Study on somatic embryogenesis regeneration system in Camellia oleifera

HU Yuling, YAO Xiaohua, REN Huadong, WANG Kailiang, LONG Wei

Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2014, Vol. 38 ›› Issue (06) : 160-164.

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Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2014, Vol. 38 ›› Issue (06) : 160-164. DOI: 10.3969/j.issn.1000-2006.2014.06.030

Study on somatic embryogenesis regeneration system in Camellia oleifera

  • HU Yuling, YAO Xiaohua*, REN Huadong, WANG Kailiang, LONG Wei
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Abstract

In order to establish genetic transformation receptor system for oil camellia plant via agrobacterium-mediated, ‘Changlin' 4,53 and 166 immature zygotic embryos were used as explants to carry out multi-level and multi-factor tissue culture experiments,the results showed that: the effect of culture medium pH on the callus induction rate was not significant, but varieties had a significant effect on callus induction rate. Somatic embryo regeneration was observed from ‘Changlin' 53. MS and 2,4-D and TDZ were suitable for the embryo regeneration, the somatic embryogenesis induction rate was 98%, using MS+2 mg/L 2,4-D+0.5 mg/L TDZ, the somatic embryo induction rate was 89.6% based on MS+1 mg/L 2,4-D+2 mg/L KT+500 mg/L CH, and in addition 2,4-D, KT, TDZ and additives experiments, the somatic embryogenesis induction rate of ‘Changlin' 53 was 100%. In hormone-free MS medium, the differentiation embryos could regenerate to plant.

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HU Yuling, YAO Xiaohua, REN Huadong, WANG Kailiang, LONG Wei. Study on somatic embryogenesis regeneration system in Camellia oleifera[J]. Journal of Nanjing Forestry University (Natural Sciences Edition). 2014, 38(06): 160-164 https://doi.org/10.3969/j.issn.1000-2006.2014.06.030

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