Cryopreservation of embryogenic callus of Pinus elliottii × Pinus caribaea using control freezing

doi:10.3969/j.issn.1000-2006.2013.06.001

Cryopreservation of embryogenic callus of Pinus elliottii × Pinus caribaea using control freezing

LIU Weidong, CHEN Jinhui, ZHOU Yanwei, ZHAO Yaqi, SHI Jisen

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2013, Vol. 37 ›› Issue (06) : 1-5.

PDF(815674 KB)

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2013, Vol. 37 ›› Issue (06) : 1-5. DOI: 10.3969/j.issn.1000-2006.2013.06.001

Cryopreservation of embryogenic callus of Pinus elliottii × Pinus caribaea using control freezing

LIU Weidong, CHEN Jinhui, ZHOU Yanwei, ZHAO Yaqi, SHI Jisen^*

Author information +

History +

Abstract

Cryopreservation of callus of Pinus elliottii × Pinus caribaea were conducted in this research. The results showed that the best process of cryopreservation for the embryogenic callus of P. elliottii × P. caribaea was as follows: the callus were sub-cultured for 9-12 days in callus proliferation medium firstly and were pre-cultured in 0.5 mol/L sorbol for 4 days, and then pretreated 20 minutes in buffer of 0.6 mol/L sorbol + 10% DMSO, cooled to -40 ℃ at rate of -1 ℃/min, maintained at -40 ℃ for 10 min, preserved in liquid nitrogen after cooled to -90 ℃ at rate of -5 ℃/min. The resuscitate callus were kept in 37 ℃ water bath, clean three times in 1 mol/L sorbol liquid medium, passage in solid medium support by filter paper. New embryogenic callus will be produced within one month.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LIU Weidong, CHEN Jinhui, ZHOU Yanwei, ZHAO Yaqi, SHI Jisen. Cryopreservation of embryogenic callus of Pinus elliottii × Pinus caribaea using control freezing[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(06): 1-5 https://doi.org/10.3969/j.issn.1000-2006.2013.06.001

