Study on regeneration system of Cryptomeria clones in vitro culture

ZHU Chenchen, XU Jin, OUYANG Lei, SHI Jisen

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2015, Vol. 39 ›› Issue (03) : 55-58.

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南京林业大学学报（自然科学版）
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Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2015, Vol. 39 ›› Issue (03) : 55-58. DOI: 10.3969/j.issn.1000-2006.2015.03.011

Study on regeneration system of Cryptomeria clones in vitro culture

  • ZHU Chenchen1,2, XU Jin1,2*, OUYANG Lei3, SHI Jisen1,2
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Abstract

The regeneration system in this paper was established by using soft stem of Cryptomeria as explants. Using orthogonal experimental design, among which six Cryptomeria clones were used for the bud proliferation and three clones were used for rooting induction. Main conclusions were shown as follows:① Adventitious bud could be induced by six different clones on DCR basic medium supplement with different combinations of 6-BA and NAA. But there are differences in bud proliferation ability in the different clones. The bud proliferation coefficient was up to 5.00(Jing 1#).② During the process of rooting culture of Cryptomeria clones, 1/2DCR medium was the optimum basic culture medium. And the rooting rate of three clones which had been experimented all reached the highest when added 0.10 mg/L IBA and 0.20 mg/L NAA. Among them, the rooting rate of 011# clone was up to 93.33%. The research will provide technical support for shortening the breeding cycle of the clones of Cryptomeria.

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ZHU Chenchen, XU Jin, OUYANG Lei, SHI Jisen. Study on regeneration system of Cryptomeria clones in vitro culture[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2015, 39(03): 55-58 https://doi.org/10.3969/j.issn.1000-2006.2015.03.011

References

[1] 陈正华.木本植物组织培养及其应用[M].北京:高等教育出版社,1986.
[2] 潘瑞炽.植物生理学[M].3版.北京:高等教育出版社,1995:48-52.
[3] 陈正华.木本植物组织培养及其应用[M].北京:高等教育出版社,1986:456-466.
[4] 中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,2004:293-295.
[5] 蔡燕康,陈观岩.柳杉扦插育苗试验[J].福建林业科技,2003,30(4):81-83.Cai Y K,Chen G Y. The experiment on cottage seedling raising of Cryptomeria citriodora[J]. Jour of Fujian Forestry Sci and Tech,2003,30(4):81-83.
[6] 张翠萍,方伟,桂仁意,等.柳杉试管快繁技术的研究[J].林业科技开发,2008,22(6):36-39.Zhang C P,Fang W,Gui R Y, et al. Studies on mass propagation in vitro of Cryptomeria fortune Hooibrenk ex Otto et Dietr by tissue culture[J]. China Forestry Science and Technology, 2008,22(6):36-39.
[7] 崔德才,徐培文.植物组织培养与工厂化育苗[M].北京:化学工业出版社,2003.
[8] 高小坤.杉木组培无根苗瓶外生根试验[J].福建林业科技,2006,33(4):163-165.Gao X K. Research on the ex-vitro rooting for tes-t tube rootless seedlings of Cunninghamia lanceolata[J]. Jour of Fujian Forestry Sci and Tech, 2006,33(4):163-165.
[9] 欧阳磊,郑仁华,翁玉榛,等.杉木优良无性系组培快繁技术体系的建立[J].南京林业大学学报:自然科学版,2007,31(3):47-51.Ouyang L, Zheng R H, Weng Y Z, et al. Establishment of technique system of tissue culture on Chinese fir superior clones[J].Journal of Nanjing Forestry University:Natural Sciences Edition, 2007,31(3):47-51.
[10] 张宇,卫志明,席梦利,等.马尾松高效再生体系的建立[J].分子细胞生物学报,2006,39(3):271-274.Zhang Y, Wei Z M, Xi M L, et al.Efficient plant regeneration in vitro in Pinus massoniana L.[J]. Journal of Molecular Cell Biolo,2006,39(3):271-274.
[11] Mathur G, Nadgauda R. In vitro plantlet regeneration from mature zygotic embryos of Pinus wallichiana A.B.Jacks[J]. Plant Cell Rep,1999,19:74-80.
[12] 罗向东,戴亮芳,罗建林,等,濒危黄杜鹃的离体快速繁殖体系研究[J].西北植物学报,2010,30(4):645-651.Luo X D,Dai L F,Luo J L,et al. In vitro micropropagation of the endangered Rhododendron molle G.Don[J]. Acta Botanica Boreali-Occidentalia Sinica, 2010,30(4):0645-0651.
[13] 于永明,王军辉,马建伟,等.楸树无性系离体培养特性差异研究[J]. 西北植物学报,2012,32(1):199-204Yu Y M, Wang J H, Ma J W, et al. Study on characteristic differences of Catalpa bungei clones in vitro culture[J]. Acta Botanica Boreali-Occidentalia Sinica, 2012,32(1):199-204.
[14] Lin X F, Zhang W B, Takechi K. Stable genetic transformation of Larix gmelinii L. by particle bombardment of zygotic embryos [J]. Plant Cell Rep, 2005,24(7):418-425.
[15] 郑成木,刘进平.热带亚热带植物微繁殖[M].长沙:湖南科技出版社,2001:12-28.
[16] 黄翠,景丹龙,王玉兵,等. 水杉愈伤组织诱导及植株再生[J].植物学报,2010,45(5):604-608.Huang C, Jing D L, Wang Y B, et al. Callus induction and plant regeneration of a living fossil, Metasequoia glyptostroboides[J]. Chinese Bulletin of Botany, 2010,45(5):604-608.
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