Cytological mechanism of unreduced 2n pollen formation in  Populus euphratica

doi:10.3969/j.issn.1000-2006.2015.06.008

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (06): 40-44.doi: 10.3969/j.issn.1000-2006.2015.06.008

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Cytological mechanism of unreduced 2n pollen formation in Populus euphratica

ZHAO Chenguang, LI Yanjie, ZHANG Pingdong^*, KANG Xiangyang

National Engineering Laboratory for Tree Breeding, Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants, Ministry of Education,Beijing Forestry University, Beijing 100083, China

Online:2015-11-30 Published:2015-11-30

Abstract

Abstract: In order to analyze abnormal behaviors of chromosomes during meiosis and abnormal organization of microtubule during meiosis and cytokinesis, and find a new method to illustrate unreduced 2n pollen formation, indirect immunofluorescence microscopy with DAPI staining was conducted to observe the microtubule cytoskeleton pattern and chromosomes behavior during meiosis and cytokinesis in Populus euphratica. The results indicated that: ① During the prophase stage of meiosis, the paired homologous chromosomes combined, loosely, with univalent at diakinesis. Parallel spindle, tripolar spindle and fused spindle could also been found, at metaphase Ⅱ Lagging chromosomes could be observed at anaphaseⅠand anaphaseⅡ. ② Positive correlations between frequency of 2n pollen and rate of tripolar spindle and fused spindle were found, which suggested that tripolar spindle and fused spindle could result in FDR-typed 2n pollen formation. ③ Prematurely cytokinesis was observed at prophaseⅡ, which resulted in SDR-typed 2n pollen formation in Populus euphratica. ④ Due to lack of spindle microtubule in one daughter cell at anaphaseⅡ, the chromatids do not migrated to the poles instead of retaining in the daughter cell and forming a SDR-typed 2n pollen.

CLC Number:

Q343
S792.11

ZHAO Chenguang, LI Yanjie, ZHANG Pingdong, KANG Xiangyang. Cytological mechanism of unreduced 2n pollen formation in Populus euphratica[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(06): 40-44.

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Cytological mechanism of unreduced 2n pollen formation in Populus euphratica

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