References

[1] 陈代喜,莫钊志,李贵玉,等.杂交松育苗对比试验效果分析[J].广西林业科学,2002,31(2):76-77. Chen D X, Mo Z Z, Li G Y, et al. The controlled experiment on Pinus elliottii × Pinus oaribaea seedling-raising[J]. Guangxi Forestry Science,2002,31(2):76-77.
[2] 庞正轰,陈代喜,莫钊志,等.澳大利亚杂交松产脂量及松脂成分研究[J].林业科技开发,2009,23(6):23-26. Pang Z H, Chen D X, Mo Z Z, et al. Study on the output of resin and its chemical composition from Australia pine hybrid [J]. China Forestry Science and Technology, 2009, 23(6): 23-26.
[3] 凌绍明.澳大利亚杂交松在广西引种试验研究[J].广西林业科学,2007,36(4):192-195. Ling S M. A study on the introduction of Australia hybrid pine in Guangxi[J]. Guangxi Forestry Science,2007, 36(4): 192-195.
[4] 方利娟.杂交松组织培养技术研究[D].广西: 广西大学,2006. Fang L J. Study on technology about the tissue culture of Pinus elliottii×P.caribaea[D]. Guangxi:Guangxi University, 2006.
[5] 施翔.杂交松组织培养研究[D].南京: 南京林业大学,2005. Shi X. Study on tissue culture of Pinus elliottii×P.caribaea[D]. Nanjing: Nanjing Forestry University, 2005.
[6] 项东云,王以红,吴幼媚, 等.杂交松组培技术研究初探[J].广西林业科学,2009, 38(3):134-136. Xiang D Y, Wang Y H, Wu Y M, et al. Preliminary study on tissue culture of Pinus elliottii×P.caribaea[J].Guangxi Forestry Science,2009, 38(3):134-136.
[7] 施季森,于敏,边黎明,等.湿地松和加勒比松杂种体细胞胚胎发生和植株再生方法. CN200710132707.8[P]. 北京:知识产权出版社,2008. Shi J S, Yu M, Bian L M, et al. Method for somatic embryogenesis and plant regeneration of hybrid between Pinus elliotti and Pinus caribaea. CN200710132707.8[P].Beijing: Intellectual Property Publishing House,2008.
[8] 李峰卿.湿加杂种松体细胞胚胎发生同步化控制和植株再生[D].南京: 南京林业大学, 2009. Li F Q. Synchronization controlling system for the embryogenesis proliferationand plantlets regeneration of the new hybrid between Pinus elliottii and Pinus caribaea[D]. Nanjing: Nanjing Forestry University, 2009.
[9] 叶芳,夏胜,梅兴国.红豆杉愈伤组织超低温保存有关因素的研究[J].武汉植物学研究,2001,19(4):327-331. Ye F, Xia S, Mei X G. Studies on factors in cryopreservation of Taxus callus [J]. Journal of Wuhan Botanical Research,2001,19(4):327-331.
[10] Nieves V, Conchi S, Lorena J, et al. Cryopreservation of chestnut by vitrification of in vitro-grown shoot tips[J]. In Vitro Cellular & Developmental Biology-Plant,2005,41(1):63-68.
[11] 史峰厚, 喻方圆, 沈永宝, 等. 超低温贮存对油松种子的影响[J].南京林业大学学报:自然科学版, 2005, 29(6): 119-122. Shi F H, Yu F Y, Shen Y B, et al. Cryopreservation of chinesepine seeds[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2005, 29(6): 119-122.
[12] 任淑娟, 王航, 刘浩, 等. 七叶树种子离体胚的超低温冷冻保存[J].南京林业大学学报:自然科学版, 2010, 34(2): 19-23. Ren S J, Wang H, Liu H, et al. Cryopreservation of excised embryos of Aesculus chinensis seeds [J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2010, 34(2): 19-23.
[13] 施季森. 迎接21世纪现代林木生物技术育种的挑战[J].南京林业大学学报, 2000, 24(1): 1-6. Shi J S. The challenges to forestry biotechnology and tree breeding in the 21st century[J]. Journal of Nanjing Forestry University, 2000, 24(1): 1-6.
[14] 邵崇斌.概率论与数理统计[M].北京:中国林业出版社,2005.
[15] 徐刚标,易文,李美娥, 等.银杏愈伤组织超低温保存的研究[J].林业科学,2001, 37(3):30-34. Xu G B, Yi W, Li M E, et al. The studies of cryopreservation of callus for maidenhair tree(Gingko bilobal L.)[J]. Scientia Silvae Sinicae,2001, 37(3):30-34.
[16] 苗琦,谷运红,王卫东, 等.植物组织培养物的超低温保存[J].植物生理学通讯,2005,41(3): 350-354. Miao Q, Gu H Y, Wang W D, et al.The cryopreservation of plant tissue culture materials [J].Plant Physiology Journal, 2005, 41(3): 350-354.
[17] Karlsson J O, Toner M. Long-term storage of tissues by cryopreservation: critical issues [J]. Biomaterials, 1996, 17(3):243-256.
[18] 关静,龚承元,崔占峰.玻璃化冷冻保存细胞组织研究进展[J].国外医学:生物医学工程分册,2004, 27(4):252-256. Guan J, Gong C Y, Cui Z F.Recent advances in cryopreservation of cells and tissues by vitrification [J]. International Journal of Biomedical Engineering,2004, 27(4):252-256.
[19] 翟晓巧,程斐,朱延林.二乔刺槐愈伤组织超低温保存及适宜降温方法[J].林业科学,2009, 45(10):49-54. Zhao X Q, Cheng F, Zhu Y L. Methods of lowering temperature for cryopreservation of calli of Robinia bella-rosea[J]. Scientia Silvae Sinicae, 2009, 45(10):49-54.
[20] 刘剑锋,阎秀峰,程云清,等.高山红景天愈伤组织的超低温保存[J].林业科学,2007, 43(6):57-60. Liu J F, Yan X F, Cheng Y Q, et al. Cryopreservation of callus of Rhodiolasa chalinensis[J].Scientia Silvae Sinicae,2007, 43(6):57-60.
[21] 李广武,郑从义,唐兵.低温生物学[M].长沙:湖南科学技术出版社,1998.
[22] Barbara M Reed. Plant Cryopreservation: A Practical Guide[M]. New York:Springer, 2007.
[23] Malabadi R B, Nataraja K. Cryopreservation and plant regeneration via somatic embryogenesis using shoot apical domes of mature Pinus roxburghii sarg. trees[J]. In Vitro Cellular & Developmental Biology-Plant, 2006, 42(2):152-159.
[24] Mathur G, Alkuter V A, Nadgauda R S. Cryopreservation of embryogenic culture of Pinus roxburghii[J]. Biologia Plantarum, 2003, 46(2):205-210.
[25] Salaj T, Panis B, Swennen R, et al. Cryopreservation of embryogenic tissues of Pinus nigra Arn. by a slow freezing method[J]. CryoLetters, 2007, 28(2):69-76.
[26] Gale S, John A, Benson E E. Cryopreservation of Picea sitchensis(Sitka spruce)embryogenic suspensor masses[J]. CryoLetters, 2007,28(4):225-239.
[27] krlep K, Bergant M, De Winter G M. Cryopreservation of cell suspension cultures of Taxus media and Taxus floridana[J]. Biologia Plantarum, 2008, 52(2):329-333.
[28] 叶芳,夏胜,梅兴国.红豆杉愈伤组织超低温保存有关因素的研究[J].武汉植物学研究,2001,19(4):327-331. Ye F, Xia S, Mei X G. Studies on factors in cryopreservation of Taxus callus [J]. Journal of Wuhan Botanical Research,2001,19(4):327-331.
[29] 王高,范现丽,申晓辉,等.红松胚性愈伤组织的超低温保存技术[J].上海交通大学学报:农业科学版,2009,27(3):223-230. Wang G, Fan X L, Shen X H, et al. Cryopreservation of Korean pine somatic calli [J].Journal of Shanghai Jiaotong University:Agricultural Science,2009,27(3):223-230.

PDF(815674 KB)

Accesses

Citation

Detail

Sections

Recommended

The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

〈

〉

Please choose a citation manager

Content to export