Identification and analysis of latex rubber particle proteins related with tapping panel dryness in Hevea brasiliensis

doi:10.3969/j.issn.1000-2006.2014.01.007

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (01): 36-40.doi: 10.3969/j.issn.1000-2006.2014.01.007

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Identification and analysis of latex rubber particle proteins related with tapping panel dryness in Hevea brasiliensis

YUAN Kun¹, ZHOU Xuemei^1,2, WANG Zhenhui¹, YANG Lifu^1*

1.Rubber Research Institute, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Danzhou 571737, China;
2.College of Agriculture, Hainan University, Haikou 570100, China

Online:2014-01-15 Published:2014-01-15

Abstract

Abstract: The tapping panel dryness(TPD)in Hevea brasiliensis is one of the important factors influencing natural rubber production. In order to distinguish and identify the differentially expressed proteins of latex rubber particles in healthy and TPD trees, the two-dimensional electrophoresis(2-DE)was used. 35 differentially expressed protein spots were obtained by software analysis. Subjecting to MALDI-TOF MS following by searching for Uniprot rubber database, 13 protein spots were successfully identified, Of them, REF, FPS, GPX, GR, TCTP and HSP were mainly involed in the rubber biosynthesis, reactive oxygen metabolism and cell apoptosis processes. This study further showed that the pathways of the rubber biosynthesis, reactive oxygen metabolism and cell apoptosis were possibly the key regulated ones of TPD and which would provide a theoretical basis for elucidating the TPD mechanism in Hevea brasiliensis.

CLC Number:

S794.1

YUAN Kun, ZHOU Xuemei, WANG Zhenhui, YANG Lifu. Identification and analysis of latex rubber particle proteins related with tapping panel dryness in Hevea brasiliensis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 38(01): 36-40.

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Identification and analysis of latex rubber particle proteins related with tapping panel dryness in Hevea brasiliensis

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