and the gas exchange parameters and chlorophyll fluorescence parameters were determined using a Li-6400 portable photosynthesis instrument. 【Result】Under the different concentrations of salt stress, the contents of Chl a, Chl b and Car in the leaves of P. zhennan were significantly different between the AMF and non-AMF treatments, and the AMF significantly increased the chlorophyll content. The net photosynthetic rate (P_n), stomatal conductance (G_s), intercellular CO₂ concentration (C_i) and transpiration rate (T_r) in the leaves of P. zhennan with the AMF treatment were significantly higher than those of the non-AMF treatment. The maximal photochemical efficiency of photosystem Ⅱ (F_v/F_m), PSⅡ quantum yield (Φ_PSⅡ), and photochemical quenching coefficient (qP) of the AMF treatment leaves under salt stress did not change significantly, whereas those in the non-AMF treatment leaves decreased significantly. The non-photochemical quenching coefficient (NPQ) of non-AMF treatment leaves increased significantly at 300 mmol/L salt concentration, while the AMF treatment leaves were not significantly changed. The two-way analysis of variance showed that the AMF had significant effects on the contents of Chl a, Chl b, Car and Φ_PSⅡ. 【Conclusion】The AMF improves the utilization of light energy by P. zhennan by increasing the photosynthetic pigment content, gas exchange parameters, and chlorophyll fluorescence parameters, thereby reducing salt damage to P. zhennan. This shows that the AMF can enhance the salt tolerance of P. zhennan and promote its growth in saline-alkaline soils.

Key words: Phoebe zhennan, arbuscular mycorrhizal fungi (AMF), salt stress, photosynthetic characteristics

CLC Number:

S718

CUI Lingjun, LIU Yuxia, LIN Jian, SHI Kaiming. Effects of AMF on photosynthetic characteristics of Phoebe zhennan under salt stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(1): 101-106.

Figures/Tables 4

Table 1

Table 2

Fig.1

Fig.2

References 27

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NaCl浓度/ (mmol·L^-1) NaCl concentration	叶绿素a含量/(mg·g^-1) Chl a content		叶绿素b含量/(mg·g^-1) Chl b content		叶绿素a/b Chl a/Chl b		类胡萝卜素含量/(mg·g^-1) Car content
NaCl浓度/ (mmol·L^-1) NaCl concentration	+AMF	-AMF	+AMF	-AMF	+AMF	-AMF	+AMF	-AMF
0	2.894±0.265 a	2.534±0.264 a	1.187±0.245 a	1.082±0.213 a	2.438±0.248 a	2.342±0.185 a	1.668±0.126 a	1.517±0.157 a
100	2.945±0.358 b	2.456±0.382 b	1.194±0.211 b	1.014±0.128 b	2.422±0.339 b	2.384±0.206 b	1.704±0.178 b	1.463±0.186 b
200	2.687±0.412 b	2.262±0.164 bc	1.175±0.143 a	0.919±0.126 c	2.287±0.247 c	2.461±0.182 a	1.571±0.181 b	1.422±0.177 b
300	2.445±0.218 b	1.883±0.177 c	0.981±0.127 b	0.873±0.112 c	2.492±0.324 a	2.157±0.231 c	1.314±0.176 c	1.264±0.163 c

处理 treatment	叶绿素a Chl a	叶绿素b Chl b	叶绿素 a/b Chl a/ Chl b	类胡萝卜素 Car	P_n	G_s	C_i	T_r	F_v/F_m	Φ_PSⅡ	光化学淬灭系数 qP	非光化学淬灭系数 NPQ
AMF	**	*	*	*	NS	NS	NS	NS	NS	*	NS	NS
NaCl	NS	NS	*	NS	**	*	*	**	NS	**	*	NS
AMF×NaCl	NS	NS	NS	*	*	**	*	*	*	NS	NS	NS

Effects of AMF on photosynthetic characteristics of Phoebe zhennan under salt stress

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