Influence of illumination intensity and provenance on triterpenoid accumulation in the leaves of Cyclocarya paliurus seedlings

LI Yan, ZHOU Xiaodong, FANG Shengzuo, DENG Bo, SHANG Xulan, YANG Wanxia

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (05) : 52-58.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (05) : 52-58. DOI: 10.3969/j.issn.1000-2006.2015.05.009

Influence of illumination intensity and provenance on triterpenoid accumulation in the leaves of Cyclocarya paliurus seedlings

  • LI Yan1,2, ZHOU Xiaodong1,3, FANG Shengzuo1*, DENG Bo1, SHANG Xulan1, YANG Wanxia1
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Abstract

Based on the seedlings of Cyclocarya paliurus(1-year stem with 2 year root system), split-split plot design with three C. paliurus provenances(Wufeng, Yuanling and Muchuan)and three light conditions(full sunlight, 50% full sunlight and 15% full sunlight)was adopted to compare the content variation of total triterpenoid, arjunolic acid and cyclocarioside I in the leaves of C. paliurus seedlings under different treatments. Meanwhile, the seasonal variation of triterpenoid contents in the leaves was also discussed. The results showed that there were significant differences in triterpenoid accumulation in leaves of C. paliurus seedlings among different treatments when October is defined as leaf harvest time. The highest content of total triterpenoids(42.03 mg/g)was obtained in the treatment of Yuanling provenance with full sunlight, while the greatest contents of arjunolic acid and cyclocarioside I were achieved under the treatments of Muchuan provenance with 15% of full sunlight and Muchuan provenance with full sunlight, reaching 4.42 mg/g and 2.52 mg/g, respectively. The greatest production of total triterpenoids and cyclocarioside I was obtained in the treatment of Muchuan provenance with full sunlight, reaching 2 907.2 and 264.4 mg/plant respectively. However, the highest production of arjunolic acid was observed in the treatments of Yuanling provenance with 50% of full sunlight, hitting to 208.6 mg/plant. The accumulation of triterpenoids in the leaves of Cyclocarya paliurus are closely related to provenance, illumination intensity and the harvest time of leaves. Results from this study would provide some important practical and theoretical bases for effectively managing the plantations of C. paliurus.

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LI Yan, ZHOU Xiaodong, FANG Shengzuo, DENG Bo, SHANG Xulan, YANG Wanxia. Influence of illumination intensity and provenance on triterpenoid accumulation in the leaves of Cyclocarya paliurus seedlings[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(05): 52-58 https://doi.org/10.3969/j.issn.1000-2006.2015.05.009

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