Abstract: The contrast test on physiological index was conducted between the bamboos of Fargesia yunnanensis from wild environment and the same bamboos cultured in greenhouse for 6 years. The results showed that the chlorophyll a and chlorophyll b contents in young leaves, mature leaves and old leaves in high temperature were higher than bamboos in wild environment, but the chlorophyll a/b ratio was lower. The content of carotenoid in young leaves and mature leaves in greenhouse was higher than bamboos in wild environment except for the old leaves. The contents of soluble sugar in young and mature leaves in greenhouse were higher than bamboos in wild environment, but the SOD activity in mature and old leaves was lower than bamboos in wild environment, except for the young leaves. However, the CAT activity in mature leaves in greenhouse was lower. The MDA contents in both young and mature leaves were higher than bamboos in wild environments except for the old leaves. It could be concluded that F. yunnanesnis still couldn’t completely adapt to the environment of high temperature although it had been planted in greenhouse for long time.